Restriction map for
pFN21AE1811
(Linear) (Six-base) MAPSORT of: KIEE1811.seq Check: 3266 from: 1 to: 981
KIEE1811.orf
With 182 enzymes: SgfI *
July 15, 2010 18:48 ..
AatII G_ACGT'C
Cuts at: 0 833 981
Size: 833 148
AccI GT'mk_AC
Cuts at: 0 977 981
Size: 977 4
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 981
Size: 700 281
AhdI GACnn_n'nnGTC
Cuts at: 0 100 981
Size: 100 881
ApoI r'AATT_y
Cuts at: 0 67 225 981
Size: 67 158 756
Fragments arranged by size:
756 158 67
BamHI G'GATC_C
Cuts at: 0 923 981
Size: 923 58
BanI G'GyrC_C
Cuts at: 0 889 981
Size: 889 92
BbsI GAAGACnn'nnnn_
Cuts at: 0 96 263 981
Size: 96 167 718
Fragments arranged by size:
718 167 96
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 573 930 981
Size: 573 357 51
Fragments arranged by size:
573 357 51
Bpu10I CC'TnA_GC
Cuts at: 0 341 800 981
Size: 341 459 181
Fragments arranged by size:
459 341 181
BsaI GGTCTCn'nnnn_
Cuts at: 0 339 559 981
Size: 339 220 422
Fragments arranged by size:
422 339 220
BsaAI yAC'GTr
Cuts at: 0 579 981
Size: 579 402
BsaHI Gr'CG_yC
Cuts at: 0 830 981
Size: 830 151
BsiHKAI G_wGCw'C
Cuts at: 0 115 322 981
Size: 115 207 659
Fragments arranged by size:
659 207 115
BsmI GAATG_Cn'
Cuts at: 0 283 981
Size: 283 698
Bsp1286I G_dGCh'C
Cuts at: 0 115 322 981
Size: 115 207 659
Fragments arranged by size:
659 207 115
BsrDI GCAATG_nn'
Cuts at: 0 455 981
Size: 455 526
BstYI r'GATC_y
Cuts at: 0 923 981
Size: 923 58
BtgI C'CryG_G
Cuts at: 0 919 981
Size: 919 62
BtsI GCAGTG_nn'
Cuts at: 0 391 421 981
Size: 391 30 560
Fragments arranged by size:
560 391 30
DrdI GACnn_nn'nnGTC
Cuts at: 0 207 445 981
Size: 207 238 536
Fragments arranged by size:
536 238 207
EaeI y'GGCC_r
Cuts at: 0 490 836 981
Size: 490 346 145
Fragments arranged by size:
490 346 145
EarI CTCTTCn'nnn_
Cuts at: 0 130 981
Size: 130 851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 981
Size: 700 281
EcoO109I rG'GnC_Cy
Cuts at: 0 168 756 876 981
Size: 168 588 120 105
Fragments arranged by size:
588 168 120 105
EcoRI G'AATT_C
Cuts at: 0 67 981
Size: 67 914
EcoRV GAT'ATC
Cuts at: 0 962 981
Size: 962 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 86 118 706 738 981
Size: 86 32 588 32 243
Fragments arranged by size:
588 243 86 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 647 981
Size: 338 309 334
Fragments arranged by size:
338 334 309
MscI TGG'CCA
Cuts at: 0 492 981
Size: 492 489
NcoI C'CATG_G
Cuts at: 0 919 981
Size: 919 62
PasI CC'CwG_GG
Cuts at: 0 776 981
Size: 776 205
PfoI T'CCnGG_A
Cuts at: 0 710 981
Size: 710 271
PmlI CAC'GTG
Cuts at: 0 579 981
Size: 579 402
PpuMI rG'GwC_Cy
Cuts at: 0 756 876 981
Size: 756 120 105
Fragments arranged by size:
756 120 105
SexAI A'CCwGG_T
Cuts at: 0 752 981
Size: 752 229
SfcI C'TryA_G
Cuts at: 0 584 981
Size: 584 397
SspI AAT'ATT
Cuts at: 0 652 981
Size: 652 329
StyI C'CwwG_G
Cuts at: 0 255 329 570 919 981
Size: 255 74 241 349 62
Fragments arranged by size:
349 255 241 74 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 5 754 981
Size: 5 749 227
Fragments arranged by size:
749 227 5
Tth111I GACn'n_nGTC
Cuts at: 0 754 981
Size: 754 227
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 712 934 981
Size: 712 222 47
Fragments arranged by size:
712 222 47
XmnI GAAnn'nnTTC
Cuts at: 0 283 981
Size: 283 698
ZraI GAC'GTC
Cuts at: 0 831 981
Size: 831 150
Enzymes that do cut:
AatII AccI AcuI AhdI ApoI BamHI BanI BbsI
BplI Bpu10I BsaI BsaAI BsaHI BsiHKAI BsmI Bsp1286I
BsrDI BstYI BtgI BtsI DrdI EaeI EarI Eco57MI
EcoO109I EcoRI EcoRV Hin4I MmeI MscI NcoI PasI
PfoI PmlI PpuMI SexAI SfcI SspI StyI TaqII
Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoNI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TatI TstI XbaI XhoI XmaI