Restriction map for
pFN21AE1791
(Linear) (Six-base) MAPSORT of: KIEE1791.seq Check: 559 from: 1 to: 909
KIEE1791.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 308 909
Size: 308 601
AlwNI CAG_nnn'CTG
Cuts at: 0 79 607 909
Size: 79 528 302
Fragments arranged by size:
528 302 79
BanII G_rGCy'C
Cuts at: 0 400 874 909
Size: 400 474 35
Fragments arranged by size:
474 400 35
BmgBI CAC'GTC
Cuts at: 0 198 909
Size: 198 711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 519 601 807 909
Size: 231 288 82 206 102
Fragments arranged by size:
288 231 206 102 82
Bpu10I CC'TnA_GC
Cuts at: 0 399 909
Size: 399 510
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 909
Size: 850 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 877 909
Size: 877 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 492 522 909
Size: 492 30 387
Fragments arranged by size:
492 387 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 640 675 742 909
Size: 640 35 67 167
Fragments arranged by size:
640 167 67 35
BseYI C'CCAG_C
Cuts at: 0 478 699 850 909
Size: 478 221 151 59
Fragments arranged by size:
478 221 151 59
BsiHKAI G_wGCw'C
Cuts at: 0 400 620 846 909
Size: 400 220 226 63
Fragments arranged by size:
400 226 220 63
BsmBI CGTCTCn'nnnn_
Cuts at: 0 207 909
Size: 207 702
Bsp1286I G_dGCh'C
Cuts at: 0 400 620 846 874 909
Size: 400 220 226 28 35
Fragments arranged by size:
400 226 220 35 28
BspHI T'CATG_A
Cuts at: 0 626 909
Size: 626 283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 350 909
Size: 350 559
BsrBI CCG'CTC
Cuts at: 0 320 909
Size: 320 589
BsrFI r'CCGG_y
Cuts at: 0 5 284 526 909
Size: 5 279 242 383
Fragments arranged by size:
383 279 242 5
BstYI r'GATC_y
Cuts at: 0 76 332 768 909
Size: 76 256 436 141
Fragments arranged by size:
436 256 141 76
Bsu36I CC'TnA_GG
Cuts at: 0 328 532 652 909
Size: 328 204 120 257
Fragments arranged by size:
328 257 204 120
BtgI C'CryG_G
Cuts at: 0 131 228 909
Size: 131 97 681
Fragments arranged by size:
681 131 97
BtsI GCAGTG_nn'
Cuts at: 0 418 608 909
Size: 418 190 301
Fragments arranged by size:
418 301 190
DrdI GACnn_nn'nnGTC
Cuts at: 0 799 909
Size: 799 110
EarI CTCTTCn'nnn_
Cuts at: 0 310 909
Size: 310 599
EcoICRI GAG'CTC
Cuts at: 0 398 909
Size: 398 511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 519 601 807 909
Size: 231 288 82 206 102
Fragments arranged by size:
288 231 206 102 82
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 275 339 909
Size: 275 64 570
Fragments arranged by size:
570 275 64
FseI GG_CCGG'CC
Cuts at: 0 9 909
Size: 9 900
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 294 326 909
Size: 294 32 583
Fragments arranged by size:
583 294 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 909
Size: 362 547
MspA1I CmG'CkG
Cuts at: 0 681 909
Size: 681 228
NaeI GCC'GGC
Cuts at: 0 7 909
Size: 7 902
NcoI C'CATG_G
Cuts at: 0 131 909
Size: 131 778
NdeI CA'TA_TG
Cuts at: 0 735 909
Size: 735 174
NgoMIV G'CCGG_C
Cuts at: 0 5 909
Size: 5 904
PasI CC'CwG_GG
Cuts at: 0 173 909
Size: 173 736
PfoI T'CCnGG_A
Cuts at: 0 208 909
Size: 208 701
PstI C_TGCA'G
Cuts at: 0 59 347 909
Size: 59 288 562
Fragments arranged by size:
562 288 59
SacI G_AGCT'C
Cuts at: 0 400 909
Size: 400 509
SapI GCTCTTCn'nnn_
Cuts at: 0 310 909
Size: 310 599
SfcI C'TryA_G
Cuts at: 0 55 243 343 909
Size: 55 188 100 566
Fragments arranged by size:
566 188 100 55
SmlI C'TyrA_G
Cuts at: 0 865 909
Size: 865 44
StyI C'CwwG_G
Cuts at: 0 131 785 909
Size: 131 654 124
Fragments arranged by size:
654 131 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 124 463 484 852 909
Size: 124 339 21 368 57
Fragments arranged by size:
368 339 124 57 21
TatI w'GTAC_w
Cuts at: 0 890 909
Size: 890 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 909
Size: 220 32 657
Fragments arranged by size:
657 220 32
Enzymes that do cut:
AhdI AlwNI BanII BmgBI BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrFI BstYI Bsu36I BtgI BtsI DrdI EarI
EcoICRI Eco57MI FalI FseI Hin4I MmeI MspA1I NaeI
NcoI NdeI NgoMIV PasI PfoI PstI SacI SapI
SfcI SmlI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BsaAI BsaBI
BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII EaeI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NarI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI