Restriction map for
pFN21AE1764
(Linear) (Six-base) MAPSORT of: KIEE1764.seq Check: 961 from: 1 to: 810
KIEE1764.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AfeI AGC'GCT
Cuts at: 0 595 810
Size: 595 215
AgeI A'CCGG_T
Cuts at: 0 764 810
Size: 764 46
ApoI r'AATT_y
Cuts at: 0 175 810
Size: 175 635
AvaI C'yCGr_G
Cuts at: 0 72 810
Size: 72 738
BanI G'GyrC_C
Cuts at: 0 79 159 603 810
Size: 79 80 444 207
Fragments arranged by size:
444 207 80 79
BanII G_rGCy'C
Cuts at: 0 419 572 692 810
Size: 419 153 120 118
Fragments arranged by size:
419 153 120 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 810
Size: 131 679
BglI GCCn_nnn'nGGC
Cuts at: 0 437 810
Size: 437 373
BlpI GC'TnA_GC
Cuts at: 0 751 810
Size: 751 59
Bme1580I G_kGCm'C
Cuts at: 0 82 606 810
Size: 82 524 204
Fragments arranged by size:
524 204 82
BmgBI CAC'GTC
Cuts at: 0 699 810
Size: 699 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 318 616 810
Size: 52 266 298 194
Fragments arranged by size:
298 266 194 52
Bpu10I CC'TnA_GC
Cuts at: 0 686 810
Size: 686 124
BsaHI Gr'CG_yC
Cuts at: 0 55 128 810
Size: 55 73 682
Fragments arranged by size:
682 73 55
BsaWI w'CCGG_w
Cuts at: 0 764 810
Size: 764 46
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 614 644 731 761 810
Size: 614 30 87 30 49
Fragments arranged by size:
614 87 49 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 810
Size: 619 191
BsiHKAI G_wGCw'C
Cuts at: 0 520 752 810
Size: 520 232 58
Fragments arranged by size:
520 232 58
Bsp1286I G_dGCh'C
Cuts at: 0 82 419 520 572 606 692 752 810
Size: 82 337 101 52 34 86 60 58
Fragments arranged by size:
337 101 86 82 60 58 52 34
BsrBI CCG'CTC
Cuts at: 0 36 810
Size: 36 774
BsrFI r'CCGG_y
Cuts at: 0 764 810
Size: 764 46
BssHII G'CGCG_C
Cuts at: 0 21 810
Size: 21 789
BstYI r'GATC_y
Cuts at: 0 104 348 810
Size: 104 244 462
Fragments arranged by size:
462 244 104
BtsI GCAGTG_nn'
Cuts at: 0 21 810
Size: 21 789
EaeI y'GGCC_r
Cuts at: 0 45 232 810
Size: 45 187 578
Fragments arranged by size:
578 187 45
EarI CTCTTCn'nnn_
Cuts at: 0 142 584 810
Size: 142 442 226
Fragments arranged by size:
442 226 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 275 810
Size: 275 535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 318 616 810
Size: 52 266 298 194
Fragments arranged by size:
298 266 194 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 646 810
Size: 646 164
EcoO109I rG'GnC_Cy
Cuts at: 0 284 553 810
Size: 284 269 257
Fragments arranged by size:
284 269 257
EcoRI G'AATT_C
Cuts at: 0 175 810
Size: 175 635
HaeII r_GCGC'y
Cuts at: 0 597 810
Size: 597 213
HincII GTy'rAC
Cuts at: 0 447 810
Size: 447 363
HpaI GTT'AAC
Cuts at: 0 447 810
Size: 447 363
MscI TGG'CCA
Cuts at: 0 47 810
Size: 47 763
MslI CAynn'nnrTG
Cuts at: 0 460 810
Size: 460 350
PfoI T'CCnGG_A
Cuts at: 0 344 810
Size: 344 466
PpuMI rG'GwC_Cy
Cuts at: 0 284 553 810
Size: 284 269 257
Fragments arranged by size:
284 269 257
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 700 732 810
Size: 700 32 78
Fragments arranged by size:
700 78 32
SapI GCTCTTCn'nnn_
Cuts at: 0 584 810
Size: 584 226
StyI C'CwwG_G
Cuts at: 0 83 810
Size: 83 727
TatI w'GTAC_w
Cuts at: 0 371 810
Size: 371 439
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 379 394 411 426 614 646 651 683
Size: 379 15 17 15 188 32 5 32
Cuts at: 683 731 763 810
Size: 48 32 47
Fragments arranged by size:
379 188 48 47 32 32 32 17
15 15 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 810
Size: 108 702
Enzymes that do cut:
AfeI AgeI ApoI AvaI BanI BanII BbsI BglI
BlpI Bme1580I BmgBI BpmI Bpu10I BsaHI BsaWI BsaXI
BseRI BsiHKAI Bsp1286I BsrBI BsrFI BssHII BstYI BtsI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII
HincII HpaI MscI MslI PfoI PpuMI PsrI SapI
StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BseYI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI