Restriction map for
pFN21AE1784
(Linear) (Six-base) MAPSORT of: KIEE1784.seq Check: 333 from: 1 to: 876
KIEE1784.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AccI GT'mk_AC
Cuts at: 0 6 876
Size: 6 870
AlwNI CAG_nnn'CTG
Cuts at: 0 75 327 876
Size: 75 252 549
Fragments arranged by size:
549 252 75
ApaI G_GGCC'C
Cuts at: 0 240 418 876
Size: 240 178 458
Fragments arranged by size:
458 240 178
AvaI C'yCGr_G
Cuts at: 0 560 650 876
Size: 560 90 226
Fragments arranged by size:
560 226 90
BanI G'GyrC_C
Cuts at: 0 151 607 732 876
Size: 151 456 125 144
Fragments arranged by size:
456 151 144 125
BanII G_rGCy'C
Cuts at: 0 240 290 418 697 760 876
Size: 240 50 128 279 63 116
Fragments arranged by size:
279 240 128 116 63 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 696 765 876
Size: 696 69 111
Fragments arranged by size:
696 111 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 198 232 876
Size: 198 34 644
Fragments arranged by size:
644 198 34
BclI T'GATC_A
Cuts at: 0 470 876
Size: 470 406
BglI GCCn_nnn'nGGC
Cuts at: 0 422 876
Size: 422 454
BlpI GC'TnA_GC
Cuts at: 0 142 876
Size: 142 734
Bme1580I G_kGCm'C
Cuts at: 0 154 240 418 737 876
Size: 154 86 178 319 139
Fragments arranged by size:
319 178 154 139 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 826 876
Size: 589 237 50
Fragments arranged by size:
589 237 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 234 876
Size: 234 642
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 7 37 876
Size: 7 30 839
Fragments arranged by size:
839 30 7
BseYI C'CCAG_C
Cuts at: 0 327 689 876
Size: 327 362 187
Fragments arranged by size:
362 327 187
BsiEI CG_ry'CG
Cuts at: 0 713 876
Size: 713 163
BsiHKAI G_wGCw'C
Cuts at: 0 143 876
Size: 143 733
Bsp1286I G_dGCh'C
Cuts at: 0 143 154 240 290 418 697 737 760
Size: 143 11 86 50 128 279 40 23
Cuts at: 760 876
Size: 116
Fragments arranged by size:
279 143 128 116 86 50 40 23
11
BsrDI GCAATG_nn'
Cuts at: 0 761 876
Size: 761 115
BsrFI r'CCGG_y
Cuts at: 0 56 430 712 876
Size: 56 374 282 164
Fragments arranged by size:
374 282 164 56
BssSI C'ACGA_G
Cuts at: 0 281 876
Size: 281 595
BstXI CCAn_nnnn'nTGG
Cuts at: 0 180 232 876
Size: 180 52 644
Fragments arranged by size:
644 180 52
BtgI C'CryG_G
Cuts at: 0 125 156 581 614 876
Size: 125 31 425 33 262
Fragments arranged by size:
425 262 125 33 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 201 876
Size: 201 675
EaeI y'GGCC_r
Cuts at: 0 77 222 527 714 876
Size: 77 145 305 187 162
Fragments arranged by size:
305 187 162 145 77
EarI CTCTTCn'nnn_
Cuts at: 0 827 876
Size: 827 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 826 876
Size: 589 237 50
Fragments arranged by size:
589 237 50
EcoO109I rG'GnC_Cy
Cuts at: 0 92 236 415 876
Size: 92 144 179 461
Fragments arranged by size:
461 179 144 92
HaeII r_GCGC'y
Cuts at: 0 76 876
Size: 76 800
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 876
Size: 7 32 837
Fragments arranged by size:
837 32 7
HincII GTy'rAC
Cuts at: 0 7 537 642 876
Size: 7 530 105 234
Fragments arranged by size:
530 234 105 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 848 876
Size: 90 758 28
Fragments arranged by size:
758 90 28
MscI TGG'CCA
Cuts at: 0 224 529 876
Size: 224 305 347
Fragments arranged by size:
347 305 224
MslI CAynn'nnrTG
Cuts at: 0 103 613 876
Size: 103 510 263
Fragments arranged by size:
510 263 103
MspA1I CmG'CkG
Cuts at: 0 29 786 876
Size: 29 757 90
Fragments arranged by size:
757 90 29
NaeI GCC'GGC
Cuts at: 0 432 876
Size: 432 444
NcoI C'CATG_G
Cuts at: 0 614 876
Size: 614 262
NgoMIV G'CCGG_C
Cuts at: 0 430 876
Size: 430 446
PpuMI rG'GwC_Cy
Cuts at: 0 92 876
Size: 92 784
PspOMI G'GGCC_C
Cuts at: 0 236 414 876
Size: 236 178 462
Fragments arranged by size:
462 236 178
PstI C_TGCA'G
Cuts at: 0 702 876
Size: 702 174
PvuII CAG'CTG
Cuts at: 0 786 876
Size: 786 90
SalI G'TCGA_C
Cuts at: 0 5 876
Size: 5 871
SanDI GG'GwC_CC
Cuts at: 0 92 876
Size: 92 784
SfcI C'TryA_G
Cuts at: 0 698 876
Size: 698 178
SmaI CCC'GGG
Cuts at: 0 652 876
Size: 652 224
StuI AGG'CCT
Cuts at: 0 566 574 876
Size: 566 8 302
Fragments arranged by size:
566 302 8
StyI C'CwwG_G
Cuts at: 0 614 876
Size: 614 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 169 331 720 876
Size: 169 162 389 156
Fragments arranged by size:
389 169 162 156
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 273 312 876
Size: 273 39 564
Fragments arranged by size:
564 273 39
XmaI C'CCGG_G
Cuts at: 0 650 876
Size: 650 226
Enzymes that do cut:
AccI AlwNI ApaI AvaI BanI BanII BbsI BcgI
BclI BglI BlpI Bme1580I BpmI BsaI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BsrDI BsrFI BssSI BstXI BtgI
BtgZI EaeI EarI Eco57MI EcoO109I HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PpuMI
PspOMI PstI PvuII SalI SanDI SfcI SmaI StuI
StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI
BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI