Restriction map for
pFN21AE1788
(Linear) (Six-base) MAPSORT of: KIEE1788.seq Check: 9222 from: 1 to: 885
KIEE1788.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 810 885
Size: 274 536 75
Fragments arranged by size:
536 274 75
AhdI GACnn_n'nnGTC
Cuts at: 0 709 885
Size: 709 176
AlwNI CAG_nnn'CTG
Cuts at: 0 252 885
Size: 252 633
ApaI G_GGCC'C
Cuts at: 0 475 885
Size: 475 410
ApaLI G'TGCA_C
Cuts at: 0 566 885
Size: 566 319
ApoI r'AATT_y
Cuts at: 0 217 885
Size: 217 668
BamHI G'GATC_C
Cuts at: 0 667 885
Size: 667 218
BanI G'GyrC_C
Cuts at: 0 581 885
Size: 581 304
BanII G_rGCy'C
Cuts at: 0 364 475 885
Size: 364 111 410
Fragments arranged by size:
410 364 111
BbeI G_GCGC'C
Cuts at: 0 585 885
Size: 585 300
BbsI GAAGACnn'nnnn_
Cuts at: 0 635 816 885
Size: 635 181 69
Fragments arranged by size:
635 181 69
BglI GCCn_nnn'nGGC
Cuts at: 0 95 104 885
Size: 95 9 781
Fragments arranged by size:
781 95 9
Bme1580I G_kGCm'C
Cuts at: 0 475 535 570 885
Size: 475 60 35 315
Fragments arranged by size:
475 315 60 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 436 885
Size: 436 449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 885
Size: 502 383
Bpu10I CC'TnA_GC
Cuts at: 0 625 885
Size: 625 260
BsaHI Gr'CG_yC
Cuts at: 0 582 885
Size: 582 303
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 20 83 885
Size: 20 63 802
Fragments arranged by size:
802 63 20
BseYI C'CCAG_C
Cuts at: 0 80 505 885
Size: 80 425 380
Fragments arranged by size:
425 380 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 885
Size: 141 744
BsiHKAI G_wGCw'C
Cuts at: 0 570 885
Size: 570 315
BsmI GAATG_Cn'
Cuts at: 0 448 885
Size: 448 437
Bsp1286I G_dGCh'C
Cuts at: 0 364 475 535 570 885
Size: 364 111 60 35 315
Fragments arranged by size:
364 315 111 60 35
BspHI T'CATG_A
Cuts at: 0 836 885
Size: 836 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 559 885
Size: 559 326
BsrBI CCG'CTC
Cuts at: 0 115 885
Size: 115 770
BsrDI GCAATG_nn'
Cuts at: 0 545 885
Size: 545 340
BstAPI GCAn_nnn'nTGC
Cuts at: 0 274 574 885
Size: 274 300 311
Fragments arranged by size:
311 300 274
BstEII G'GTnAC_C
Cuts at: 0 129 885
Size: 129 756
BstYI r'GATC_y
Cuts at: 0 667 885
Size: 667 218
BtgI C'CryG_G
Cuts at: 0 100 885
Size: 100 785
BtsI GCAGTG_nn'
Cuts at: 0 518 789 885
Size: 518 271 96
Fragments arranged by size:
518 271 96
DraI TTT'AAA
Cuts at: 0 215 885
Size: 215 670
EaeI y'GGCC_r
Cuts at: 0 848 885
Size: 848 37
EarI CTCTTCn'nnn_
Cuts at: 0 808 885
Size: 808 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 502 810 885
Size: 274 228 308 75
Fragments arranged by size:
308 274 228 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 885
Size: 256 629
EcoO109I rG'GnC_Cy
Cuts at: 0 305 472 885
Size: 305 167 413
Fragments arranged by size:
413 305 167
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 216 885
Size: 216 669
HaeII r_GCGC'y
Cuts at: 0 585 885
Size: 585 300
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 695 727 885
Size: 695 32 158
Fragments arranged by size:
695 158 32
HincII GTy'rAC
Cuts at: 0 239 501 885
Size: 239 262 384
Fragments arranged by size:
384 262 239
KasI G'GCGC_C
Cuts at: 0 581 885
Size: 581 304
MscI TGG'CCA
Cuts at: 0 850 885
Size: 850 35
MslI CAynn'nnrTG
Cuts at: 0 404 538 885
Size: 404 134 347
Fragments arranged by size:
404 347 134
NarI GG'CG_CC
Cuts at: 0 582 885
Size: 582 303
NdeI CA'TA_TG
Cuts at: 0 317 885
Size: 317 568
PasI CC'CwG_GG
Cuts at: 0 648 885
Size: 648 237
PspOMI G'GGCC_C
Cuts at: 0 471 885
Size: 471 414
PstI C_TGCA'G
Cuts at: 0 303 885
Size: 303 582
SfcI C'TryA_G
Cuts at: 0 299 885
Size: 299 586
SfoI GGC'GCC
Cuts at: 0 583 885
Size: 583 302
StyI C'CwwG_G
Cuts at: 0 356 885
Size: 356 529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 525 885
Size: 525 360
XmnI GAAnn'nnTTC
Cuts at: 0 658 885
Size: 658 227
Enzymes that do cut:
AcuI AhdI AlwNI ApaI ApaLI ApoI BamHI BanI
BanII BbeI BbsI BglI Bme1580I BmrI BpmI Bpu10I
BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BstAPI BstEII BstYI BtgI BtsI
DraI EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII KasI MscI MslI NarI NdeI PasI
PspOMI PstI SfcI SfoI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AscI AseI AsiSI AvaI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrFI BsrGI
BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI MspA1I NaeI NcoI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI ZraI