(Linear) (Six-base) MAPSORT of: KIEE1787.seq  Check: 622  from: 1  to: 882



KIEE1787.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:47  ..

AflIII A'CryG_T

Cuts at:      0     519     555     882 
   Size:        519      36     327

  Fragments arranged by size: 

                 519     327      36
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     688     720     882 
   Size:        688      32     162

  Fragments arranged by size: 

                 688     162      32
 
ApoI r'AATT_y

Cuts at:      0       3     294     478     820     882 
   Size:          3     291     184     342      62

  Fragments arranged by size: 

                 342     291     184      62       3
 
BamHI G'GATC_C

Cuts at:      0     454     882 
   Size:        454     428
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     319     584     882 
   Size:        319     265     298

  Fragments arranged by size: 

                 319     298     265
 
BsaAI yAC'GTr

Cuts at:      0     556     882 
   Size:        556     326
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     661     882 
   Size:        661     221
 
BsmI GAATG_Cn'

Cuts at:      0     250     393     882 
   Size:        250     143     489

  Fragments arranged by size: 

                 489     250     143
 
BspHI T'CATG_A

Cuts at:      0     263     761     882 
   Size:        263     498     121

  Fragments arranged by size: 

                 498     263     121
 
BsrDI GCAATG_nn'

Cuts at:      0     122     882 
   Size:        122     760
 
BstBI TT'CG_AA

Cuts at:      0     870     882 
   Size:        870      12
 
BstYI r'GATC_y

Cuts at:      0     454     882 
   Size:        454     428
 
BtgI C'CryG_G

Cuts at:      0      42     882 
   Size:         42     840
 
BtsI GCAGTG_nn'

Cuts at:      0     721     882 
   Size:        721     161
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     380     415     882 
   Size:        380      35     467

  Fragments arranged by size: 

                 467     380      35
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     586     882 
   Size:        586     296
 
EcoRI G'AATT_C

Cuts at:      0       3     294     882 
   Size:          3     291     588

  Fragments arranged by size: 

                 588     291       3
 
EcoRV GAT'ATC

Cuts at:      0     594     882 
   Size:        594     288
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     282     314     360     392     654     686     882 
   Size:        282      32      46      32     262      32     196

  Fragments arranged by size: 

                 282     262     196      46      32      32      32
 
NcoI C'CATG_G

Cuts at:      0      42     882 
   Size:         42     840
 
NspI r_CATG'y

Cuts at:      0     523     882 
   Size:        523     359
 
PciI A'CATG_T

Cuts at:      0     519     882 
   Size:        519     363
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     451     882 
   Size:        451     431
 
PfoI T'CCnGG_A

Cuts at:      0     239     450     626     882 
   Size:        239     211     176     256

  Fragments arranged by size: 

                 256     239     211     176
 
PmlI CAC'GTG

Cuts at:      0     556     882 
   Size:        556     326
 
SspI AAT'ATT

Cuts at:      0      76     882 
   Size:         76     806
 
StyI C'CwwG_G

Cuts at:      0      42     882 
   Size:         42     840
 
TatI w'GTAC_w

Cuts at:      0     558     882 
   Size:        558     324
 
XmnI GAAnn'nnTTC

Cuts at:      0     335     882 
   Size:        335     547
 

 Enzymes that do cut:

   AflIII     AloI     ApoI    BamHI     BbsI    BsaAI    BseRI     BsmI
    BspHI    BsrDI    BstBI    BstYI     BtgI     BtsI    CspCI    EcoNI
    EcoRI    EcoRV    Hin4I     NcoI     NspI     PciI    PflMI     PfoI
     PmlI     SspI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI