Restriction map for
pFN21AE1753
(Linear) (Six-base) MAPSORT of: KIEE1753.seq Check: 7399 from: 1 to: 780
KIEE1753.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 651 780
Size: 651 129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 451 769 780
Size: 151 300 318 11
Fragments arranged by size:
318 300 151 11
AfeI AGC'GCT
Cuts at: 0 453 780
Size: 453 327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 407 439 780
Size: 407 32 341
Fragments arranged by size:
407 341 32
AlwNI CAG_nnn'CTG
Cuts at: 0 747 780
Size: 747 33
ApoI r'AATT_y
Cuts at: 0 670 780
Size: 670 110
BamHI G'GATC_C
Cuts at: 0 173 780
Size: 173 607
BanI G'GyrC_C
Cuts at: 0 731 780
Size: 731 49
BbeI G_GCGC'C
Cuts at: 0 735 780
Size: 735 45
BclI T'GATC_A
Cuts at: 0 153 780
Size: 153 627
BlpI GC'TnA_GC
Cuts at: 0 740 780
Size: 740 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 21 297 780
Size: 21 276 483
Fragments arranged by size:
483 276 21
Bpu10I CC'TnA_GC
Cuts at: 0 38 780
Size: 38 742
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 616 780
Size: 220 396 164
Fragments arranged by size:
396 220 164
BsaHI Gr'CG_yC
Cuts at: 0 732 780
Size: 732 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 582 612 780
Size: 582 30 168
Fragments arranged by size:
582 168 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 590 780
Size: 590 190
BseYI C'CCAG_C
Cuts at: 0 735 780
Size: 735 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 780
Size: 421 359
BsiHKAI G_wGCw'C
Cuts at: 0 44 236 632 780
Size: 44 192 396 148
Fragments arranged by size:
396 192 148 44
Bsp1286I G_dGCh'C
Cuts at: 0 44 236 632 780
Size: 44 192 396 148
Fragments arranged by size:
396 192 148 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 651 780
Size: 651 129
BsrDI GCAATG_nn'
Cuts at: 0 62 780
Size: 62 718
BssSI C'ACGA_G
Cuts at: 0 539 780
Size: 539 241
BstYI r'GATC_y
Cuts at: 0 173 780
Size: 173 607
Bsu36I CC'TnA_GG
Cuts at: 0 169 780
Size: 169 611
BtsI GCAGTG_nn'
Cuts at: 0 236 780
Size: 236 544
EaeI y'GGCC_r
Cuts at: 0 419 780
Size: 419 361
EarI CTCTTCn'nnn_
Cuts at: 0 608 780
Size: 608 172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 451 769 780
Size: 151 300 318 11
Fragments arranged by size:
318 300 151 11
EcoO109I rG'GnC_Cy
Cuts at: 0 244 780
Size: 244 536
HaeII r_GCGC'y
Cuts at: 0 81 455 735 780
Size: 81 374 280 45
Fragments arranged by size:
374 280 81 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 582 614 780
Size: 67 32 483 32 166
Fragments arranged by size:
483 166 67 32 32
HincII GTy'rAC
Cuts at: 0 93 780
Size: 93 687
HindIII A'AGCT_T
Cuts at: 0 767 780
Size: 767 13
KasI G'GCGC_C
Cuts at: 0 731 780
Size: 731 49
NarI GG'CG_CC
Cuts at: 0 732 780
Size: 732 48
PpuMI rG'GwC_Cy
Cuts at: 0 244 780
Size: 244 536
PstI C_TGCA'G
Cuts at: 0 348 780
Size: 348 432
SfcI C'TryA_G
Cuts at: 0 344 780
Size: 344 436
SfoI GGC'GCC
Cuts at: 0 733 780
Size: 733 47
SmlI C'TyrA_G
Cuts at: 0 235 631 780
Size: 235 396 149
Fragments arranged by size:
396 235 149
StyI C'CwwG_G
Cuts at: 0 68 780
Size: 68 712
Enzymes that do cut:
AarI AcuI AfeI AloI AlwNI ApoI BamHI BanI
BbeI BclI BlpI BmrI Bpu10I BpuEI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BssSI
BstYI Bsu36I BtsI EaeI EarI Eco57MI EcoO109I HaeII
Hin4I HincII HindIII KasI NarI PpuMI PstI SfcI
SfoI SmlI StyI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanII BbsI BbvCI BcgI BciVI BfrBI
BglI BglII Bme1580I BmgBI BmtI BplI BpmI BsaI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI