Restriction map for
pFN21AE1754
(Linear) (Six-base) MAPSORT of: KIEE1754.seq Check: 3774 from: 1 to: 393
KIEE1754.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 51 393
Size: 51 342
AlwNI CAG_nnn'CTG
Cuts at: 0 123 393
Size: 123 270
ApaI G_GGCC'C
Cuts at: 0 216 231 393
Size: 216 15 162
Fragments arranged by size:
216 162 15
ApoI r'AATT_y
Cuts at: 0 4 393
Size: 4 389
AvaI C'yCGr_G
Cuts at: 0 147 183 215 376 393
Size: 147 36 32 161 17
Fragments arranged by size:
161 147 36 32 17
BanI G'GyrC_C
Cuts at: 0 42 299 393
Size: 42 257 94
Fragments arranged by size:
257 94 42
BanII G_rGCy'C
Cuts at: 0 156 216 231 375 389 393
Size: 156 60 15 144 14 4
Fragments arranged by size:
156 144 60 15 14 4
BbvCI CC'TCA_GC
Cuts at: 0 92 393
Size: 92 301
BglI GCCn_nnn'nGGC
Cuts at: 0 151 393
Size: 151 242
Bme1580I G_kGCm'C
Cuts at: 0 47 140 216 231 393
Size: 47 93 76 15 162
Fragments arranged by size:
162 93 76 47 15
BmgBI CAC'GTC
Cuts at: 0 65 393
Size: 65 328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 336 393
Size: 145 191 57
Fragments arranged by size:
191 145 57
Bpu10I CC'TnA_GC
Cuts at: 0 92 393
Size: 92 301
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 393
Size: 193 30 170
Fragments arranged by size:
193 170 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 301 393
Size: 214 87 92
Fragments arranged by size:
214 92 87
BseYI C'CCAG_C
Cuts at: 0 139 230 393
Size: 139 91 163
Fragments arranged by size:
163 139 91
Bsp1286I G_dGCh'C
Cuts at: 0 47 140 156 216 231 375 389 393
Size: 47 93 16 60 15 144 14 4
Fragments arranged by size:
144 93 60 47 16 15 14 4
BsrBI CCG'CTC
Cuts at: 0 273 393
Size: 273 120
BsrGI T'GTAC_A
Cuts at: 0 326 393
Size: 326 67
BstXI CCAn_nnnn'nTGG
Cuts at: 0 85 393
Size: 85 308
BtgI C'CryG_G
Cuts at: 0 192 393
Size: 192 201
DraIII CAC_nnn'GTG
Cuts at: 0 65 393
Size: 65 328
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 127 255 262 291 393
Size: 127 128 7 29 102
Fragments arranged by size:
128 127 102 29 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 336 393
Size: 145 191 57
Fragments arranged by size:
191 145 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 357 393
Size: 357 36
EcoO109I rG'GnC_Cy
Cuts at: 0 69 227 321 393
Size: 69 158 94 72
Fragments arranged by size:
158 94 72 69
HaeII r_GCGC'y
Cuts at: 0 146 393
Size: 146 247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 72 104 393
Size: 72 32 289
Fragments arranged by size:
289 72 32
MslI CAynn'nnrTG
Cuts at: 0 33 393
Size: 33 360
MspA1I CmG'CkG
Cuts at: 0 194 234 297 393
Size: 194 40 63 96
Fragments arranged by size:
194 96 63 40
NspI r_CATG'y
Cuts at: 0 32 393
Size: 32 361
PasI CC'CwG_GG
Cuts at: 0 47 359 393
Size: 47 312 34
Fragments arranged by size:
312 47 34
PflMI CCAn_nnn'nTGG
Cuts at: 0 65 393
Size: 65 328
PpuMI rG'GwC_Cy
Cuts at: 0 69 321 393
Size: 69 252 72
Fragments arranged by size:
252 72 69
PshAI GACnn'nnGTC
Cuts at: 0 67 393
Size: 67 326
PspOMI G'GGCC_C
Cuts at: 0 212 227 393
Size: 212 15 166
Fragments arranged by size:
212 166 15
PvuII CAG'CTG
Cuts at: 0 234 393
Size: 234 159
SacII CC_GC'GG
Cuts at: 0 195 393
Size: 195 198
SanDI GG'GwC_CC
Cuts at: 0 69 393
Size: 69 324
SmaI CCC'GGG
Cuts at: 0 149 185 217 393
Size: 149 36 32 176
Fragments arranged by size:
176 149 36 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 393
Size: 187 206
TatI w'GTAC_w
Cuts at: 0 326 393
Size: 326 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 393
Size: 193 32 168
Fragments arranged by size:
193 168 32
XmaI C'CCGG_G
Cuts at: 0 147 183 215 393
Size: 147 36 32 178
Fragments arranged by size:
178 147 36 32
Enzymes that do cut:
AlfI AlwNI ApaI ApoI AvaI BanI BanII BbvCI
BglI Bme1580I BmgBI BpmI Bpu10I BsaXI BseRI BseYI
Bsp1286I BsrBI BsrGI BstXI BtgI DraIII EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I MslI MspA1I NspI PasI
PflMI PpuMI PshAI PspOMI PvuII SacII SanDI SmaI
TaqII TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI PstI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI