Restriction map for
pFN21AE1813
(Linear) (Six-base) MAPSORT of: KIEE1813.seq Check: 932 from: 1 to: 987
KIEE1813.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AccI GT'mk_AC
Cuts at: 0 356 987
Size: 356 631
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 611 724 987
Size: 181 430 113 263
Fragments arranged by size:
430 263 181 113
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 675 987
Size: 675 312
AlwNI CAG_nnn'CTG
Cuts at: 0 352 987
Size: 352 635
AvaI C'yCGr_G
Cuts at: 0 682 719 987
Size: 682 37 268
Fragments arranged by size:
682 268 37
BanI G'GyrC_C
Cuts at: 0 436 987
Size: 436 551
BanII G_rGCy'C
Cuts at: 0 11 345 987
Size: 11 334 642
Fragments arranged by size:
642 334 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 97 987
Size: 97 890
BglI GCCn_nnn'nGGC
Cuts at: 0 967 987
Size: 967 20
Bme1580I G_kGCm'C
Cuts at: 0 441 987
Size: 441 546
BmrI ACTGGGnnnn_n'
Cuts at: 0 84 912 987
Size: 84 828 75
Fragments arranged by size:
828 84 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 59 987
Size: 59 928
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 987
Size: 463 524
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 439 987
Size: 205 234 548
Fragments arranged by size:
548 234 205
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 987
Size: 70 917
BseYI C'CCAG_C
Cuts at: 0 890 987
Size: 890 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 987
Size: 586 401
Bsp1286I G_dGCh'C
Cuts at: 0 11 345 441 987
Size: 11 334 96 546
Fragments arranged by size:
546 334 96 11
BsrDI GCAATG_nn'
Cuts at: 0 956 987
Size: 956 31
BsrGI T'GTAC_A
Cuts at: 0 632 987
Size: 632 355
BstYI r'GATC_y
Cuts at: 0 677 987
Size: 677 310
Bsu36I CC'TnA_GG
Cuts at: 0 53 987
Size: 53 934
BtsI GCAGTG_nn'
Cuts at: 0 719 845 987
Size: 719 126 142
Fragments arranged by size:
719 142 126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 933 968 987
Size: 933 35 19
Fragments arranged by size:
933 35 19
EaeI y'GGCC_r
Cuts at: 0 740 968 987
Size: 740 228 19
Fragments arranged by size:
740 228 19
EarI CTCTTCn'nnn_
Cuts at: 0 245 614 700 987
Size: 245 369 86 287
Fragments arranged by size:
369 287 245 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 463 611 724 987
Size: 181 282 148 113 263
Fragments arranged by size:
282 263 181 148 113
EcoO109I rG'GnC_Cy
Cuts at: 0 727 987
Size: 727 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 496 528 836 868 908 940
Size: 115 32 349 32 308 32 40 32
Cuts at: 940 987
Size: 47
Fragments arranged by size:
349 308 115 47 40 32 32 32
32
HincII GTy'rAC
Cuts at: 0 357 987
Size: 357 630
MscI TGG'CCA
Cuts at: 0 742 970 987
Size: 742 228 17
Fragments arranged by size:
742 228 17
MslI CAynn'nnrTG
Cuts at: 0 209 987
Size: 209 778
MspA1I CmG'CkG
Cuts at: 0 890 987
Size: 890 97
NdeI CA'TA_TG
Cuts at: 0 210 606 987
Size: 210 396 381
Fragments arranged by size:
396 381 210
PpuMI rG'GwC_Cy
Cuts at: 0 727 987
Size: 727 260
PvuII CAG'CTG
Cuts at: 0 890 987
Size: 890 97
SalI G'TCGA_C
Cuts at: 0 355 987
Size: 355 632
SmlI C'TyrA_G
Cuts at: 0 10 184 454 987
Size: 10 174 270 533
Fragments arranged by size:
533 270 174 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 424 987
Size: 85 339 563
Fragments arranged by size:
563 339 85
TatI w'GTAC_w
Cuts at: 0 632 987
Size: 632 355
Tth111I GACn'n_nGTC
Cuts at: 0 352 987
Size: 352 635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 987
Size: 252 735
Enzymes that do cut:
AccI AcuI AlfI AlwNI AvaI BanI BanII BbsI
BglI Bme1580I BmrI BplI BpmI BpuEI BseRI BseYI
BsgI Bsp1286I BsrDI BsrGI BstYI Bsu36I BtsI CspCI
EaeI EarI Eco57MI EcoO109I Hin4I HincII MscI MslI
MspA1I NdeI PpuMI PvuII SalI SmlI TaqII TatI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI
ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TstI XbaI XhoI XmaI
XmnI ZraI