Restriction map for
pFN21AE1814
(Linear) (Six-base) MAPSORT of: KIEE1814.seq Check: 4509 from: 1 to: 987
KIEE1814.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AatII G_ACGT'C
Cuts at: 0 13 987
Size: 13 974
AccI GT'mk_AC
Cuts at: 0 32 987
Size: 32 955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 987
Size: 460 527
AflIII A'CryG_T
Cuts at: 0 432 902 987
Size: 432 470 85
Fragments arranged by size:
470 432 85
AgeI A'CCGG_T
Cuts at: 0 364 987
Size: 364 623
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 652 712 987
Size: 652 60 275
Fragments arranged by size:
652 275 60
AlwNI CAG_nnn'CTG
Cuts at: 0 174 918 987
Size: 174 744 69
Fragments arranged by size:
744 174 69
ApoI r'AATT_y
Cuts at: 0 103 987
Size: 103 884
AvaI C'yCGr_G
Cuts at: 0 604 987
Size: 604 383
BanII G_rGCy'C
Cuts at: 0 354 727 987
Size: 354 373 260
Fragments arranged by size:
373 354 260
BbsI GAAGACnn'nnnn_
Cuts at: 0 507 968 987
Size: 507 461 19
Fragments arranged by size:
507 461 19
BlpI GC'TnA_GC
Cuts at: 0 175 987
Size: 175 812
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 786 987
Size: 786 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 507 987
Size: 183 324 480
Fragments arranged by size:
480 324 183
Bpu10I CC'TnA_GC
Cuts at: 0 625 721 987
Size: 625 96 266
Fragments arranged by size:
625 266 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 373 987
Size: 373 614
BsaAI yAC'GTr
Cuts at: 0 210 987
Size: 210 777
BsaHI Gr'CG_yC
Cuts at: 0 10 987
Size: 10 977
BsaWI w'CCGG_w
Cuts at: 0 364 987
Size: 364 623
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 709 739 987
Size: 709 30 248
Fragments arranged by size:
709 248 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 484 987
Size: 394 90 503
Fragments arranged by size:
503 394 90
BseYI C'CCAG_C
Cuts at: 0 278 398 911 987
Size: 278 120 513 76
Fragments arranged by size:
513 278 120 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 816 987
Size: 167 649 171
Fragments arranged by size:
649 171 167
BsiHKAI G_wGCw'C
Cuts at: 0 69 727 987
Size: 69 658 260
Fragments arranged by size:
658 260 69
Bsp1286I G_dGCh'C
Cuts at: 0 69 354 727 987
Size: 69 285 373 260
Fragments arranged by size:
373 285 260 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 233 501 987
Size: 233 268 486
Fragments arranged by size:
486 268 233
BsrDI GCAATG_nn'
Cuts at: 0 422 987
Size: 422 565
BsrFI r'CCGG_y
Cuts at: 0 364 987
Size: 364 623
BstXI CCAn_nnnn'nTGG
Cuts at: 0 161 676 987
Size: 161 515 311
Fragments arranged by size:
515 311 161
BstYI r'GATC_y
Cuts at: 0 260 866 987
Size: 260 606 121
Fragments arranged by size:
606 260 121
BtsI GCAGTG_nn'
Cuts at: 0 287 987
Size: 287 700
DraIII CAC_nnn'GTG
Cuts at: 0 213 987
Size: 213 774
EaeI y'GGCC_r
Cuts at: 0 44 204 461 857 920 987
Size: 44 160 257 396 63 67
Fragments arranged by size:
396 257 160 67 63 44
EarI CTCTTCn'nnn_
Cuts at: 0 118 835 987
Size: 118 717 152
Fragments arranged by size:
717 152 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 859 987
Size: 859 128
EcoICRI GAG'CTC
Cuts at: 0 725 987
Size: 725 262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 460 507 987
Size: 183 277 47 480
Fragments arranged by size:
480 277 183 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 46 987
Size: 46 941
EcoO109I rG'GnC_Cy
Cuts at: 0 717 987
Size: 717 270
EcoRI G'AATT_C
Cuts at: 0 103 987
Size: 103 884
HaeII r_GCGC'y
Cuts at: 0 77 987
Size: 77 910
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 709 741 987
Size: 709 32 246
Fragments arranged by size:
709 246 32
HincII GTy'rAC
Cuts at: 0 138 987
Size: 138 849
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 987
Size: 132 855
MscI TGG'CCA
Cuts at: 0 46 463 859 987
Size: 46 417 396 128
Fragments arranged by size:
417 396 128 46
MslI CAynn'nnrTG
Cuts at: 0 694 987
Size: 694 293
MspA1I CmG'CkG
Cuts at: 0 161 282 507 672 855 987
Size: 161 121 225 165 183 132
Fragments arranged by size:
225 183 165 161 132 121
NspI r_CATG'y
Cuts at: 0 436 987
Size: 436 551
PciI A'CATG_T
Cuts at: 0 432 987
Size: 432 555
PflMI CCAn_nnn'nTGG
Cuts at: 0 213 918 987
Size: 213 705 69
Fragments arranged by size:
705 213 69
PfoI T'CCnGG_A
Cuts at: 0 617 987
Size: 617 370
PmlI CAC'GTG
Cuts at: 0 210 987
Size: 210 777
PpuMI rG'GwC_Cy
Cuts at: 0 717 987
Size: 717 270
PshAI GACnn'nnGTC
Cuts at: 0 9 987
Size: 9 978
PstI C_TGCA'G
Cuts at: 0 230 512 987
Size: 230 282 475
Fragments arranged by size:
475 282 230
PvuII CAG'CTG
Cuts at: 0 161 282 507 672 855 987
Size: 161 121 225 165 183 132
Fragments arranged by size:
225 183 165 161 132 121
RsrII CG'GwC_CG
Cuts at: 0 738 987
Size: 738 249
SacI G_AGCT'C
Cuts at: 0 727 987
Size: 727 260
SbfI CC_TGCA'GG
Cuts at: 0 230 987
Size: 230 757
SexAI A'CCwGG_T
Cuts at: 0 246 987
Size: 246 741
SfcI C'TryA_G
Cuts at: 0 226 498 508 987
Size: 226 272 10 479
Fragments arranged by size:
479 272 226 10
StuI AGG'CCT
Cuts at: 0 574 829 987
Size: 574 255 158
Fragments arranged by size:
574 255 158
StyI C'CwwG_G
Cuts at: 0 47 117 195 293 860 987
Size: 47 70 78 98 567 127
Fragments arranged by size:
567 127 98 78 70 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 807 987
Size: 292 515 180
Fragments arranged by size:
515 292 180
TatI w'GTAC_w
Cuts at: 0 776 782 987
Size: 776 6 205
Fragments arranged by size:
776 205 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 894 987
Size: 894 93
ZraI GAC'GTC
Cuts at: 0 11 987
Size: 11 976
Enzymes that do cut:
AatII AccI AcuI AflIII AgeI AlfI AlwNI ApoI
AvaI BanII BbsI BlpI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BstXI BstYI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NspI
PciI PflMI PfoI PmlI PpuMI PshAI PstI PvuII
RsrII SacI SbfI SexAI SfcI StuI StyI TaqII
TatI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII Bme1580I BmgBI BmrI BmtI BpuEI BsaBI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DrdI EagI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PpiI PsiI PspOMI PspXI
PsrI PvuI SacII SalI SanDI SapI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI