Restriction map for
pFN21AE1771
(Linear) (Six-base) MAPSORT of: KIEE1771.seq Check: 818 from: 1 to: 831
KIEE1771.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 205 238 831
Size: 34 171 33 593
Fragments arranged by size:
593 171 34 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 763 831
Size: 763 68
ApaLI G'TGCA_C
Cuts at: 0 410 831
Size: 410 421
ApoI r'AATT_y
Cuts at: 0 189 369 831
Size: 189 180 462
Fragments arranged by size:
462 189 180
AvaI C'yCGr_G
Cuts at: 0 52 831
Size: 52 779
BanI G'GyrC_C
Cuts at: 0 162 831
Size: 162 669
BanII G_rGCy'C
Cuts at: 0 554 831
Size: 554 277
BbvCI CC'TCA_GC
Cuts at: 0 139 831
Size: 139 692
Bme1580I G_kGCm'C
Cuts at: 0 414 831
Size: 414 417
Bpu10I CC'TnA_GC
Cuts at: 0 139 831
Size: 139 692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 381 831
Size: 352 29 450
Fragments arranged by size:
450 352 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 241 831
Size: 43 198 590
Fragments arranged by size:
590 198 43
BseYI C'CCAG_C
Cuts at: 0 539 831
Size: 539 292
BsiHKAI G_wGCw'C
Cuts at: 0 203 414 554 831
Size: 203 211 140 277
Fragments arranged by size:
277 211 203 140
Bsp1286I G_dGCh'C
Cuts at: 0 203 414 554 831
Size: 203 211 140 277
Fragments arranged by size:
277 211 203 140
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 615 831
Size: 615 216
BsrDI GCAATG_nn'
Cuts at: 0 662 831
Size: 662 169
BssSI C'ACGA_G
Cuts at: 0 305 831
Size: 305 526
BstEII G'GTnAC_C
Cuts at: 0 654 831
Size: 654 177
BstXI CCAn_nnnn'nTGG
Cuts at: 0 90 831
Size: 90 741
Bsu36I CC'TnA_GG
Cuts at: 0 490 518 831
Size: 490 28 313
Fragments arranged by size:
490 313 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 159 831
Size: 159 672
EaeI y'GGCC_r
Cuts at: 0 615 831
Size: 615 216
EarI CTCTTCn'nnn_
Cuts at: 0 380 831
Size: 380 451
EcoICRI GAG'CTC
Cuts at: 0 552 831
Size: 552 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 205 238 831
Size: 34 171 33 593
Fragments arranged by size:
593 171 34 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 831
Size: 493 32 306
Fragments arranged by size:
493 306 32
HincII GTy'rAC
Cuts at: 0 99 831
Size: 99 732
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 831
Size: 107 724
MscI TGG'CCA
Cuts at: 0 617 831
Size: 617 214
NspI r_CATG'y
Cuts at: 0 90 750 831
Size: 90 660 81
Fragments arranged by size:
660 90 81
PasI CC'CwG_GG
Cuts at: 0 650 831
Size: 650 181
PflMI CCAn_nnn'nTGG
Cuts at: 0 457 831
Size: 457 374
PfoI T'CCnGG_A
Cuts at: 0 378 831
Size: 378 453
SacI G_AGCT'C
Cuts at: 0 554 831
Size: 554 277
SmlI C'TyrA_G
Cuts at: 0 331 360 831
Size: 331 29 471
Fragments arranged by size:
471 331 29
StyI C'CwwG_G
Cuts at: 0 276 831
Size: 276 555
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 831
Size: 642 189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 119 151 831
Size: 119 32 680
Fragments arranged by size:
680 119 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 679 831
Size: 679 152
Enzymes that do cut:
AcuI AlfI ApaLI ApoI AvaI BanI BanII BbvCI
Bme1580I Bpu10I BpuEI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrDI BssSI BstEII BstXI Bsu36I BtgZI EaeI EarI
EcoICRI Eco57MI Hin4I HincII MmeI MscI NspI PasI
PflMI PfoI SacI SmlI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BstAPI BstBI BstYI BstZ17I BtgI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI