Restriction map for
pFN21AE1751
(Linear) (Six-base) MAPSORT of: KIEE1751.seq Check: 5444 from: 1 to: 762
KIEE1751.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 506 762
Size: 506 256
AccI GT'mk_AC
Cuts at: 0 419 762
Size: 419 343
AleI CACnn'nnGTG
Cuts at: 0 617 762
Size: 617 145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 635 762
Size: 635 127
AlwNI CAG_nnn'CTG
Cuts at: 0 507 633 762
Size: 507 126 129
Fragments arranged by size:
507 129 126
ApaI G_GGCC'C
Cuts at: 0 90 317 762
Size: 90 227 445
Fragments arranged by size:
445 227 90
AvaI C'yCGr_G
Cuts at: 0 201 220 762
Size: 201 19 542
Fragments arranged by size:
542 201 19
BanI G'GyrC_C
Cuts at: 0 691 762
Size: 691 71
BanII G_rGCy'C
Cuts at: 0 90 174 317 472 762
Size: 90 84 143 155 290
Fragments arranged by size:
290 155 143 90 84
BbeI G_GCGC'C
Cuts at: 0 695 762
Size: 695 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 132 420 762
Size: 132 288 342
Fragments arranged by size:
342 288 132
BglI GCCn_nnn'nGGC
Cuts at: 0 347 535 762
Size: 347 188 227
Fragments arranged by size:
347 227 188
Bme1580I G_kGCm'C
Cuts at: 0 90 317 666 762
Size: 90 227 349 96
Fragments arranged by size:
349 227 96 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 762
Size: 434 328
Bpu10I CC'TnA_GC
Cuts at: 0 319 370 471 762
Size: 319 51 101 291
Fragments arranged by size:
319 291 101 51
BsaHI Gr'CG_yC
Cuts at: 0 638 692 762
Size: 638 54 70
Fragments arranged by size:
638 70 54
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 555 762
Size: 555 207
BseYI C'CCAG_C
Cuts at: 0 520 732 762
Size: 520 212 30
Fragments arranged by size:
520 212 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 595 762
Size: 478 117 167
Fragments arranged by size:
478 167 117
Bsp1286I G_dGCh'C
Cuts at: 0 90 174 317 472 666 762
Size: 90 84 143 155 194 96
Fragments arranged by size:
194 155 143 96 90 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 506 560 762
Size: 266 240 54 202
Fragments arranged by size:
266 240 202 54
BsrFI r'CCGG_y
Cuts at: 0 244 460 622 762
Size: 244 216 162 140
Fragments arranged by size:
244 216 162 140
BstAPI GCAn_nnn'nTGC
Cuts at: 0 507 670 762
Size: 507 163 92
Fragments arranged by size:
507 163 92
BstEII G'GTnAC_C
Cuts at: 0 717 762
Size: 717 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 702 762
Size: 702 60
BtgI C'CryG_G
Cuts at: 0 40 191 545 762
Size: 40 151 354 217
Fragments arranged by size:
354 217 151 40
BtsI GCAGTG_nn'
Cuts at: 0 505 762
Size: 505 257
ClaI AT'CG_AT
Cuts at: 0 308 762
Size: 308 454
EaeI y'GGCC_r
Cuts at: 0 194 404 458 624 762
Size: 194 210 54 166 138
Fragments arranged by size:
210 194 166 138 54
EarI CTCTTCn'nnn_
Cuts at: 0 715 762
Size: 715 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 762
Size: 434 328
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 762
Size: 262 500
EcoO109I rG'GnC_Cy
Cuts at: 0 87 217 314 366 762
Size: 87 130 97 52 396
Fragments arranged by size:
396 130 97 87 52
FspI TGC'GCA
Cuts at: 0 278 762
Size: 278 484
HaeII r_GCGC'y
Cuts at: 0 633 695 762
Size: 633 62 67
Fragments arranged by size:
633 67 62
KasI G'GCGC_C
Cuts at: 0 691 762
Size: 691 71
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 762
Size: 737 25
MscI TGG'CCA
Cuts at: 0 406 762
Size: 406 356
MslI CAynn'nnrTG
Cuts at: 0 617 762
Size: 617 145
MspA1I CmG'CkG
Cuts at: 0 193 282 762
Size: 193 89 480
Fragments arranged by size:
480 193 89
NaeI GCC'GGC
Cuts at: 0 246 462 624 762
Size: 246 216 162 138
Fragments arranged by size:
246 216 162 138
NarI GG'CG_CC
Cuts at: 0 692 762
Size: 692 70
NgoMIV G'CCGG_C
Cuts at: 0 244 460 622 762
Size: 244 216 162 140
Fragments arranged by size:
244 216 162 140
NspI r_CATG'y
Cuts at: 0 273 762
Size: 273 489
PasI CC'CwG_GG
Cuts at: 0 155 687 762
Size: 155 532 75
Fragments arranged by size:
532 155 75
PflMI CCAn_nnn'nTGG
Cuts at: 0 633 762
Size: 633 129
PpuMI rG'GwC_Cy
Cuts at: 0 217 762
Size: 217 545
PspOMI G'GGCC_C
Cuts at: 0 86 313 762
Size: 86 227 449
Fragments arranged by size:
449 227 86
PstI C_TGCA'G
Cuts at: 0 503 762
Size: 503 259
PvuII CAG'CTG
Cuts at: 0 282 762
Size: 282 480
SacII CC_GC'GG
Cuts at: 0 194 762
Size: 194 568
SanDI GG'GwC_CC
Cuts at: 0 217 762
Size: 217 545
SfcI C'TryA_G
Cuts at: 0 499 762
Size: 499 263
SfoI GGC'GCC
Cuts at: 0 693 762
Size: 693 69
StuI AGG'CCT
Cuts at: 0 341 762
Size: 341 421
StyI C'CwwG_G
Cuts at: 0 82 407 762
Size: 82 325 355
Fragments arranged by size:
355 325 82
Enzymes that do cut:
AarI AccI AleI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BglI Bme1580I BpmI Bpu10I BsaHI
BseRI BseYI BsgI Bsp1286I BspMI BsrFI BstAPI BstEII
BstXI BtgI BtsI ClaI EaeI EarI Eco57MI EcoNI
EcoO109I FspI HaeII KasI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PflMI PpuMI PspOMI
PstI PvuII SacII SanDI SfcI SfoI StuI StyI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstYI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI