(Linear) (Six-base) MAPSORT of: KIEE1744.seq  Check: 3955  from: 1  to: 735



KIEE1744.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:46  ..

ApoI r'AATT_y

Cuts at:      0     292     341     433     735 
   Size:        292      49      92     302

  Fragments arranged by size: 

                 302     292      92      49
 
AseI AT'TA_AT

Cuts at:      0     473     735 
   Size:        473     262
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      35      68     735 
   Size:         35      33     667

  Fragments arranged by size: 

                 667      35      33
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      36     278     735 
   Size:         36     242     457

  Fragments arranged by size: 

                 457     242      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     670     735 
   Size:        670      65
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     449     479     735 
   Size:        449      30     256

  Fragments arranged by size: 

                 449     256      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     470     735 
   Size:        470     265
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     466     735 
   Size:        466     269
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      76     111     735 
   Size:         76      35     624

  Fragments arranged by size: 

                 624      76      35
 
EarI CTCTTCn'nnn_

Cuts at:      0      26     735 
   Size:         26     709
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     589     735 
   Size:        589     146
 
NspI r_CATG'y

Cuts at:      0     604     735 
   Size:        604     131
 
PfoI T'CCnGG_A

Cuts at:      0     345     735 
   Size:        345     390
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     269     301     735 
   Size:        269      32     434

  Fragments arranged by size: 

                 434     269      32
 
ScaI AGT'ACT

Cuts at:      0      37      68     735 
   Size:         37      31     667

  Fragments arranged by size: 

                 667      37      31
 
SmlI C'TyrA_G

Cuts at:      0     649     735 
   Size:        649      86
 
SspI AAT'ATT

Cuts at:      0     426     735 
   Size:        426     309
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      87     735 
   Size:         87     648
 
TatI w'GTAC_w

Cuts at:      0      35      66     389     735 
   Size:         35      31     323     346

  Fragments arranged by size: 

                 346     323      35      31
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     327     359     735 
   Size:        327      32     376

  Fragments arranged by size: 

                 376     327      32
 

 Enzymes that do cut:

     ApoI     AseI     BaeI    BciVI    BpuEI    BsaXI    BseRI   BstAPI
    CspCI     EarI     MmeI     NspI     PfoI     PsrI     ScaI     SmlI
     SspI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI