(Linear) (Six-base) MAPSORT of: KIEE1741.seq  Check: 1651  from: 1  to: 732



KIEE1741.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:46  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     263     732 
   Size:        263     469
 
AlwNI CAG_nnn'CTG

Cuts at:      0     689     732 
   Size:        689      43
 
ApoI r'AATT_y

Cuts at:      0     180     732 
   Size:        180     552
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     393     426     732 
   Size:        393      33     306

  Fragments arranged by size: 

                 393     306      33
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     394     732 
   Size:        394     338
 
BlpI GC'TnA_GC

Cuts at:      0     651     732 
   Size:        651      81
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     341     732 
   Size:        341     391
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     512     711     732 
   Size:        352     160     199      21

  Fragments arranged by size: 

                 352     199     160      21
 
Bpu10I CC'TnA_GC

Cuts at:      0     583     732 
   Size:        583     149
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     561     732 
   Size:        561     171
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     439     732 
   Size:        439     293
 
BsiEI CG_ry'CG

Cuts at:      0      11     732 
   Size:         11     721
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      62     357     732 
   Size:         62     295     375

  Fragments arranged by size: 

                 375     295      62
 
BstEII G'GTnAC_C

Cuts at:      0     226     732 
   Size:        226     506
 
BstYI r'GATC_y

Cuts at:      0     412     732 
   Size:        412     320
 
BtgI C'CryG_G

Cuts at:      0     408     638     732 
   Size:        408     230      94

  Fragments arranged by size: 

                 408     230      94
 
BtsI GCAGTG_nn'

Cuts at:      0     210     732 
   Size:        210     522
 
DraIII CAC_nnn'GTG

Cuts at:      0     349     732 
   Size:        349     383
 
EaeI y'GGCC_r

Cuts at:      0       8     732 
   Size:          8     724
 
EagI C'GGCC_G

Cuts at:      0       8     732 
   Size:          8     724
 
EarI CTCTTCn'nnn_

Cuts at:      0     230     604     732 
   Size:        230     374     128

  Fragments arranged by size: 

                 374     230     128
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     516     732 
   Size:        516     216
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     512     711     732 
   Size:        352     160     199      21

  Fragments arranged by size: 

                 352     199     160      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      79     111     732 
   Size:         79      32     621

  Fragments arranged by size: 

                 621      79      32
 
MspA1I CmG'CkG

Cuts at:      0       7     243     732 
   Size:          7     236     489

  Fragments arranged by size: 

                 489     236       7
 
NcoI C'CATG_G

Cuts at:      0     638     732 
   Size:        638      94
 
NotI GC'GGCC_GC

Cuts at:      0       8     732 
   Size:          8     724
 
NruI TCG'CGA

Cuts at:      0      40     732 
   Size:         40     692
 
PfoI T'CCnGG_A

Cuts at:      0      70     732 
   Size:         70     662
 
PstI C_TGCA'G

Cuts at:      0     368     732 
   Size:        368     364
 
PvuII CAG'CTG

Cuts at:      0     243     732 
   Size:        243     489
 
SapI GCTCTTCn'nnn_

Cuts at:      0     230     732 
   Size:        230     502
 
SfcI C'TryA_G

Cuts at:      0     364     732 
   Size:        364     368
 
StyI C'CwwG_G

Cuts at:      0     638     732 
   Size:        638      94
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     360     648     732 
   Size:        360     288      84

  Fragments arranged by size: 

                 360     288      84
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     366     398     669     701     732 
   Size:        366      32     271      32      31

  Fragments arranged by size: 

                 366     271      32      32      31
 
Tth111I GACn'n_nGTC

Cuts at:      0     211     732 
   Size:        211     521
 

 Enzymes that do cut:

     AlfI    AlwNI     ApoI     BaeI    BciVI     BlpI     BmrI     BpmI
   Bpu10I     BsaI    BseRI    BsiEI    BspMI   BstEII    BstYI     BtgI
     BtsI   DraIII     EaeI     EagI     EarI     EciI  Eco57MI    Hin4I
   MspA1I     NcoI     NotI     NruI     PfoI     PstI    PvuII     SapI
     SfcI     StyI    TaqII     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI     DrdI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI