Restriction map for
pFN21AE1820
(Linear) (Six-base) MAPSORT of: KIEE1820.seq Check: 6001 from: 1 to: 1002
KIEE1820.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 1002
Size: 284 718
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 539 571 1002
Size: 539 32 431
Fragments arranged by size:
539 431 32
AlwNI CAG_nnn'CTG
Cuts at: 0 262 543 681 1002
Size: 262 281 138 321
Fragments arranged by size:
321 281 262 138
ApaI G_GGCC'C
Cuts at: 0 235 806 1002
Size: 235 571 196
Fragments arranged by size:
571 235 196
ApaLI G'TGCA_C
Cuts at: 0 92 913 1002
Size: 92 821 89
Fragments arranged by size:
821 92 89
AvaI C'yCGr_G
Cuts at: 0 799 1002
Size: 799 203
BamHI G'GATC_C
Cuts at: 0 932 1002
Size: 932 70
BanI G'GyrC_C
Cuts at: 0 244 883 1002
Size: 244 639 119
Fragments arranged by size:
639 244 119
BanII G_rGCy'C
Cuts at: 0 235 278 806 827 1002
Size: 235 43 528 21 175
Fragments arranged by size:
528 235 175 43 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 290 403 1002
Size: 290 113 599
Fragments arranged by size:
599 290 113
BbvCI CC'TCA_GC
Cuts at: 0 292 1002
Size: 292 710
BglI GCCn_nnn'nGGC
Cuts at: 0 15 253 838 1002
Size: 15 238 585 164
Fragments arranged by size:
585 238 164 15
BlpI GC'TnA_GC
Cuts at: 0 856 1002
Size: 856 146
Bme1580I G_kGCm'C
Cuts at: 0 96 235 806 886 917 1002
Size: 96 139 571 80 31 85
Fragments arranged by size:
571 139 96 85 80 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 136 1002
Size: 136 866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 670 1002
Size: 497 173 332
Fragments arranged by size:
497 332 173
Bpu10I CC'TnA_GC
Cuts at: 0 292 391 1002
Size: 292 99 611
Fragments arranged by size:
611 292 99
BsaI GGTCTCn'nnnn_
Cuts at: 0 192 341 1002
Size: 192 149 661
Fragments arranged by size:
661 192 149
BsaAI yAC'GTr
Cuts at: 0 912 1002
Size: 912 90
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 231 446 667 670 1002
Size: 210 21 215 221 3 332
Fragments arranged by size:
332 221 215 210 21 3
BseYI C'CCAG_C
Cuts at: 0 13 65 97 119 270 504 536 580
Size: 13 52 32 22 151 234 32 44
Cuts at: 580 876 950 1002
Size: 296 74 52
Fragments arranged by size:
296 234 151 74 52 52 44 32
32 22 13
BsiHKAI G_wGCw'C
Cuts at: 0 96 911 917 1002
Size: 96 815 6 85
Fragments arranged by size:
815 96 85 6
Bsp1286I G_dGCh'C
Cuts at: 0 96 235 278 806 827 886 911 917
Size: 96 139 43 528 21 59 25 6
Cuts at: 917 1002
Size: 85
Fragments arranged by size:
528 139 96 85 59 43 25 21
6
BsrFI r'CCGG_y
Cuts at: 0 124 860 1002
Size: 124 736 142
Fragments arranged by size:
736 142 124
BstBI TT'CG_AA
Cuts at: 0 402 1002
Size: 402 600
BstYI r'GATC_y
Cuts at: 0 932 1002
Size: 932 70
Bsu36I CC'TnA_GG
Cuts at: 0 313 1002
Size: 313 689
BtgI C'CryG_G
Cuts at: 0 251 848 942 977 1002
Size: 251 597 94 35 25
Fragments arranged by size:
597 251 94 35 25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 193 228 1002
Size: 193 35 774
Fragments arranged by size:
774 193 35
EaeI y'GGCC_r
Cuts at: 0 52 869 890 1002
Size: 52 817 21 112
Fragments arranged by size:
817 112 52 21
EarI CTCTTCn'nnn_
Cuts at: 0 260 368 1002
Size: 260 108 634
Fragments arranged by size:
634 260 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 95 466 566 706 1002
Size: 95 371 100 140 296
Fragments arranged by size:
371 296 140 100 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 497 670 1002
Size: 284 213 173 332
Fragments arranged by size:
332 284 213 173
EcoNI CCTnn'n_nnAGG
Cuts at: 0 312 649 1002
Size: 312 337 353
Fragments arranged by size:
353 337 312
EcoO109I rG'GnC_Cy
Cuts at: 0 231 232 297 598 803 1002
Size: 231 1 65 301 205 199
Fragments arranged by size:
301 231 205 199 65 1
FspI TGC'GCA
Cuts at: 0 148 703 1002
Size: 148 555 299
Fragments arranged by size:
555 299 148
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 760 792 1002
Size: 760 32 210
Fragments arranged by size:
760 210 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 752 1002
Size: 370 382 250
Fragments arranged by size:
382 370 250
MspA1I CmG'CkG
Cuts at: 0 13 540 552 567 1002
Size: 13 527 12 15 435
Fragments arranged by size:
527 435 15 13 12
NaeI GCC'GGC
Cuts at: 0 862 1002
Size: 862 140
NcoI C'CATG_G
Cuts at: 0 251 848 1002
Size: 251 597 154
Fragments arranged by size:
597 251 154
NgoMIV G'CCGG_C
Cuts at: 0 860 1002
Size: 860 142
NspI r_CATG'y
Cuts at: 0 65 81 797 1002
Size: 65 16 716 205
Fragments arranged by size:
716 205 65 16
PasI CC'CwG_GG
Cuts at: 0 423 1002
Size: 423 579
PflMI CCAn_nnn'nTGG
Cuts at: 0 543 681 1002
Size: 543 138 321
Fragments arranged by size:
543 321 138
PfoI T'CCnGG_A
Cuts at: 0 278 1002
Size: 278 724
PmlI CAC'GTG
Cuts at: 0 912 1002
Size: 912 90
PpuMI rG'GwC_Cy
Cuts at: 0 297 598 1002
Size: 297 301 404
Fragments arranged by size:
404 301 297
PspOMI G'GGCC_C
Cuts at: 0 231 802 1002
Size: 231 571 200
Fragments arranged by size:
571 231 200
PvuII CAG'CTG
Cuts at: 0 552 567 1002
Size: 552 15 435
Fragments arranged by size:
552 435 15
SanDI GG'GwC_CC
Cuts at: 0 598 1002
Size: 598 404
SapI GCTCTTCn'nnn_
Cuts at: 0 260 368 1002
Size: 260 108 634
Fragments arranged by size:
634 260 108
SexAI A'CCwGG_T
Cuts at: 0 240 1002
Size: 240 762
SfcI C'TryA_G
Cuts at: 0 759 1002
Size: 759 243
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 838 1002
Size: 838 164
SmaI CCC'GGG
Cuts at: 0 801 1002
Size: 801 201
SphI G_CATG'C
Cuts at: 0 65 797 1002
Size: 65 732 205
Fragments arranged by size:
732 205 65
StyI C'CwwG_G
Cuts at: 0 251 848 1002
Size: 251 597 154
Fragments arranged by size:
597 251 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 111 1002
Size: 111 891
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 887 919 1002
Size: 887 32 83
Fragments arranged by size:
887 83 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 62 1002
Size: 62 940
XmaI C'CCGG_G
Cuts at: 0 799 1002
Size: 799 203
Enzymes that do cut:
AcuI AloI AlwNI ApaI ApaLI AvaI BamHI BanI
BanII BbsI BbvCI BglI BlpI Bme1580I BmrI BpmI
Bpu10I BsaI BsaAI BseRI BseYI BsiHKAI Bsp1286I BsrFI
BstBI BstYI Bsu36I BtgI CspCI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FspI Hin4I MmeI MspA1I NaeI
NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI
PspOMI PvuII SanDI SapI SexAI SfcI SfiI SmaI
SphI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BplI BpuEI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstZ17I BtgZI BtsI ClaI DraI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FalI FseI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SbfI ScaI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI