Restriction map for
pFN21AE1777
(Linear) (Six-base) MAPSORT of: KIEE1777.seq Check: 9767 from: 1 to: 855
KIEE1777.orf
With 182 enzymes: SgfI *
March 22, 2012 15:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 554 638 855
Size: 157 397 84 217
Fragments arranged by size:
397 217 157 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 389 855
Size: 389 466
AlwNI CAG_nnn'CTG
Cuts at: 0 661 855
Size: 661 194
BanI G'GyrC_C
Cuts at: 0 121 193 855
Size: 121 72 662
Fragments arranged by size:
662 121 72
BbeI G_GCGC'C
Cuts at: 0 197 855
Size: 197 658
BbsI GAAGACnn'nnnn_
Cuts at: 0 560 855
Size: 560 295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 111 145 855
Size: 111 34 710
Fragments arranged by size:
710 111 34
BclI T'GATC_A
Cuts at: 0 393 501 855
Size: 393 108 354
Fragments arranged by size:
393 354 108
BfrBI ATG'CAT
Cuts at: 0 366 370 855
Size: 366 4 485
Fragments arranged by size:
485 366 4
BlpI GC'TnA_GC
Cuts at: 0 168 855
Size: 168 687
Bme1580I G_kGCm'C
Cuts at: 0 124 855
Size: 124 731
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 114 855
Size: 114 741
BsaI GGTCTCn'nnnn_
Cuts at: 0 102 855
Size: 102 753
BsaAI yAC'GTr
Cuts at: 0 466 813 855
Size: 466 347 42
Fragments arranged by size:
466 347 42
BsaHI Gr'CG_yC
Cuts at: 0 194 855
Size: 194 661
BseYI C'CCAG_C
Cuts at: 0 117 855
Size: 117 738
BsiHKAI G_wGCw'C
Cuts at: 0 357 855
Size: 357 498
Bsp1286I G_dGCh'C
Cuts at: 0 124 357 855
Size: 124 233 498
Fragments arranged by size:
498 233 124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 161 855
Size: 161 694
BsrBI CCG'CTC
Cuts at: 0 71 284 855
Size: 71 213 571
Fragments arranged by size:
571 213 71
BssHII G'CGCG_C
Cuts at: 0 10 855
Size: 10 845
BstAPI GCAn_nnn'nTGC
Cuts at: 0 460 855
Size: 460 395
BstXI CCAn_nnnn'nTGG
Cuts at: 0 645 855
Size: 645 210
BstYI r'GATC_y
Cuts at: 0 430 855
Size: 430 425
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 187 855
Size: 187 668
BtsI GCAGTG_nn'
Cuts at: 0 21 844 855
Size: 21 823 11
Fragments arranged by size:
823 21 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 749 855
Size: 749 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 554 638 855
Size: 157 397 84 217
Fragments arranged by size:
397 217 157 84
HaeII r_GCGC'y
Cuts at: 0 197 679 855
Size: 197 482 176
Fragments arranged by size:
482 197 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 855
Size: 415 32 408
Fragments arranged by size:
415 408 32
KasI G'GCGC_C
Cuts at: 0 193 855
Size: 193 662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 580 728 855
Size: 453 127 148 127
Fragments arranged by size:
453 148 127 127
MslI CAynn'nnrTG
Cuts at: 0 365 371 643 784 855
Size: 365 6 272 141 71
Fragments arranged by size:
365 272 141 71 6
MspA1I CmG'CkG
Cuts at: 0 26 47 59 855
Size: 26 21 12 796
Fragments arranged by size:
796 26 21 12
NarI GG'CG_CC
Cuts at: 0 194 855
Size: 194 661
NdeI CA'TA_TG
Cuts at: 0 372 855
Size: 372 483
NsiI A_TGCA'T
Cuts at: 0 368 372 855
Size: 368 4 483
Fragments arranged by size:
483 368 4
NspI r_CATG'y
Cuts at: 0 366 370 458 855
Size: 366 4 88 397
Fragments arranged by size:
397 366 88 4
PflMI CCAn_nnn'nTGG
Cuts at: 0 747 855
Size: 747 108
PstI C_TGCA'G
Cuts at: 0 657 732 855
Size: 657 75 123
Fragments arranged by size:
657 123 75
SbfI CC_TGCA'GG
Cuts at: 0 732 855
Size: 732 123
SfcI C'TryA_G
Cuts at: 0 54 275 653 728 855
Size: 54 221 378 75 127
Fragments arranged by size:
378 221 127 75 54
SfoI GGC'GCC
Cuts at: 0 195 855
Size: 195 660
SphI G_CATG'C
Cuts at: 0 370 855
Size: 370 485
TatI w'GTAC_w
Cuts at: 0 478 579 855
Size: 478 101 276
Fragments arranged by size:
478 276 101
Enzymes that do cut:
AcuI AlfI AlwNI BanI BbeI BbsI BcgI BclI
BfrBI BlpI Bme1580I BplI BsaI BsaAI BsaHI BseYI
BsiHKAI Bsp1286I BspMI BsrBI BssHII BstAPI BstXI BstYI
BtgZI BtsI EciI Eco57MI HaeII Hin4I KasI MmeI
MslI MspA1I NarI NdeI NsiI NspI PflMI PstI
SbfI SfcI SfoI SphI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbvCI
BciVI BglI BglII BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssSI
BstBI BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI NaeI NcoI NgoMIV
NheI NotI NruI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI StuI StyI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI