Restriction map for
pFN21AE1768
(Linear) (Six-base) MAPSORT of: KIEE1768.seq Check: 1458 from: 1 to: 831
KIEE1768.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 831
Size: 596 235
AgeI A'CCGG_T
Cuts at: 0 119 831
Size: 119 712
AlwNI CAG_nnn'CTG
Cuts at: 0 345 735 831
Size: 345 390 96
Fragments arranged by size:
390 345 96
ApaI G_GGCC'C
Cuts at: 0 544 688 831
Size: 544 144 143
Fragments arranged by size:
544 144 143
ApaLI G'TGCA_C
Cuts at: 0 294 831
Size: 294 537
AvaI C'yCGr_G
Cuts at: 0 628 831
Size: 628 203
BanI G'GyrC_C
Cuts at: 0 334 831
Size: 334 497
BanII G_rGCy'C
Cuts at: 0 15 86 544 688 831
Size: 15 71 458 144 143
Fragments arranged by size:
458 144 143 71 15
BglI GCCn_nnn'nGGC
Cuts at: 0 332 831
Size: 332 499
BlpI GC'TnA_GC
Cuts at: 0 67 831
Size: 67 764
Bme1580I G_kGCm'C
Cuts at: 0 298 337 378 395 544 688 831
Size: 298 39 41 17 149 144 143
Fragments arranged by size:
298 149 144 143 41 39 17
BmgBI CAC'GTC
Cuts at: 0 222 831
Size: 222 609
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 383 831
Size: 371 12 448
Fragments arranged by size:
448 371 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 526 831
Size: 526 305
BsaAI yAC'GTr
Cuts at: 0 254 831
Size: 254 577
BsaHI Gr'CG_yC
Cuts at: 0 806 831
Size: 806 25
BsaWI w'CCGG_w
Cuts at: 0 119 719 776 831
Size: 119 600 57 55
Fragments arranged by size:
600 119 57 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 796 826 831
Size: 796 30 5
Fragments arranged by size:
796 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 808 831
Size: 40 768 23
Fragments arranged by size:
768 40 23
BseYI C'CCAG_C
Cuts at: 0 21 109 338 448 513 672 681 831
Size: 21 88 229 110 65 159 9 150
Fragments arranged by size:
229 159 150 110 88 65 21 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 234 423 831
Size: 156 78 189 408
Fragments arranged by size:
408 189 156 78
BsiEI CG_ry'CG
Cuts at: 0 120 831
Size: 120 711
BsiHKAI G_wGCw'C
Cuts at: 0 73 298 831
Size: 73 225 533
Fragments arranged by size:
533 225 73
Bsp1286I G_dGCh'C
Cuts at: 0 15 73 86 298 337 378 395 544
Size: 15 58 13 212 39 41 17 149
Cuts at: 544 688 831
Size: 144 143
Fragments arranged by size:
212 149 144 143 58 41 39 17
15 13
BspEI T'CCGG_A
Cuts at: 0 719 776 831
Size: 719 57 55
Fragments arranged by size:
719 57 55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 722 831
Size: 722 109
BsrBI CCG'CTC
Cuts at: 0 173 282 831
Size: 173 109 549
Fragments arranged by size:
549 173 109
BsrFI r'CCGG_y
Cuts at: 0 119 468 831
Size: 119 349 363
Fragments arranged by size:
363 349 119
BssSI C'ACGA_G
Cuts at: 0 130 831
Size: 130 701
BstAPI GCAn_nnn'nTGC
Cuts at: 0 248 462 831
Size: 248 214 369
Fragments arranged by size:
369 248 214
BstXI CCAn_nnnn'nTGG
Cuts at: 0 702 831
Size: 702 129
BtgI C'CryG_G
Cuts at: 0 27 617 831
Size: 27 590 214
Fragments arranged by size:
590 214 27
DraIII CAC_nnn'GTG
Cuts at: 0 188 257 831
Size: 188 69 574
Fragments arranged by size:
574 188 69
EaeI y'GGCC_r
Cuts at: 0 299 470 548 692 821 831
Size: 299 171 78 144 129 10
Fragments arranged by size:
299 171 144 129 78 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 383 596 831
Size: 371 12 213 235
Fragments arranged by size:
371 235 213 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 764 831
Size: 764 67
EcoO109I rG'GnC_Cy
Cuts at: 0 507 540 541 601 624 685 831
Size: 507 33 1 60 23 61 146
Fragments arranged by size:
507 146 61 60 33 23 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 796 828 831
Size: 796 32 3
Fragments arranged by size:
796 32 3
HincII GTy'rAC
Cuts at: 0 369 831
Size: 369 462
MscI TGG'CCA
Cuts at: 0 694 823 831
Size: 694 129 8
Fragments arranged by size:
694 129 8
MspA1I CmG'CkG
Cuts at: 0 29 164 291 323 831
Size: 29 135 127 32 508
Fragments arranged by size:
508 135 127 32 29
NaeI GCC'GGC
Cuts at: 0 470 831
Size: 470 361
NgoMIV G'CCGG_C
Cuts at: 0 468 831
Size: 468 363
PasI CC'CwG_GG
Cuts at: 0 537 760 831
Size: 537 223 71
Fragments arranged by size:
537 223 71
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 831
Size: 345 486
PmlI CAC'GTG
Cuts at: 0 254 831
Size: 254 577
PpuMI rG'GwC_Cy
Cuts at: 0 507 601 624 831
Size: 507 94 23 207
Fragments arranged by size:
507 207 94 23
PspOMI G'GGCC_C
Cuts at: 0 540 684 831
Size: 540 144 147
Fragments arranged by size:
540 147 144
PstI C_TGCA'G
Cuts at: 0 169 467 831
Size: 169 298 364
Fragments arranged by size:
364 298 169
PvuII CAG'CTG
Cuts at: 0 291 831
Size: 291 540
SacII CC_GC'GG
Cuts at: 0 30 831
Size: 30 801
SanDI GG'GwC_CC
Cuts at: 0 507 601 831
Size: 507 94 230
Fragments arranged by size:
507 230 94
SfcI C'TryA_G
Cuts at: 0 165 463 831
Size: 165 298 368
Fragments arranged by size:
368 298 165
SmaI CCC'GGG
Cuts at: 0 630 831
Size: 630 201
StuI AGG'CCT
Cuts at: 0 418 831
Size: 418 413
StyI C'CwwG_G
Cuts at: 0 443 831
Size: 443 388
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 352 831
Size: 352 479
Tth111I GACn'n_nGTC
Cuts at: 0 223 313 831
Size: 223 90 518
Fragments arranged by size:
518 223 90
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 615 657 757 831
Size: 441 174 42 100 74
Fragments arranged by size:
441 174 100 74 42
XmaI C'CCGG_G
Cuts at: 0 628 831
Size: 628 203
Enzymes that do cut:
AcuI AgeI AlwNI ApaI ApaLI AvaI BanI BanII
BglI BlpI Bme1580I BmgBI BpmI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrFI BssSI BstAPI BstXI BtgI
DraIII EaeI Eco57MI EcoNI EcoO109I Hin4I HincII MscI
MspA1I NaeI NgoMIV PasI PflMI PmlI PpuMI PspOMI
PstI PvuII SacII SanDI SfcI SmaI StuI StyI
TaqII Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BmrI BmtI BplI Bpu10I BpuEI
BsaBI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI XbaI XhoI XmnI ZraI