Restriction map for
pFN21AE1809
(Linear) (Six-base) MAPSORT of: KIEE1809.seq Check: 8553 from: 1 to: 975
KIEE1809.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 975
Size: 875 100
AfeI AGC'GCT
Cuts at: 0 385 975
Size: 385 590
AflIII A'CryG_T
Cuts at: 0 5 975
Size: 5 970
AleI CACnn'nnGTG
Cuts at: 0 229 975
Size: 229 746
AlwNI CAG_nnn'CTG
Cuts at: 0 180 738 975
Size: 180 558 237
Fragments arranged by size:
558 237 180
ApoI r'AATT_y
Cuts at: 0 783 975
Size: 783 192
AvaI C'yCGr_G
Cuts at: 0 134 524 927 975
Size: 134 390 403 48
Fragments arranged by size:
403 390 134 48
BanI G'GyrC_C
Cuts at: 0 61 806 975
Size: 61 745 169
Fragments arranged by size:
745 169 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 863 975
Size: 863 112
BbvCI CC'TCA_GC
Cuts at: 0 502 975
Size: 502 473
BglI GCCn_nnn'nGGC
Cuts at: 0 519 975
Size: 519 456
BglII A'GATC_T
Cuts at: 0 374 975
Size: 374 601
Bme1580I G_kGCm'C
Cuts at: 0 308 975
Size: 308 667
BmgBI CAC'GTC
Cuts at: 0 531 975
Size: 531 444
BmrI ACTGGGnnnn_n'
Cuts at: 0 108 874 975
Size: 108 766 101
Fragments arranged by size:
766 108 101
BmtI G_CTAG'C
Cuts at: 0 322 975
Size: 322 653
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 909 975
Size: 909 66
Bpu10I CC'TnA_GC
Cuts at: 0 502 975
Size: 502 473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 975
Size: 547 428
BsaI GGTCTCn'nnnn_
Cuts at: 0 256 975
Size: 256 719
BsaAI yAC'GTr
Cuts at: 0 315 597 765 975
Size: 315 282 168 210
Fragments arranged by size:
315 282 210 168
BsaBI GATnn'nnATC
Cuts at: 0 725 975
Size: 725 250
BseYI C'CCAG_C
Cuts at: 0 397 778 975
Size: 397 381 197
Fragments arranged by size:
397 381 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 206 400 975
Size: 193 13 194 575
Fragments arranged by size:
575 194 193 13
BsiHKAI G_wGCw'C
Cuts at: 0 641 975
Size: 641 334
BsmBI CGTCTCn'nnnn_
Cuts at: 0 521 975
Size: 521 454
Bsp1286I G_dGCh'C
Cuts at: 0 308 641 975
Size: 308 333 334
Fragments arranged by size:
334 333 308
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 746 975
Size: 746 229
BsrBI CCG'CTC
Cuts at: 0 290 975
Size: 290 685
BsrDI GCAATG_nn'
Cuts at: 0 351 975
Size: 351 624
BstAPI GCAn_nnn'nTGC
Cuts at: 0 738 975
Size: 738 237
BstYI r'GATC_y
Cuts at: 0 176 374 425 975
Size: 176 198 51 550
Fragments arranged by size:
550 198 176 51
BtgI C'CryG_G
Cuts at: 0 89 975
Size: 89 886
BtsI GCAGTG_nn'
Cuts at: 0 651 676 698 975
Size: 651 25 22 277
Fragments arranged by size:
651 277 25 22
DraIII CAC_nnn'GTG
Cuts at: 0 481 868 975
Size: 481 387 107
Fragments arranged by size:
481 387 107
DrdI GACnn_nn'nnGTC
Cuts at: 0 441 975
Size: 441 534
EaeI y'GGCC_r
Cuts at: 0 143 449 581 609 673 683 714 975
Size: 143 306 132 28 64 10 31 261
Fragments arranged by size:
306 261 143 132 64 31 28 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 975
Size: 875 100
EcoO109I rG'GnC_Cy
Cuts at: 0 108 521 819 975
Size: 108 413 298 156
Fragments arranged by size:
413 298 156 108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 957 975
Size: 957 18
HaeII r_GCGC'y
Cuts at: 0 19 387 975
Size: 19 368 588
Fragments arranged by size:
588 368 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 975
Size: 265 32 678
Fragments arranged by size:
678 265 32
MscI TGG'CCA
Cuts at: 0 145 451 611 685 975
Size: 145 306 160 74 290
Fragments arranged by size:
306 290 160 145 74
MslI CAynn'nnrTG
Cuts at: 0 229 457 975
Size: 229 228 518
Fragments arranged by size:
518 229 228
MspA1I CmG'CkG
Cuts at: 0 552 573 975
Size: 552 21 402
Fragments arranged by size:
552 402 21
NheI G'CTAG_C
Cuts at: 0 318 975
Size: 318 657
NspI r_CATG'y
Cuts at: 0 9 975
Size: 9 966
PciI A'CATG_T
Cuts at: 0 5 975
Size: 5 970
PflMI CCAn_nnn'nTGG
Cuts at: 0 386 657 975
Size: 386 271 318
Fragments arranged by size:
386 318 271
PfoI T'CCnGG_A
Cuts at: 0 257 975
Size: 257 718
PpuMI rG'GwC_Cy
Cuts at: 0 108 819 975
Size: 108 711 156
Fragments arranged by size:
711 156 108
PstI C_TGCA'G
Cuts at: 0 734 975
Size: 734 241
PvuII CAG'CTG
Cuts at: 0 552 573 975
Size: 552 21 402
Fragments arranged by size:
552 402 21
SanDI GG'GwC_CC
Cuts at: 0 108 975
Size: 108 867
SfcI C'TryA_G
Cuts at: 0 730 975
Size: 730 245
SmaI CCC'GGG
Cuts at: 0 136 975
Size: 136 839
SmlI C'TyrA_G
Cuts at: 0 562 975
Size: 562 413
StuI AGG'CCT
Cuts at: 0 925 975
Size: 925 50
StyI C'CwwG_G
Cuts at: 0 152 339 975
Size: 152 187 636
Fragments arranged by size:
636 187 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 172 856 975
Size: 82 90 684 119
Fragments arranged by size:
684 119 90 82
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 337 444 459 975
Size: 337 107 15 516
Fragments arranged by size:
516 337 107 15
XmaI C'CCGG_G
Cuts at: 0 134 975
Size: 134 841
Enzymes that do cut:
AcuI AfeI AflIII AleI AlwNI ApoI AvaI BanI
BbsI BbvCI BglI BglII Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BstAPI BstYI
BtgI BtsI DraIII DrdI EaeI Eco57MI EcoO109I FalI
HaeII Hin4I MscI MslI MspA1I NheI NspI PciI
PflMI PfoI PpuMI PstI PvuII SanDI SfcI SmaI
SmlI StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbeI BcgI BciVI BclI BfrBI
BlpI BpmI BsaHI BsaWI BsaXI BseRI BsiEI BsiWI
BsmI BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI XhoI XmnI ZraI