Restriction map for
pFN21AE1775
(Linear) (Six-base) MAPSORT of: KIEE1775.seq Check: 5041 from: 1 to: 846
KIEE1775.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
Acc65I G'GTAC_C
Cuts at: 0 662 846
Size: 662 184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 846
Size: 769 77
AfeI AGC'GCT
Cuts at: 0 85 846
Size: 85 761
AgeI A'CCGG_T
Cuts at: 0 199 846
Size: 199 647
AlwNI CAG_nnn'CTG
Cuts at: 0 40 269 327 492 703 846
Size: 40 229 58 165 211 143
Fragments arranged by size:
229 211 165 143 58 40
ApaI G_GGCC'C
Cuts at: 0 591 648 846
Size: 591 57 198
Fragments arranged by size:
591 198 57
ApaLI G'TGCA_C
Cuts at: 0 475 846
Size: 475 371
AvaI C'yCGr_G
Cuts at: 0 51 846
Size: 51 795
BanI G'GyrC_C
Cuts at: 0 626 662 693 846
Size: 626 36 31 153
Fragments arranged by size:
626 153 36 31
BanII G_rGCy'C
Cuts at: 0 508 565 591 648 831 846
Size: 508 57 26 57 183 15
Fragments arranged by size:
508 183 57 57 26 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 807 846
Size: 807 39
BbvCI CC'TCA_GC
Cuts at: 0 613 846
Size: 613 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 207 241 846
Size: 207 34 605
Fragments arranged by size:
605 207 34
BglI GCCn_nnn'nGGC
Cuts at: 0 624 846
Size: 624 222
BglII A'GATC_T
Cuts at: 0 567 846
Size: 567 279
Bme1580I G_kGCm'C
Cuts at: 0 479 591 629 648 846
Size: 479 112 38 19 198
Fragments arranged by size:
479 198 112 38 19
BmgBI CAC'GTC
Cuts at: 0 474 846
Size: 474 372
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 527 846
Size: 527 319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 387 670 846
Size: 328 59 283 176
Fragments arranged by size:
328 283 176 59
Bpu10I CC'TnA_GC
Cuts at: 0 613 846
Size: 613 233
BsaI GGTCTCn'nnnn_
Cuts at: 0 540 846
Size: 540 306
BsaHI Gr'CG_yC
Cuts at: 0 155 527 812 846
Size: 155 372 285 34
Fragments arranged by size:
372 285 155 34
BsaWI w'CCGG_w
Cuts at: 0 199 211 846
Size: 199 12 635
Fragments arranged by size:
635 199 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 643 646 673 676 846
Size: 643 3 27 3 170
Fragments arranged by size:
643 170 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 361 622 625 667 721 724 846
Size: 361 261 3 42 54 3 122
Fragments arranged by size:
361 261 122 54 42 3 3
BseYI C'CCAG_C
Cuts at: 0 327 485 499 507 670 846
Size: 327 158 14 8 163 176
Fragments arranged by size:
327 176 163 158 14 8
BsiHKAI G_wGCw'C
Cuts at: 0 479 846
Size: 479 367
BsmI GAATG_Cn'
Cuts at: 0 418 846
Size: 418 428
Bsp1286I G_dGCh'C
Cuts at: 0 479 508 565 591 629 648 831 846
Size: 479 29 57 26 38 19 183 15
Fragments arranged by size:
479 183 57 38 29 26 19 15
BspEI T'CCGG_A
Cuts at: 0 211 846
Size: 211 635
BsrBI CCG'CTC
Cuts at: 0 5 846
Size: 5 841
BsrFI r'CCGG_y
Cuts at: 0 199 846
Size: 199 647
BsrGI T'GTAC_A
Cuts at: 0 374 846
Size: 374 472
BssHII G'CGCG_C
Cuts at: 0 96 846
Size: 96 750
BstYI r'GATC_y
Cuts at: 0 567 846
Size: 567 279
BtgI C'CryG_G
Cuts at: 0 163 846
Size: 163 683
DrdI GACnn_nn'nnGTC
Cuts at: 0 532 846
Size: 532 314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 387 670 769 846
Size: 328 59 283 99 77
Fragments arranged by size:
328 283 99 77 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 846
Size: 397 449
EcoO109I rG'GnC_Cy
Cuts at: 0 135 241 356 459 588 644 645 723
Size: 135 106 115 103 129 56 1 78
Cuts at: 723 753 846
Size: 30 93
Fragments arranged by size:
135 129 115 106 103 93 78 56
30 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 831 846
Size: 831 15
HaeII r_GCGC'y
Cuts at: 0 87 336 435 846
Size: 87 249 99 411
Fragments arranged by size:
411 249 99 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 206 238 552 584 846
Size: 206 32 314 32 262
Fragments arranged by size:
314 262 206 32 32
HincII GTy'rAC
Cuts at: 0 315 846
Size: 315 531
KpnI G_GTAC'C
Cuts at: 0 666 846
Size: 666 180
MslI CAynn'nnrTG
Cuts at: 0 292 472 846
Size: 292 180 374
Fragments arranged by size:
374 292 180
MspA1I CmG'CkG
Cuts at: 0 258 438 585 846
Size: 258 180 147 261
Fragments arranged by size:
261 258 180 147
NspI r_CATG'y
Cuts at: 0 375 388 846
Size: 375 13 458
Fragments arranged by size:
458 375 13
PasI CC'CwG_GG
Cuts at: 0 492 846
Size: 492 354
PflMI CCAn_nnn'nTGG
Cuts at: 0 327 492 846
Size: 327 165 354
Fragments arranged by size:
354 327 165
PfoI T'CCnGG_A
Cuts at: 0 305 749 846
Size: 305 444 97
Fragments arranged by size:
444 305 97
PpuMI rG'GwC_Cy
Cuts at: 0 135 459 753 846
Size: 135 324 294 93
Fragments arranged by size:
324 294 135 93
PspOMI G'GGCC_C
Cuts at: 0 587 644 846
Size: 587 57 202
Fragments arranged by size:
587 202 57
PstI C_TGCA'G
Cuts at: 0 274 344 846
Size: 274 70 502
Fragments arranged by size:
502 274 70
PvuII CAG'CTG
Cuts at: 0 258 438 846
Size: 258 180 408
Fragments arranged by size:
408 258 180
SfcI C'TryA_G
Cuts at: 0 270 340 846
Size: 270 70 506
Fragments arranged by size:
506 270 70
SgrAI Cr'CCGG_yG
Cuts at: 0 199 846
Size: 199 647
SmaI CCC'GGG
Cuts at: 0 53 846
Size: 53 793
StuI AGG'CCT
Cuts at: 0 618 846
Size: 618 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 484 846
Size: 484 362
TatI w'GTAC_w
Cuts at: 0 374 846
Size: 374 472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 643 675 846
Size: 643 32 171
Fragments arranged by size:
643 171 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 687 846
Size: 687 159
XmaI C'CCGG_G
Cuts at: 0 51 846
Size: 51 795
Enzymes that do cut:
Acc65I AcuI AfeI AgeI AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BbvCI BcgI BglI BglII Bme1580I
BmgBI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrFI
BsrGI BssHII BstYI BtgI DrdI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KpnI MslI MspA1I NspI
PasI PflMI PfoI PpuMI PspOMI PstI PvuII SfcI
SgrAI SmaI StuI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BlpI BmrI BmtI
BpuEI BsaAI BsaBI BsgI BsiEI BsiWI BsmBI BspHI
BspMI BsrDI BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EaeI
EagI EarI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI Tth111I
XbaI XhoI XmnI ZraI