Restriction map for
pFN21AE1815
(Linear) (Six-base) MAPSORT of: KIEE1815.seq Check: 1849 from: 1 to: 993
KIEE1815.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AccI GT'mk_AC
Cuts at: 0 234 668 993
Size: 234 434 325
Fragments arranged by size:
434 325 234
Acc65I G'GTAC_C
Cuts at: 0 24 993
Size: 24 969
AleI CACnn'nnGTG
Cuts at: 0 205 993
Size: 205 788
AlwNI CAG_nnn'CTG
Cuts at: 0 34 510 573 993
Size: 34 476 63 420
Fragments arranged by size:
476 420 63 34
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 660 693 993
Size: 660 33 300
Fragments arranged by size:
660 300 33
BamHI G'GATC_C
Cuts at: 0 984 993
Size: 984 9
BanI G'GyrC_C
Cuts at: 0 24 199 993
Size: 24 175 794
Fragments arranged by size:
794 175 24
BanII G_rGCy'C
Cuts at: 0 878 993
Size: 878 115
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 803 847 993
Size: 125 678 44 146
Fragments arranged by size:
678 146 125 44
BglII A'GATC_T
Cuts at: 0 399 993
Size: 399 594
BlpI GC'TnA_GC
Cuts at: 0 757 993
Size: 757 236
BmrI ACTGGGnnnn_n'
Cuts at: 0 837 993
Size: 837 156
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 711 993
Size: 711 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 571 734 993
Size: 421 150 163 259
Fragments arranged by size:
421 259 163 150
Bpu10I CC'TnA_GC
Cuts at: 0 473 968 993
Size: 473 495 25
Fragments arranged by size:
495 473 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 682 993
Size: 682 311
BsaHI Gr'CG_yC
Cuts at: 0 17 993
Size: 17 976
BsaWI w'CCGG_w
Cuts at: 0 44 917 993
Size: 44 873 76
Fragments arranged by size:
873 76 44
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 141 442 667 684 993
Size: 141 301 225 17 309
Fragments arranged by size:
309 301 225 141 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 993
Size: 713 280
BsiHKAI G_wGCw'C
Cuts at: 0 878 993
Size: 878 115
BsmI GAATG_Cn'
Cuts at: 0 909 993
Size: 909 84
Bsp1286I G_dGCh'C
Cuts at: 0 878 993
Size: 878 115
BspEI T'CCGG_A
Cuts at: 0 44 917 993
Size: 44 873 76
Fragments arranged by size:
873 76 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 965 993
Size: 965 28
BsrBI CCG'CTC
Cuts at: 0 870 993
Size: 870 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 573 906 993
Size: 573 333 87
Fragments arranged by size:
573 333 87
BstEII G'GTnAC_C
Cuts at: 0 6 786 952 993
Size: 6 780 166 41
Fragments arranged by size:
780 166 41 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 810 993
Size: 810 183
BstYI r'GATC_y
Cuts at: 0 41 399 984 993
Size: 41 358 585 9
Fragments arranged by size:
585 358 41 9
Bsu36I CC'TnA_GG
Cuts at: 0 124 993
Size: 124 869
DraIII CAC_nnn'GTG
Cuts at: 0 99 952 993
Size: 99 853 41
Fragments arranged by size:
853 99 41
EaeI y'GGCC_r
Cuts at: 0 101 149 380 993
Size: 101 48 231 613
Fragments arranged by size:
613 231 101 48
EarI CTCTTCn'nnn_
Cuts at: 0 623 993
Size: 623 370
EcoICRI GAG'CTC
Cuts at: 0 876 993
Size: 876 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 571 734 993
Size: 421 150 163 259
Fragments arranged by size:
421 259 163 150
EcoNI CCTnn'n_nnAGG
Cuts at: 0 190 424 472 993
Size: 190 234 48 521
Fragments arranged by size:
521 234 190 48
EcoO109I rG'GnC_Cy
Cuts at: 0 432 993
Size: 432 561
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 33 65 993
Size: 33 32 928
Fragments arranged by size:
928 33 32
HincII GTy'rAC
Cuts at: 0 450 993
Size: 450 543
KpnI G_GTAC'C
Cuts at: 0 28 993
Size: 28 965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 993
Size: 549 444
MscI TGG'CCA
Cuts at: 0 151 382 993
Size: 151 231 611
Fragments arranged by size:
611 231 151
MslI CAynn'nnrTG
Cuts at: 0 205 993
Size: 205 788
MspA1I CmG'CkG
Cuts at: 0 353 390 507 579 993
Size: 353 37 117 72 414
Fragments arranged by size:
414 353 117 72 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 34 594 993
Size: 34 560 399
Fragments arranged by size:
560 399 34
PpuMI rG'GwC_Cy
Cuts at: 0 432 993
Size: 432 561
PshAI GACnn'nnGTC
Cuts at: 0 172 993
Size: 172 821
PstI C_TGCA'G
Cuts at: 0 515 578 893 962 993
Size: 515 63 315 69 31
Fragments arranged by size:
515 315 69 63 31
PvuII CAG'CTG
Cuts at: 0 390 579 993
Size: 390 189 414
Fragments arranged by size:
414 390 189
SacI G_AGCT'C
Cuts at: 0 878 993
Size: 878 115
SexAI A'CCwGG_T
Cuts at: 0 948 993
Size: 948 45
SfcI C'TryA_G
Cuts at: 0 78 511 574 889 958 993
Size: 78 433 63 315 69 35
Fragments arranged by size:
433 315 78 69 63 35
StuI AGG'CCT
Cuts at: 0 136 862 940 993
Size: 136 726 78 53
Fragments arranged by size:
726 136 78 53
StyI C'CwwG_G
Cuts at: 0 791 993
Size: 791 202
Enzymes that do cut:
AccI Acc65I AleI AlwNI BaeI BamHI BanI BanII
BbsI BglII BlpI BmrI BplI BpmI Bpu10I BsaI
BsaHI BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BstAPI BstEII BstXI BstYI Bsu36I DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII
KpnI MmeI MscI MslI MspA1I PflMI PpuMI PshAI
PstI PvuII SacI SexAI SfcI StuI StyI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI Bme1580I BmgBI BmtI BpuEI BsaAI BsaBI
BsaXI BseYI BsiEI BsiWI BsmBI BspHI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI