Restriction map for
pFN21AE1821
(Linear) (Six-base) MAPSORT of: KIEE1821.seq Check: 4144 from: 1 to: 1005
KIEE1821.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 843 1005
Size: 843 162
AatII G_ACGT'C
Cuts at: 0 565 1005
Size: 565 440
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1005
Size: 673 332
AfeI AGC'GCT
Cuts at: 0 973 1005
Size: 973 32
AflII C'TTAA_G
Cuts at: 0 42 1005
Size: 42 963
AflIII A'CryG_T
Cuts at: 0 202 827 1005
Size: 202 625 178
Fragments arranged by size:
625 202 178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 785 1005
Size: 785 220
ApaLI G'TGCA_C
Cuts at: 0 856 1005
Size: 856 149
ApoI r'AATT_y
Cuts at: 0 322 1005
Size: 322 683
AvaI C'yCGr_G
Cuts at: 0 440 1005
Size: 440 565
BamHI G'GATC_C
Cuts at: 0 384 1005
Size: 384 621
BanII G_rGCy'C
Cuts at: 0 366 1005
Size: 366 639
BclI T'GATC_A
Cuts at: 0 327 1005
Size: 327 678
BlpI GC'TnA_GC
Cuts at: 0 758 1005
Size: 758 247
Bme1580I G_kGCm'C
Cuts at: 0 860 1005
Size: 860 145
BmrI ACTGGGnnnn_n'
Cuts at: 0 87 1005
Size: 87 918
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 680 1005
Size: 43 637 325
Fragments arranged by size:
637 325 43
BsaHI Gr'CG_yC
Cuts at: 0 562 1005
Size: 562 443
BsaWI w'CCGG_w
Cuts at: 0 790 819 1005
Size: 790 29 186
Fragments arranged by size:
790 186 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 520 1005
Size: 136 384 485
Fragments arranged by size:
485 384 136
BseYI C'CCAG_C
Cuts at: 0 365 1005
Size: 365 640
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1005
Size: 755 250
BsiHKAI G_wGCw'C
Cuts at: 0 860 1005
Size: 860 145
Bsp1286I G_dGCh'C
Cuts at: 0 366 860 1005
Size: 366 494 145
Fragments arranged by size:
494 366 145
BspEI T'CCGG_A
Cuts at: 0 790 1005
Size: 790 215
BspHI T'CATG_A
Cuts at: 0 936 1005
Size: 936 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 813 843 1005
Size: 813 30 162
Fragments arranged by size:
813 162 30
BstYI r'GATC_y
Cuts at: 0 241 384 1005
Size: 241 143 621
Fragments arranged by size:
621 241 143
DraI TTT'AAA
Cuts at: 0 612 1005
Size: 612 393
EaeI y'GGCC_r
Cuts at: 0 29 191 253 1005
Size: 29 162 62 752
Fragments arranged by size:
752 162 62 29
EarI CTCTTCn'nnn_
Cuts at: 0 935 1005
Size: 935 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 673 680 1005
Size: 43 630 7 325
Fragments arranged by size:
630 325 43 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 254 356 1005
Size: 254 102 649
Fragments arranged by size:
649 254 102
EcoO109I rG'GnC_Cy
Cuts at: 0 51 1005
Size: 51 954
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 57 602 1005
Size: 57 545 403
Fragments arranged by size:
545 403 57
HaeII r_GCGC'y
Cuts at: 0 975 1005
Size: 975 30
HindIII A'AGCT_T
Cuts at: 0 301 584 845 1005
Size: 301 283 261 160
Fragments arranged by size:
301 283 261 160
MluI A'CGCG_T
Cuts at: 0 202 1005
Size: 202 803
MscI TGG'CCA
Cuts at: 0 193 255 1005
Size: 193 62 750
Fragments arranged by size:
750 193 62
MslI CAynn'nnrTG
Cuts at: 0 427 1005
Size: 427 578
MspA1I CmG'CkG
Cuts at: 0 520 1005
Size: 520 485
NspI r_CATG'y
Cuts at: 0 788 831 1005
Size: 788 43 174
Fragments arranged by size:
788 174 43
PasI CC'CwG_GG
Cuts at: 0 54 1005
Size: 54 951
PciI A'CATG_T
Cuts at: 0 827 1005
Size: 827 178
PflMI CCAn_nnn'nTGG
Cuts at: 0 975 1005
Size: 975 30
PspXI vC'TCGA_Gb
Cuts at: 0 440 1005
Size: 440 565
PstI C_TGCA'G
Cuts at: 0 950 1005
Size: 950 55
PvuII CAG'CTG
Cuts at: 0 520 1005
Size: 520 485
SapI GCTCTTCn'nnn_
Cuts at: 0 935 1005
Size: 935 70
SfcI C'TryA_G
Cuts at: 0 946 1005
Size: 946 59
SmlI C'TyrA_G
Cuts at: 0 42 440 1005
Size: 42 398 565
Fragments arranged by size:
565 398 42
SphI G_CATG'C
Cuts at: 0 788 1005
Size: 788 217
StyI C'CwwG_G
Cuts at: 0 250 287 983 1005
Size: 250 37 696 22
Fragments arranged by size:
696 250 37 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 755 1005
Size: 755 250
Tth111I GACn'n_nGTC
Cuts at: 0 496 1005
Size: 496 509
XhoI C'TCGA_G
Cuts at: 0 440 1005
Size: 440 565
ZraI GAC'GTC
Cuts at: 0 563 1005
Size: 563 442
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AflIII AlfI ApaLI
ApoI AvaI BamHI BanII BclI BlpI Bme1580I BmrI
BpmI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspHI BspMI BstYI DraI EaeI EarI Eco57MI
EcoNI EcoO109I FalI HaeII HindIII MluI MscI MslI
MspA1I NspI PasI PciI PflMI PspXI PstI PvuII
SapI SfcI SmlI SphI StyI TaqII Tth111I XhoI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AgeI AhdI AleI AloI AlwNI
ApaI AscI AseI AsiSI AvrII BaeI BanI BbeI
BbsI BbvCI BcgI BciVI BfrBI BglI BglII BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI
BsiEI BsiWI BsmI BsmBI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EciI EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I
HincII HpaI KasI KpnI MfeI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TatI TstI XbaI
XcmI XmaI XmnI