Restriction map for
pFN21AE1766
(Linear) (Six-base) MAPSORT of: KIEE1766.seq Check: 9282 from: 1 to: 813
KIEE1766.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 55 813
Size: 55 758
AflIII A'CryG_T
Cuts at: 0 803 813
Size: 803 10
AleI CACnn'nnGTG
Cuts at: 0 350 813
Size: 350 463
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4 36 813
Size: 4 32 777
Fragments arranged by size:
777 32 4
AlwNI CAG_nnn'CTG
Cuts at: 0 706 813
Size: 706 107
ApaI G_GGCC'C
Cuts at: 0 568 813
Size: 568 245
BamHI G'GATC_C
Cuts at: 0 630 813
Size: 630 183
BanII G_rGCy'C
Cuts at: 0 568 723 813
Size: 568 155 90
Fragments arranged by size:
568 155 90
BbsI GAAGACnn'nnnn_
Cuts at: 0 593 813
Size: 593 220
BglII A'GATC_T
Cuts at: 0 640 813
Size: 640 173
Bme1580I G_kGCm'C
Cuts at: 0 568 713 813
Size: 568 145 100
Fragments arranged by size:
568 145 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 683 813
Size: 31 652 130
Fragments arranged by size:
652 130 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 398 813
Size: 398 415
BsaWI w'CCGG_w
Cuts at: 0 200 506 813
Size: 200 306 307
Fragments arranged by size:
307 306 200
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 376 406 813
Size: 4 30 342 30 407
Fragments arranged by size:
407 342 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 397 813
Size: 397 416
BseYI C'CCAG_C
Cuts at: 0 166 813
Size: 166 647
BsiHKAI G_wGCw'C
Cuts at: 0 660 723 813
Size: 660 63 90
Fragments arranged by size:
660 90 63
Bsp1286I G_dGCh'C
Cuts at: 0 568 660 713 723 813
Size: 568 92 53 10 90
Fragments arranged by size:
568 92 90 53 10
BspEI T'CCGG_A
Cuts at: 0 506 813
Size: 506 307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 55 813
Size: 55 758
BsrFI r'CCGG_y
Cuts at: 0 36 813
Size: 36 777
BstEII G'GTnAC_C
Cuts at: 0 121 813
Size: 121 692
BstXI CCAn_nnnn'nTGG
Cuts at: 0 170 252 495 813
Size: 170 82 243 318
Fragments arranged by size:
318 243 170 82
BstYI r'GATC_y
Cuts at: 0 630 640 813
Size: 630 10 173
Fragments arranged by size:
630 173 10
Bsu36I CC'TnA_GG
Cuts at: 0 675 813
Size: 675 138
BtgI C'CryG_G
Cuts at: 0 251 813
Size: 251 562
BtsI GCAGTG_nn'
Cuts at: 0 439 813
Size: 439 374
ClaI AT'CG_AT
Cuts at: 0 335 813
Size: 335 478
DrdI GACnn_nn'nnGTC
Cuts at: 0 64 193 813
Size: 64 129 620
Fragments arranged by size:
620 129 64
EaeI y'GGCC_r
Cuts at: 0 38 813
Size: 38 775
EcoICRI GAG'CTC
Cuts at: 0 721 813
Size: 721 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 683 813
Size: 31 652 130
Fragments arranged by size:
652 130 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 611 674 813
Size: 611 63 139
Fragments arranged by size:
611 139 63
EcoO109I rG'GnC_Cy
Cuts at: 0 133 365 813
Size: 133 232 448
Fragments arranged by size:
448 232 133
HaeII r_GCGC'y
Cuts at: 0 37 707 813
Size: 37 670 106
Fragments arranged by size:
670 106 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 376 408 615 647 658 690
Size: 133 32 211 32 207 32 11 32
Cuts at: 690 813
Size: 123
Fragments arranged by size:
211 207 133 123 32 32 32 32
11
MslI CAynn'nnrTG
Cuts at: 0 250 350 625 813
Size: 250 100 275 188
Fragments arranged by size:
275 250 188 100
MspA1I CmG'CkG
Cuts at: 0 166 813
Size: 166 647
NaeI GCC'GGC
Cuts at: 0 38 813
Size: 38 775
NgoMIV G'CCGG_C
Cuts at: 0 36 813
Size: 36 777
NspI r_CATG'y
Cuts at: 0 807 813
Size: 807 6
PasI CC'CwG_GG
Cuts at: 0 561 813
Size: 561 252
PciI A'CATG_T
Cuts at: 0 803 813
Size: 803 10
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 813
Size: 4 32 777
Fragments arranged by size:
777 32 4
PpuMI rG'GwC_Cy
Cuts at: 0 133 365 813
Size: 133 232 448
Fragments arranged by size:
448 232 133
PspOMI G'GGCC_C
Cuts at: 0 564 813
Size: 564 249
PstI C_TGCA'G
Cuts at: 0 52 813
Size: 52 761
PvuII CAG'CTG
Cuts at: 0 166 813
Size: 166 647
SacI G_AGCT'C
Cuts at: 0 723 813
Size: 723 90
SanDI GG'GwC_CC
Cuts at: 0 133 813
Size: 133 680
SbfI CC_TGCA'GG
Cuts at: 0 52 813
Size: 52 761
SfcI C'TryA_G
Cuts at: 0 48 813
Size: 48 765
StuI AGG'CCT
Cuts at: 0 680 813
Size: 680 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 103 158 340 380 539 813
Size: 103 55 182 40 159 274
Fragments arranged by size:
274 182 159 103 55 40
Enzymes that do cut:
AarI AflIII AleI AloI AlwNI ApaI BamHI BanII
BbsI BglII Bme1580I BpmI BsaI BsaWI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BspEI BspMI BsrFI BstEII BstXI
BstYI Bsu36I BtgI BtsI ClaI DrdI EaeI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I MslI MspA1I NaeI
NgoMIV NspI PasI PciI PpiI PpuMI PspOMI PstI
PvuII SacI SanDI SbfI SfcI StuI TaqII
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BsmI
BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I BtgZI CspCI DraI DraIII EagI EarI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StyI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI