(Linear) (Six-base) MAPSORT of: KIEE1769.seq  Check: 7622  from: 1  to: 831



KIEE1769.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     683     831 
   Size:        683     148
 
ApaI G_GGCC'C

Cuts at:      0     302     831 
   Size:        302     529
 
ApoI r'AATT_y

Cuts at:      0      68     175     431     831 
   Size:         68     107     256     400

  Fragments arranged by size: 

                 400     256     107      68
 
BamHI G'GATC_C

Cuts at:      0     741     831 
   Size:        741      90
 
BanII G_rGCy'C

Cuts at:      0     302     831 
   Size:        302     529
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      44     831 
   Size:         44     787
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     271     759     831 
   Size:        271     488      72

  Fragments arranged by size: 

                 488     271      72
 
BclI T'GATC_A

Cuts at:      0       2     831 
   Size:          2     829
 
Bme1580I G_kGCm'C

Cuts at:      0     302     831 
   Size:        302     529
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     428     731     831 
   Size:        428     303     100

  Fragments arranged by size: 

                 428     303     100
 
Bpu10I CC'TnA_GC

Cuts at:      0     161     263     831 
   Size:        161     102     568

  Fragments arranged by size: 

                 568     161     102
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     484     831 
   Size:         29     455     347

  Fragments arranged by size: 

                 455     347      29
 
BsaHI Gr'CG_yC

Cuts at:      0     410     831 
   Size:        410     421
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     352     831 
   Size:        352     479
 
Bsp1286I G_dGCh'C

Cuts at:      0     302     831 
   Size:        302     529
 
BspHI T'CATG_A

Cuts at:      0     671     831 
   Size:        671     160
 
BsrDI GCAATG_nn'

Cuts at:      0     743     831 
   Size:        743      88
 
BsrFI r'CCGG_y

Cuts at:      0     103     831 
   Size:        103     728
 
BstYI r'GATC_y

Cuts at:      0     420     741     831 
   Size:        420     321      90

  Fragments arranged by size: 

                 420     321      90
 
BtgI C'CryG_G

Cuts at:      0     606     831 
   Size:        606     225
 
DraI TTT'AAA

Cuts at:      0     438     831 
   Size:        438     393
 
EaeI y'GGCC_r

Cuts at:      0     186     831 
   Size:        186     645
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     428     683     731     831 
   Size:        428     255      48     100

  Fragments arranged by size: 

                 428     255     100      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     298     831 
   Size:        298     533
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     713     800     831 
   Size:        713      87      31

  Fragments arranged by size: 

                 713      87      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     224     256     733     765     831 
   Size:        224      32     477      32      66

  Fragments arranged by size: 

                 477     224      66      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     255     831 
   Size:        255     576
 
MslI CAynn'nnrTG

Cuts at:      0     242     831 
   Size:        242     589
 
MspA1I CmG'CkG

Cuts at:      0     709     831 
   Size:        709     122
 
NcoI C'CATG_G

Cuts at:      0     606     831 
   Size:        606     225
 
NdeI CA'TA_TG

Cuts at:      0      85     831 
   Size:         85     746
 
PspOMI G'GGCC_C

Cuts at:      0     298     831 
   Size:        298     533
 
PvuII CAG'CTG

Cuts at:      0     709     831 
   Size:        709     122
 
ScaI AGT'ACT

Cuts at:      0     780     831 
   Size:        780      51
 
SmlI C'TyrA_G

Cuts at:      0      44     499     831 
   Size:         44     455     332

  Fragments arranged by size: 

                 455     332      44
 
StyI C'CwwG_G

Cuts at:      0     606     831 
   Size:        606     225
 
TatI w'GTAC_w

Cuts at:      0     658     778     831 
   Size:        658     120      53

  Fragments arranged by size: 

                 658     120      53
 

 Enzymes that do cut:

     AcuI     ApaI     ApoI    BamHI    BanII     BbsI    BciVI     BclI
 Bme1580I     BpmI   Bpu10I    BpuEI    BsaHI    BsmBI Bsp1286I    BspHI
    BsrDI    BsrFI    BstYI     BtgI     DraI     EaeI  Eco57MI EcoO109I
     FalI    Hin4I     MmeI     MslI   MspA1I     NcoI     NdeI   PspOMI
    PvuII     ScaI     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI
     BcgI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspMI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI