(Linear) (Six-base) MAPSORT of: KIEE1807.seq  Check: 436  from: 1  to: 969



KIEE1807.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:48  ..

AccI GT'mk_AC

Cuts at:      0     404     969 
   Size:        404     565
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     969 
   Size:         92     877
 
ApoI r'AATT_y

Cuts at:      0     586     969 
   Size:        586     383
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     587     716     969 
   Size:        587     129     253

  Fragments arranged by size: 

                 587     253     129
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     893     927     969 
   Size:        893      34      42

  Fragments arranged by size: 

                 893      42      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     909     969 
   Size:        909      60
 
Bme1580I G_kGCm'C

Cuts at:      0     574     969 
   Size:        574     395
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     635     969 
   Size:        635     334
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     944     969 
   Size:        944      25
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     371     969 
   Size:        371     598
 
BsaBI GATnn'nnATC

Cuts at:      0     343     515     969 
   Size:        343     172     454

  Fragments arranged by size: 

                 454     343     172
 
BsaWI w'CCGG_w

Cuts at:      0     455     969 
   Size:        455     514
 
BsmI GAATG_Cn'

Cuts at:      0     128     969 
   Size:        128     841
 
Bsp1286I G_dGCh'C

Cuts at:      0     574     969 
   Size:        574     395
 
BspEI T'CCGG_A

Cuts at:      0     455     969 
   Size:        455     514
 
BsrFI r'CCGG_y

Cuts at:      0     750     969 
   Size:        750     219
 
BstBI TT'CG_AA

Cuts at:      0     861     969 
   Size:        861     108
 
BstYI r'GATC_y

Cuts at:      0     596     969 
   Size:        596     373
 
BstZ17I GTA'TAC

Cuts at:      0     405     969 
   Size:        405     564
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     141     969 
   Size:        141     828
 
EaeI y'GGCC_r

Cuts at:      0     231     521     722     969 
   Size:        231     290     201     247

  Fragments arranged by size: 

                 290     247     231     201
 
EarI CTCTTCn'nnn_

Cuts at:      0     406     483     969 
   Size:        406      77     486

  Fragments arranged by size: 

                 486     406      77
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     944     969 
   Size:         92     852      25

  Fragments arranged by size: 

                 852      92      25
 
EcoRV GAT'ATC

Cuts at:      0     381     969 
   Size:        381     588
 
HincII GTy'rAC

Cuts at:      0     470     969 
   Size:        470     499
 
MscI TGG'CCA

Cuts at:      0     233     523     724     969 
   Size:        233     290     201     245

  Fragments arranged by size: 

                 290     245     233     201
 
MspA1I CmG'CkG

Cuts at:      0     156     162     969 
   Size:        156       6     807

  Fragments arranged by size: 

                 807     156       6
 
NaeI GCC'GGC

Cuts at:      0     752     969 
   Size:        752     217
 
NdeI CA'TA_TG

Cuts at:      0     189     969 
   Size:        189     780
 
NgoMIV G'CCGG_C

Cuts at:      0     750     969 
   Size:        750     219
 
PvuII CAG'CTG

Cuts at:      0     162     969 
   Size:        162     807
 
SmlI C'TyrA_G

Cuts at:      0     386     969 
   Size:        386     583
 
XmnI GAAnn'nnTTC

Cuts at:      0      77     969 
   Size:         77     892
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BbsI     BcgI    BciVI Bme1580I     BplI
     BpmI    BpuEI    BsaBI    BsaWI     BsmI Bsp1286I    BspEI    BsrFI
    BstBI    BstYI  BstZ17I     DrdI     EaeI     EarI  Eco57MI    EcoRV
   HincII     MscI   MspA1I     NaeI     NdeI   NgoMIV    PvuII     SmlI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI     NarI     NcoI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI