Restriction map for
pFN21AE1763
(Linear) (Six-base) MAPSORT of: KIEE1763.seq Check: 7795 from: 1 to: 807
KIEE1763.orf
With 182 enzymes: SgfI *
July 15, 2010 18:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 107 807
Size: 107 700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 602 807
Size: 499 103 205
Fragments arranged by size:
499 205 103
AfeI AGC'GCT
Cuts at: 0 130 807
Size: 130 677
AflIII A'CryG_T
Cuts at: 0 404 807
Size: 404 403
AlwNI CAG_nnn'CTG
Cuts at: 0 741 807
Size: 741 66
ApaI G_GGCC'C
Cuts at: 0 309 807
Size: 309 498
BamHI G'GATC_C
Cuts at: 0 392 807
Size: 392 415
BanI G'GyrC_C
Cuts at: 0 66 240 360 807
Size: 66 174 120 447
Fragments arranged by size:
447 174 120 66
BanII G_rGCy'C
Cuts at: 0 309 807
Size: 309 498
BbeI G_GCGC'C
Cuts at: 0 70 244 807
Size: 70 174 563
Fragments arranged by size:
563 174 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 276 807
Size: 276 531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 198 232 807
Size: 198 34 575
Fragments arranged by size:
575 198 34
Bme1580I G_kGCm'C
Cuts at: 0 309 807
Size: 309 498
BmrI ACTGGGnnnn_n'
Cuts at: 0 312 807
Size: 312 495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 584 659 807
Size: 214 370 75 148
Fragments arranged by size:
370 214 148 75
Bpu10I CC'TnA_GC
Cuts at: 0 758 807
Size: 758 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 418 602 807
Size: 130 288 184 205
Fragments arranged by size:
288 205 184 130
BsaAI yAC'GTr
Cuts at: 0 407 807
Size: 407 400
BsaHI Gr'CG_yC
Cuts at: 0 67 241 281 491 807
Size: 67 174 40 210 316
Fragments arranged by size:
316 210 174 67 40
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 499 529 807
Size: 499 30 278
Fragments arranged by size:
499 278 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 162 436 807
Size: 162 274 371
Fragments arranged by size:
371 274 162
BseYI C'CCAG_C
Cuts at: 0 10 131 772 807
Size: 10 121 641 35
Fragments arranged by size:
641 121 35 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 807
Size: 253 554
Bsp1286I G_dGCh'C
Cuts at: 0 309 807
Size: 309 498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 807
Size: 107 700
BsrDI GCAATG_nn'
Cuts at: 0 279 807
Size: 279 528
BsrFI r'CCGG_y
Cuts at: 0 117 807
Size: 117 690
BstAPI GCAn_nnn'nTGC
Cuts at: 0 741 807
Size: 741 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 780 807
Size: 780 27
BstYI r'GATC_y
Cuts at: 0 392 807
Size: 392 415
BtgI C'CryG_G
Cuts at: 0 71 310 591 807
Size: 71 239 281 216
Fragments arranged by size:
281 239 216 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 201 807
Size: 201 606
BtsI GCAGTG_nn'
Cuts at: 0 742 758 807
Size: 742 16 49
Fragments arranged by size:
742 49 16
EaeI y'GGCC_r
Cuts at: 0 158 212 807
Size: 158 54 595
Fragments arranged by size:
595 158 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 499 584 602 659 807
Size: 214 285 85 18 57 148
Fragments arranged by size:
285 214 148 85 57 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 807
Size: 349 458
EcoO109I rG'GnC_Cy
Cuts at: 0 189 306 664 807
Size: 189 117 358 143
Fragments arranged by size:
358 189 143 117
FspI TGC'GCA
Cuts at: 0 103 807
Size: 103 704
HaeII r_GCGC'y
Cuts at: 0 8 70 132 244 807
Size: 8 62 62 112 563
Fragments arranged by size:
563 112 62 62 8
KasI G'GCGC_C
Cuts at: 0 66 240 807
Size: 66 174 567
Fragments arranged by size:
567 174 66
MscI TGG'CCA
Cuts at: 0 214 807
Size: 214 593
MslI CAynn'nnrTG
Cuts at: 0 386 559 807
Size: 386 173 248
Fragments arranged by size:
386 248 173
MspA1I CmG'CkG
Cuts at: 0 73 88 291 807
Size: 73 15 203 516
Fragments arranged by size:
516 203 73 15
NarI GG'CG_CC
Cuts at: 0 67 241 807
Size: 67 174 566
Fragments arranged by size:
566 174 67
NcoI C'CATG_G
Cuts at: 0 591 807
Size: 591 216
PasI CC'CwG_GG
Cuts at: 0 680 807
Size: 680 127
PfoI T'CCnGG_A
Cuts at: 0 191 296 600 807
Size: 191 105 304 207
Fragments arranged by size:
304 207 191 105
PpuMI rG'GwC_Cy
Cuts at: 0 189 664 807
Size: 189 475 143
Fragments arranged by size:
475 189 143
PspOMI G'GGCC_C
Cuts at: 0 305 807
Size: 305 502
PstI C_TGCA'G
Cuts at: 0 49 807
Size: 49 758
SacII CC_GC'GG
Cuts at: 0 74 807
Size: 74 733
SanDI GG'GwC_CC
Cuts at: 0 664 807
Size: 664 143
ScaI AGT'ACT
Cuts at: 0 544 807
Size: 544 263
SfcI C'TryA_G
Cuts at: 0 45 807
Size: 45 762
SfoI GGC'GCC
Cuts at: 0 68 242 807
Size: 68 174 565
Fragments arranged by size:
565 174 68
StuI AGG'CCT
Cuts at: 0 344 807
Size: 344 463
StyI C'CwwG_G
Cuts at: 0 591 612 807
Size: 591 21 195
Fragments arranged by size:
591 195 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 378 672 807
Size: 378 294 135
Fragments arranged by size:
378 294 135
TatI w'GTAC_w
Cuts at: 0 542 807
Size: 542 265
Tth111I GACn'n_nGTC
Cuts at: 0 280 807
Size: 280 527
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaI BamHI BanI
BanII BbeI BbsI BcgI Bme1580I BmrI BpmI Bpu10I
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI Bsp1286I
BspMI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI
BtsI EaeI Eco57MI EcoNI EcoO109I FspI HaeII KasI
MscI MslI MspA1I NarI NcoI PasI PfoI PpuMI
PspOMI PstI SacII SanDI ScaI SfcI SfoI StuI
StyI TaqII TatI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbvCI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BpuEI BsaBI BsaWI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SalI SapI SbfI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI