Restriction map for
pFN21AE1817
(Linear) (Six-base) MAPSORT of: KIEE1817.seq Check: 5332 from: 1 to: 996
KIEE1817.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 614 996
Size: 115 499 382
Fragments arranged by size:
499 382 115
AflIII A'CryG_T
Cuts at: 0 201 996
Size: 201 795
AlwNI CAG_nnn'CTG
Cuts at: 0 562 996
Size: 562 434
ApaI G_GGCC'C
Cuts at: 0 746 880 996
Size: 746 134 116
Fragments arranged by size:
746 134 116
ApaLI G'TGCA_C
Cuts at: 0 10 996
Size: 10 986
ApoI r'AATT_y
Cuts at: 0 607 851 996
Size: 607 244 145
Fragments arranged by size:
607 244 145
AvaI C'yCGr_G
Cuts at: 0 72 996
Size: 72 924
BamHI G'GATC_C
Cuts at: 0 655 996
Size: 655 341
BanII G_rGCy'C
Cuts at: 0 73 314 746 880 996
Size: 73 241 432 134 116
Fragments arranged by size:
432 241 134 116 73
BbvCI CC'TCA_GC
Cuts at: 0 796 996
Size: 796 200
BclI T'GATC_A
Cuts at: 0 782 996
Size: 782 214
Bme1580I G_kGCm'C
Cuts at: 0 14 463 746 880 996
Size: 14 449 283 134 116
Fragments arranged by size:
449 283 134 116 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 672 910 996
Size: 672 238 86
Fragments arranged by size:
672 238 86
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 606 996
Size: 606 390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 440 512 644 996
Size: 146 294 72 132 352
Fragments arranged by size:
352 294 146 132 72
Bpu10I CC'TnA_GC
Cuts at: 0 796 996
Size: 796 200
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 996
Size: 208 788
BsaAI yAC'GTr
Cuts at: 0 48 996
Size: 48 948
BsaWI w'CCGG_w
Cuts at: 0 33 996
Size: 33 963
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 413 443 996
Size: 413 30 553
Fragments arranged by size:
553 413 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 304 740 996
Size: 304 436 256
Fragments arranged by size:
436 304 256
BseYI C'CCAG_C
Cuts at: 0 213 996
Size: 213 783
BsiHKAI G_wGCw'C
Cuts at: 0 14 996
Size: 14 982
BsmI GAATG_Cn'
Cuts at: 0 840 996
Size: 840 156
Bsp1286I G_dGCh'C
Cuts at: 0 14 73 314 463 746 880 996
Size: 14 59 241 149 283 134 116
Fragments arranged by size:
283 241 149 134 116 59 14
BsrFI r'CCGG_y
Cuts at: 0 894 996
Size: 894 102
BstAPI GCAn_nnn'nTGC
Cuts at: 0 855 996
Size: 855 141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 371 996
Size: 371 625
BstYI r'GATC_y
Cuts at: 0 655 996
Size: 655 341
BtgI C'CryG_G
Cuts at: 0 257 869 996
Size: 257 612 127
Fragments arranged by size:
612 257 127
EaeI y'GGCC_r
Cuts at: 0 59 104 996
Size: 59 45 892
Fragments arranged by size:
892 59 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 139 668 996
Size: 139 529 328
Fragments arranged by size:
529 328 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 146 440 512 614 644 996
Size: 115 31 294 72 102 30 352
Fragments arranged by size:
352 294 115 102 72 31 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 94 346 996
Size: 94 252 650
Fragments arranged by size:
650 252 94
EcoO109I rG'GnC_Cy
Cuts at: 0 217 743 876 877 996
Size: 217 526 133 1 119
Fragments arranged by size:
526 217 133 119 1
HaeII r_GCGC'y
Cuts at: 0 113 996
Size: 113 883
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 692 719 724 751 996
Size: 692 27 5 27 245
Fragments arranged by size:
692 245 27 27 5
HincII GTy'rAC
Cuts at: 0 678 996
Size: 678 318
HindIII A'AGCT_T
Cuts at: 0 529 547 996
Size: 529 18 449
Fragments arranged by size:
529 449 18
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 996
Size: 498 498
MscI TGG'CCA
Cuts at: 0 61 996
Size: 61 935
MslI CAynn'nnrTG
Cuts at: 0 256 484 996
Size: 256 228 512
Fragments arranged by size:
512 256 228
MspA1I CmG'CkG
Cuts at: 0 390 466 514 559 996
Size: 390 76 48 45 437
Fragments arranged by size:
437 390 76 48 45
NcoI C'CATG_G
Cuts at: 0 257 996
Size: 257 739
NspI r_CATG'y
Cuts at: 0 205 996
Size: 205 791
PciI A'CATG_T
Cuts at: 0 201 996
Size: 201 795
PfoI T'CCnGG_A
Cuts at: 0 162 929 980 996
Size: 162 767 51 16
Fragments arranged by size:
767 162 51 16
PspOMI G'GGCC_C
Cuts at: 0 742 876 996
Size: 742 134 120
Fragments arranged by size:
742 134 120
PstI C_TGCA'G
Cuts at: 0 357 996
Size: 357 639
PvuII CAG'CTG
Cuts at: 0 390 466 514 559 996
Size: 390 76 48 45 437
Fragments arranged by size:
437 390 76 48 45
SfcI C'TryA_G
Cuts at: 0 353 996
Size: 353 643
SmaI CCC'GGG
Cuts at: 0 74 996
Size: 74 922
SmlI C'TyrA_G
Cuts at: 0 187 996
Size: 187 809
StuI AGG'CCT
Cuts at: 0 370 996
Size: 370 626
StyI C'CwwG_G
Cuts at: 0 257 996
Size: 257 739
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 937 996
Size: 937 59
TatI w'GTAC_w
Cuts at: 0 815 944 996
Size: 815 129 52
Fragments arranged by size:
815 129 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 413 445 996
Size: 413 32 551
Fragments arranged by size:
551 413 32
XbaI T'CTAG_A
Cuts at: 0 639 996
Size: 639 357
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 177 996
Size: 177 819
XmaI C'CCGG_G
Cuts at: 0 72 996
Size: 72 924
XmnI GAAnn'nnTTC
Cuts at: 0 838 996
Size: 838 158
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApaLI ApoI AvaI BamHI
BanII BbvCI BclI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI
Bsp1286I BsrFI BstAPI BstXI BstYI BtgI EaeI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII MmeI
MscI MslI MspA1I NcoI NspI PciI PfoI PspOMI
PstI PvuII SfcI SmaI SmlI StuI StyI TaqII
TatI TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbsI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BsaI BsaBI BsaHI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XhoI ZraI