Restriction map for
pFN21AE1790
(Linear) (Six-base) MAPSORT of: KIEE1790.seq Check: 2702 from: 1 to: 891
KIEE1790.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 703 891
Size: 703 188
AfeI AGC'GCT
Cuts at: 0 784 891
Size: 784 107
AflIII A'CryG_T
Cuts at: 0 365 470 891
Size: 365 105 421
Fragments arranged by size:
421 365 105
AleI CACnn'nnGTG
Cuts at: 0 739 891
Size: 739 152
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 457 489 891
Size: 457 32 402
Fragments arranged by size:
457 402 32
AlwNI CAG_nnn'CTG
Cuts at: 0 619 891
Size: 619 272
ApaLI G'TGCA_C
Cuts at: 0 361 891
Size: 361 530
AvaI C'yCGr_G
Cuts at: 0 163 180 301 891
Size: 163 17 121 590
Fragments arranged by size:
590 163 121 17
BanI G'GyrC_C
Cuts at: 0 96 501 792 891
Size: 96 405 291 99
Fragments arranged by size:
405 291 99 96
BanII G_rGCy'C
Cuts at: 0 731 891
Size: 731 160
BbeI G_GCGC'C
Cuts at: 0 100 891
Size: 100 791
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 390 424 891
Size: 390 34 467
Fragments arranged by size:
467 390 34
BglI GCCn_nnn'nGGC
Cuts at: 0 877 891
Size: 877 14
Bme1580I G_kGCm'C
Cuts at: 0 164 365 506 677 797 891
Size: 164 201 141 171 120 94
Fragments arranged by size:
201 171 164 141 120 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 372 891
Size: 372 519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 556 724 799 891
Size: 468 88 168 75 92
Fragments arranged by size:
468 168 92 88 75
Bpu10I CC'TnA_GC
Cuts at: 0 481 891
Size: 481 410
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 850 891
Size: 208 642 41
Fragments arranged by size:
642 208 41
BsaAI yAC'GTr
Cuts at: 0 243 636 666 741 891
Size: 243 393 30 75 150
Fragments arranged by size:
393 243 150 75 30
BsaHI Gr'CG_yC
Cuts at: 0 97 891
Size: 97 794
BseYI C'CCAG_C
Cuts at: 0 45 108 891
Size: 45 63 783
Fragments arranged by size:
783 63 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 570 891
Size: 570 321
BsiEI CG_ry'CG
Cuts at: 0 56 327 414 891
Size: 56 271 87 477
Fragments arranged by size:
477 271 87 56
BsiHKAI G_wGCw'C
Cuts at: 0 365 731 891
Size: 365 366 160
Fragments arranged by size:
366 365 160
BsiWI C'GTAC_G
Cuts at: 0 239 891
Size: 239 652
BsmI GAATG_Cn'
Cuts at: 0 525 891
Size: 525 366
BsmBI CGTCTCn'nnnn_
Cuts at: 0 313 891
Size: 313 578
Bsp1286I G_dGCh'C
Cuts at: 0 164 365 506 677 731 797 891
Size: 164 201 141 171 54 66 94
Fragments arranged by size:
201 171 164 141 94 66 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 703 891
Size: 703 188
BsrBI CCG'CTC
Cuts at: 0 813 891
Size: 813 78
BsrFI r'CCGG_y
Cuts at: 0 16 113 326 891
Size: 16 97 213 565
Fragments arranged by size:
565 213 97 16
BssHII G'CGCG_C
Cuts at: 0 272 891
Size: 272 619
BstEII G'GTnAC_C
Cuts at: 0 418 891
Size: 418 473
BstXI CCAn_nnnn'nTGG
Cuts at: 0 777 891
Size: 777 114
BtgI C'CryG_G
Cuts at: 0 131 174 267 312 396 414 461 891
Size: 131 43 93 45 84 18 47 430
Fragments arranged by size:
430 131 93 84 47 45 43 18
BtsI GCAGTG_nn'
Cuts at: 0 408 677 891
Size: 408 269 214
Fragments arranged by size:
408 269 214
DrdI GACnn_nn'nnGTC
Cuts at: 0 305 891
Size: 305 586
EaeI y'GGCC_r
Cuts at: 0 191 425 891
Size: 191 234 466
Fragments arranged by size:
466 234 191
EarI CTCTTCn'nnn_
Cuts at: 0 604 891
Size: 604 287
EcoICRI GAG'CTC
Cuts at: 0 729 891
Size: 729 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 556 724 799 891
Size: 468 88 168 75 92
Fragments arranged by size:
468 168 92 88 75
EcoO109I rG'GnC_Cy
Cuts at: 0 169 891
Size: 169 722
FseI GG_CCGG'CC
Cuts at: 0 20 891
Size: 20 871
HaeII r_GCGC'y
Cuts at: 0 10 100 786 891
Size: 10 90 686 105
Fragments arranged by size:
686 105 90 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 613 645 891
Size: 613 32 246
Fragments arranged by size:
613 246 32
KasI G'GCGC_C
Cuts at: 0 96 891
Size: 96 795
MluI A'CGCG_T
Cuts at: 0 365 891
Size: 365 526
MscI TGG'CCA
Cuts at: 0 193 891
Size: 193 698
MslI CAynn'nnrTG
Cuts at: 0 739 891
Size: 739 152
MspA1I CmG'CkG
Cuts at: 0 176 269 294 314 398 416 446 891
Size: 176 93 25 20 84 18 30 445
Fragments arranged by size:
445 176 93 84 30 25 20 18
NaeI GCC'GGC
Cuts at: 0 18 115 891
Size: 18 97 776
Fragments arranged by size:
776 97 18
NarI GG'CG_CC
Cuts at: 0 97 891
Size: 97 794
NcoI C'CATG_G
Cuts at: 0 461 891
Size: 461 430
NgoMIV G'CCGG_C
Cuts at: 0 16 113 891
Size: 16 97 778
Fragments arranged by size:
778 97 16
PfoI T'CCnGG_A
Cuts at: 0 701 891
Size: 701 190
PmlI CAC'GTG
Cuts at: 0 666 891
Size: 666 225
PpuMI rG'GwC_Cy
Cuts at: 0 169 891
Size: 169 722
PshAI GACnn'nnGTC
Cuts at: 0 416 891
Size: 416 475
PvuII CAG'CTG
Cuts at: 0 294 891
Size: 294 597
SacI G_AGCT'C
Cuts at: 0 731 891
Size: 731 160
SacII CC_GC'GG
Cuts at: 0 177 270 315 399 417 891
Size: 177 93 45 84 18 474
Fragments arranged by size:
474 177 93 84 45 18
SanDI GG'GwC_CC
Cuts at: 0 169 891
Size: 169 722
SexAI A'CCwGG_T
Cuts at: 0 644 891
Size: 644 247
SfoI GGC'GCC
Cuts at: 0 98 891
Size: 98 793
SmaI CCC'GGG
Cuts at: 0 165 182 891
Size: 165 17 709
Fragments arranged by size:
709 165 17
SmlI C'TyrA_G
Cuts at: 0 223 865 891
Size: 223 642 26
Fragments arranged by size:
642 223 26
SnaBI TAC'GTA
Cuts at: 0 636 891
Size: 636 255
SrfI GCCC'GGGC
Cuts at: 0 182 891
Size: 182 709
StyI C'CwwG_G
Cuts at: 0 461 891
Size: 461 430
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 270 891
Size: 37 233 621
Fragments arranged by size:
621 233 37
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 756 891
Size: 756 135
XmaI C'CCGG_G
Cuts at: 0 163 180 891
Size: 163 17 711
Fragments arranged by size:
711 163 17
Enzymes that do cut:
AarI AfeI AflIII AleI AloI AlwNI ApaLI AvaI
BanI BanII BbeI BcgI BglI Bme1580I BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BstEII BstXI BtgI BtsI DrdI EaeI EarI EcoICRI
Eco57MI EcoO109I FseI HaeII Hin4I KasI MluI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PfoI PmlI
PpuMI PshAI PvuII SacI SacII SanDI SexAI SfoI
SmaI SmlI SnaBI SrfI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AlfI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BsaI BsaBI BsaWI BsaXI BseRI
BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI
EciI EcoNI EcoRI EcoRV FalI FspI FspAI HincII
HindIII HpaI KpnI MfeI MmeI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PpiI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SpeI SphI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmnI ZraI