Restriction map for
pFN21AE1743
(Linear) (Six-base) MAPSORT of: KIEE1743.seq Check: 7966 from: 1 to: 735
KIEE1743.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 690 735
Size: 604 86 45
Fragments arranged by size:
604 86 45
AlwNI CAG_nnn'CTG
Cuts at: 0 495 735
Size: 495 240
ApaI G_GGCC'C
Cuts at: 0 168 224 260 735
Size: 168 56 36 475
Fragments arranged by size:
475 168 56 36
AvaI C'yCGr_G
Cuts at: 0 11 288 735
Size: 11 277 447
Fragments arranged by size:
447 277 11
BanI G'GyrC_C
Cuts at: 0 301 735
Size: 301 434
BanII G_rGCy'C
Cuts at: 0 168 224 260 279 590 688 735
Size: 168 56 36 19 311 98 47
Fragments arranged by size:
311 168 98 56 47 36 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 117 696 735
Size: 117 579 39
Fragments arranged by size:
579 117 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 437 471 735
Size: 437 34 264
Fragments arranged by size:
437 264 34
Bme1580I G_kGCm'C
Cuts at: 0 168 224 260 735
Size: 168 56 36 475
Fragments arranged by size:
475 168 56 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 495 735
Size: 495 240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 519 735
Size: 124 395 216
Fragments arranged by size:
395 216 124
BsaI GGTCTCn'nnnn_
Cuts at: 0 197 735
Size: 197 538
BsaWI w'CCGG_w
Cuts at: 0 176 735
Size: 176 559
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 519 522 735
Size: 519 3 213
Fragments arranged by size:
519 213 3
BseYI C'CCAG_C
Cuts at: 0 259 735
Size: 259 476
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 735
Size: 441 294
BsiEI CG_ry'CG
Cuts at: 0 447 735
Size: 447 288
BsiHKAI G_wGCw'C
Cuts at: 0 29 84 735
Size: 29 55 651
Fragments arranged by size:
651 55 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 531 735
Size: 531 204
Bsp1286I G_dGCh'C
Cuts at: 0 29 84 168 224 260 279 590 688
Size: 29 55 84 56 36 19 311 98
Cuts at: 688 735
Size: 47
Fragments arranged by size:
311 98 84 56 55 47 36 29
19
BspEI T'CCGG_A
Cuts at: 0 176 735
Size: 176 559
BstEII G'GTnAC_C
Cuts at: 0 60 634 735
Size: 60 574 101
Fragments arranged by size:
574 101 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 735
Size: 48 687
BtgI C'CryG_G
Cuts at: 0 279 735
Size: 279 456
EaeI y'GGCC_r
Cuts at: 0 38 130 444 735
Size: 38 92 314 291
Fragments arranged by size:
314 291 92 38
EagI C'GGCC_G
Cuts at: 0 444 735
Size: 444 291
EarI CTCTTCn'nnn_
Cuts at: 0 595 735
Size: 595 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 342 378 735
Size: 342 36 357
Fragments arranged by size:
357 342 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 690 735
Size: 604 86 45
Fragments arranged by size:
604 86 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 534 735
Size: 534 201
EcoO109I rG'GnC_Cy
Cuts at: 0 7 164 165 221 256 735
Size: 7 157 1 56 35 479
Fragments arranged by size:
479 157 56 35 7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 606 735
Size: 606 129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 735
Size: 573 162
MscI TGG'CCA
Cuts at: 0 40 735
Size: 40 695
MspA1I CmG'CkG
Cuts at: 0 263 624 710 735
Size: 263 361 86 25
Fragments arranged by size:
361 263 86 25
PasI CC'CwG_GG
Cuts at: 0 253 558 735
Size: 253 305 177
Fragments arranged by size:
305 253 177
PpuMI rG'GwC_Cy
Cuts at: 0 7 735
Size: 7 728
PspOMI G'GGCC_C
Cuts at: 0 164 220 256 735
Size: 164 56 36 479
Fragments arranged by size:
479 164 56 36
PstI C_TGCA'G
Cuts at: 0 629 735
Size: 629 106
PvuII CAG'CTG
Cuts at: 0 624 735
Size: 624 111
SanDI GG'GwC_CC
Cuts at: 0 7 735
Size: 7 728
SapI GCTCTTCn'nnn_
Cuts at: 0 595 735
Size: 595 140
SexAI A'CCwGG_T
Cuts at: 0 638 735
Size: 638 97
SfcI C'TryA_G
Cuts at: 0 625 735
Size: 625 110
SmaI CCC'GGG
Cuts at: 0 13 290 735
Size: 13 277 445
Fragments arranged by size:
445 277 13
SmlI C'TyrA_G
Cuts at: 0 139 534 735
Size: 139 395 201
Fragments arranged by size:
395 201 139
StuI AGG'CCT
Cuts at: 0 137 194 735
Size: 137 57 541
Fragments arranged by size:
541 137 57
StyI C'CwwG_G
Cuts at: 0 189 305 735
Size: 189 116 430
Fragments arranged by size:
430 189 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 735
Size: 79 656
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 8 40 735
Size: 8 32 695
Fragments arranged by size:
695 32 8
Tth111I GACn'n_nGTC
Cuts at: 0 403 640 700 735
Size: 403 237 60 35
Fragments arranged by size:
403 237 60 35
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 214 735
Size: 214 521
XmaI C'CCGG_G
Cuts at: 0 11 288 735
Size: 11 277 447
Fragments arranged by size:
447 277 11
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanI BanII BbsI BcgI
Bme1580I BmrI BpuEI BsaI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BstEII BstXI BtgI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI
MmeI MscI MspA1I PasI PpuMI PspOMI PstI PvuII
SanDI SapI SexAI SfcI SmaI SmlI StuI StyI
TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpmI Bpu10I BsaAI BsaBI BsaHI BsaXI BsiWI BsmI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI XbaI XhoI XmnI ZraI