Restriction map for
pFN21AE1827
(Linear) (Six-base) MAPSORT of: KIEE1827.seq Check: 9088 from: 1 to: 1035
KIEE1827.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AccI GT'mk_AC
Cuts at: 0 157 1035
Size: 157 878
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 518 910 1035
Size: 503 15 392 125
Fragments arranged by size:
503 392 125 15
AgeI A'CCGG_T
Cuts at: 0 943 1035
Size: 943 92
AleI CACnn'nnGTG
Cuts at: 0 976 1035
Size: 976 59
AlwNI CAG_nnn'CTG
Cuts at: 0 508 1035
Size: 508 527
ApoI r'AATT_y
Cuts at: 0 85 1035
Size: 85 950
BbsI GAAGACnn'nnnn_
Cuts at: 0 296 596 1035
Size: 296 300 439
Fragments arranged by size:
439 300 296
BbvCI CC'TCA_GC
Cuts at: 0 131 1035
Size: 131 904
BciVI GTATCCnnnnn_n'
Cuts at: 0 248 1035
Size: 248 787
BglI GCCn_nnn'nGGC
Cuts at: 0 657 1035
Size: 657 378
BglII A'GATC_T
Cuts at: 0 639 1035
Size: 639 396
BmgBI CAC'GTC
Cuts at: 0 978 1035
Size: 978 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 335 1035
Size: 335 700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1035
Size: 1006 29
Bpu10I CC'TnA_GC
Cuts at: 0 131 1035
Size: 131 904
BsaI GGTCTCn'nnnn_
Cuts at: 0 927 1035
Size: 927 108
BsaBI GATnn'nnATC
Cuts at: 0 432 1035
Size: 432 603
BsaWI w'CCGG_w
Cuts at: 0 943 1035
Size: 943 92
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 742 1012 1035
Size: 742 270 23
Fragments arranged by size:
742 270 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 1035
Size: 732 303
BsiHKAI G_wGCw'C
Cuts at: 0 992 1027 1035
Size: 992 35 8
Fragments arranged by size:
992 35 8
Bsp1286I G_dGCh'C
Cuts at: 0 992 1027 1035
Size: 992 35 8
Fragments arranged by size:
992 35 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 560 1035
Size: 560 475
BsrDI GCAATG_nn'
Cuts at: 0 879 1035
Size: 879 156
BsrFI r'CCGG_y
Cuts at: 0 40 943 1035
Size: 40 903 92
Fragments arranged by size:
903 92 40
BssSI C'ACGA_G
Cuts at: 0 916 1035
Size: 916 119
BstXI CCAn_nnnn'nTGG
Cuts at: 0 620 705 1035
Size: 620 85 330
Fragments arranged by size:
620 330 85
BstYI r'GATC_y
Cuts at: 0 126 639 708 1035
Size: 126 513 69 327
Fragments arranged by size:
513 327 126 69
EaeI y'GGCC_r
Cuts at: 0 38 42 811 1035
Size: 38 4 769 224
Fragments arranged by size:
769 224 38 4
EarI CTCTTCn'nnn_
Cuts at: 0 13 1035
Size: 13 1022
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 79 1035
Size: 79 956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 518 910 1006 1035
Size: 503 15 392 96 29
Fragments arranged by size:
503 392 96 29 15
EcoO109I rG'GnC_Cy
Cuts at: 0 921 1035
Size: 921 114
EcoRV GAT'ATC
Cuts at: 0 604 1035
Size: 604 431
FseI GG_CCGG'CC
Cuts at: 0 44 1035
Size: 44 991
HaeII r_GCGC'y
Cuts at: 0 366 1035
Size: 366 669
MfeI C'AATT_G
Cuts at: 0 867 1035
Size: 867 168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 608 1035
Size: 95 513 427
Fragments arranged by size:
513 427 95
MscI TGG'CCA
Cuts at: 0 813 1035
Size: 813 222
MslI CAynn'nnrTG
Cuts at: 0 487 976 1035
Size: 487 489 59
Fragments arranged by size:
489 487 59
MspA1I CmG'CkG
Cuts at: 0 746 1035
Size: 746 289
NaeI GCC'GGC
Cuts at: 0 42 1035
Size: 42 993
NdeI CA'TA_TG
Cuts at: 0 307 1035
Size: 307 728
NgoMIV G'CCGG_C
Cuts at: 0 40 1035
Size: 40 995
PflMI CCAn_nnn'nTGG
Cuts at: 0 809 1035
Size: 809 226
PpuMI rG'GwC_Cy
Cuts at: 0 921 1035
Size: 921 114
PshAI GACnn'nnGTC
Cuts at: 0 946 1035
Size: 946 89
PvuII CAG'CTG
Cuts at: 0 746 1035
Size: 746 289
SspI AAT'ATT
Cuts at: 0 445 1035
Size: 445 590
StyI C'CwwG_G
Cuts at: 0 181 584 1035
Size: 181 403 451
Fragments arranged by size:
451 403 181
TatI w'GTAC_w
Cuts at: 0 120 1035
Size: 120 915
XmnI GAAnn'nnTTC
Cuts at: 0 446 1035
Size: 446 589
Enzymes that do cut:
AccI AcuI AgeI AleI AlwNI ApoI BbsI BbvCI
BciVI BglI BglII BmgBI BmrI BpmI Bpu10I BsaI
BsaBI BsaWI BseRI BsgI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BssSI BstXI BstYI EaeI EarI EciI Eco57MI
EcoO109I EcoRV FseI HaeII MfeI MmeI MscI MslI
MspA1I NaeI NdeI NgoMIV PflMI PpuMI PshAI PvuII
SspI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BcgI BclI
BfrBI BlpI Bme1580I BmtI BplI BpuEI BsaAI BsaHI
BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI FalI FspI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MluI NarI NcoI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI