Restriction map for
pFN21AE1750
(Linear) (Six-base) MAPSORT of: KIEE1750.seq Check: 4476 from: 1 to: 759
KIEE1750.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 541 759
Size: 541 218
AfeI AGC'GCT
Cuts at: 0 574 759
Size: 574 185
AleI CACnn'nnGTG
Cuts at: 0 718 759
Size: 718 41
ApaI G_GGCC'C
Cuts at: 0 382 631 759
Size: 382 249 128
Fragments arranged by size:
382 249 128
AvaI C'yCGr_G
Cuts at: 0 652 669 703 759
Size: 652 17 34 56
Fragments arranged by size:
652 56 34 17
AvrII C'CTAG_G
Cuts at: 0 36 759
Size: 36 723
BamHI G'GATC_C
Cuts at: 0 74 759
Size: 74 685
BanI G'GyrC_C
Cuts at: 0 57 426 559 759
Size: 57 369 133 200
Fragments arranged by size:
369 200 133 57
BanII G_rGCy'C
Cuts at: 0 269 382 631 676 759
Size: 269 113 249 45 83
Fragments arranged by size:
269 249 113 83 45
BbeI G_GCGC'C
Cuts at: 0 61 563 759
Size: 61 502 196
Fragments arranged by size:
502 196 61
BglI GCCn_nnn'nGGC
Cuts at: 0 55 759
Size: 55 704
BlpI GC'TnA_GC
Cuts at: 0 184 759
Size: 184 575
Bme1580I G_kGCm'C
Cuts at: 0 305 382 631 759
Size: 305 77 249 128
Fragments arranged by size:
305 249 128 77
BmgBI CAC'GTC
Cuts at: 0 288 423 447 759
Size: 288 135 24 312
Fragments arranged by size:
312 288 135 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 385 759
Size: 385 374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 759
Size: 223 536
BsaAI yAC'GTr
Cuts at: 0 504 759
Size: 504 255
BsaHI Gr'CG_yC
Cuts at: 0 58 121 158 560 759
Size: 58 63 37 402 199
Fragments arranged by size:
402 199 63 58 37
BseYI C'CCAG_C
Cuts at: 0 8 455 759
Size: 8 447 304
Fragments arranged by size:
447 304 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 247 759
Size: 148 99 512
Fragments arranged by size:
512 148 99
BsiEI CG_ry'CG
Cuts at: 0 47 215 759
Size: 47 168 544
Fragments arranged by size:
544 168 47
BsiHKAI G_wGCw'C
Cuts at: 0 293 509 653 759
Size: 293 216 144 106
Fragments arranged by size:
293 216 144 106
Bsp1286I G_dGCh'C
Cuts at: 0 269 293 305 382 509 631 653 676
Size: 269 24 12 77 127 122 22 23
Cuts at: 676 759
Size: 83
Fragments arranged by size:
269 127 122 83 77 24 23 22
12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 219 541 759
Size: 219 322 218
Fragments arranged by size:
322 219 218
BsrBI CCG'CTC
Cuts at: 0 43 759
Size: 43 716
BsrFI r'CCGG_y
Cuts at: 0 451 539 759
Size: 451 88 220
Fragments arranged by size:
451 220 88
BssHII G'CGCG_C
Cuts at: 0 140 242 759
Size: 140 102 517
Fragments arranged by size:
517 140 102
BstAPI GCAn_nnn'nTGC
Cuts at: 0 180 504 759
Size: 180 324 255
Fragments arranged by size:
324 255 180
BstEII G'GTnAC_C
Cuts at: 0 663 759
Size: 663 96
BstYI r'GATC_y
Cuts at: 0 74 707 759
Size: 74 633 52
Fragments arranged by size:
633 74 52
BtgI C'CryG_G
Cuts at: 0 66 759
Size: 66 693
BtsI GCAGTG_nn'
Cuts at: 0 181 195 305 759
Size: 181 14 110 454
Fragments arranged by size:
454 181 110 14
EaeI y'GGCC_r
Cuts at: 0 44 212 449 759
Size: 44 168 237 310
Fragments arranged by size:
310 237 168 44
EagI C'GGCC_G
Cuts at: 0 44 212 759
Size: 44 168 547
Fragments arranged by size:
547 168 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 184 759
Size: 184 575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 759
Size: 223 536
EcoO109I rG'GnC_Cy
Cuts at: 0 379 627 759
Size: 379 248 132
Fragments arranged by size:
379 248 132
HaeII r_GCGC'y
Cuts at: 0 18 61 279 563 576 759
Size: 18 43 218 284 13 183
Fragments arranged by size:
284 218 183 43 18 13
HincII GTy'rAC
Cuts at: 0 468 759
Size: 468 291
KasI G'GCGC_C
Cuts at: 0 57 559 759
Size: 57 502 200
Fragments arranged by size:
502 200 57
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 341 759
Size: 52 289 418
Fragments arranged by size:
418 289 52
MslI CAynn'nnrTG
Cuts at: 0 718 759
Size: 718 41
MspA1I CmG'CkG
Cuts at: 0 8 132 357 608 633 759
Size: 8 124 225 251 25 126
Fragments arranged by size:
251 225 126 124 25 8
NaeI GCC'GGC
Cuts at: 0 453 759
Size: 453 306
NarI GG'CG_CC
Cuts at: 0 58 560 759
Size: 58 502 199
Fragments arranged by size:
502 199 58
NgoMIV G'CCGG_C
Cuts at: 0 451 759
Size: 451 308
NotI GC'GGCC_GC
Cuts at: 0 44 759
Size: 44 715
PmlI CAC'GTG
Cuts at: 0 504 759
Size: 504 255
PspOMI G'GGCC_C
Cuts at: 0 378 627 759
Size: 378 249 132
Fragments arranged by size:
378 249 132
PspXI vC'TCGA_Gb
Cuts at: 0 652 759
Size: 652 107
PstI C_TGCA'G
Cuts at: 0 500 759
Size: 500 259
PvuII CAG'CTG
Cuts at: 0 8 132 357 608 759
Size: 8 124 225 251 151
Fragments arranged by size:
251 225 151 124 8
SfcI C'TryA_G
Cuts at: 0 352 496 759
Size: 352 144 263
Fragments arranged by size:
352 263 144
SfoI GGC'GCC
Cuts at: 0 59 561 759
Size: 59 502 198
Fragments arranged by size:
502 198 59
SmaI CCC'GGG
Cuts at: 0 705 759
Size: 705 54
SmlI C'TyrA_G
Cuts at: 0 652 759
Size: 652 107
StyI C'CwwG_G
Cuts at: 0 36 759
Size: 36 723
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 168 759
Size: 168 591
Tth111I GACn'n_nGTC
Cuts at: 0 748 759
Size: 748 11
XhoI C'TCGA_G
Cuts at: 0 652 759
Size: 652 107
XmaI C'CCGG_G
Cuts at: 0 703 759
Size: 703 56
Enzymes that do cut:
AarI AfeI AleI ApaI AvaI AvrII BamHI BanI
BanII BbeI BglI BlpI Bme1580I BmgBI BmrI BpmI
BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssHII BstAPI BstEII BstYI BtgI BtsI
EaeI EagI EciI Eco57MI EcoO109I HaeII HincII KasI
MmeI MslI MspA1I NaeI NarI NgoMIV NotI PmlI
PspOMI PspXI PstI PvuII SfcI SfoI SmaI SmlI
StyI TaqII Tth111I XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI BaeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
KpnI MfeI MluI MscI NcoI NdeI NheI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI XbaI XcmI XmnI ZraI