Restriction map for
pFN21AE1737
(Linear) (Six-base) MAPSORT of: KIEE1737.seq Check: 9647 from: 1 to: 729
KIEE1737.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
Acc65I G'GTAC_C
Cuts at: 0 281 729
Size: 281 448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 529 729
Size: 276 253 200
Fragments arranged by size:
276 253 200
AfeI AGC'GCT
Cuts at: 0 38 729
Size: 38 691
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 574 606 729
Size: 574 32 123
Fragments arranged by size:
574 123 32
AvaI C'yCGr_G
Cuts at: 0 6 729
Size: 6 723
BamHI G'GATC_C
Cuts at: 0 264 729
Size: 264 465
BanI G'GyrC_C
Cuts at: 0 216 281 448 729
Size: 216 65 167 281
Fragments arranged by size:
281 216 167 65
BanII G_rGCy'C
Cuts at: 0 7 729
Size: 7 722
BbsI GAAGACnn'nnnn_
Cuts at: 0 517 729
Size: 517 212
BbvCI CC'TCA_GC
Cuts at: 0 490 729
Size: 490 239
Bme1580I G_kGCm'C
Cuts at: 0 453 729
Size: 453 276
BmrI ACTGGGnnnn_n'
Cuts at: 0 369 729
Size: 369 360
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 505 729
Size: 505 224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 619 729
Size: 280 339 110
Fragments arranged by size:
339 280 110
Bpu10I CC'TnA_GC
Cuts at: 0 490 729
Size: 490 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 535 729
Size: 535 194
BsaAI yAC'GTr
Cuts at: 0 699 729
Size: 699 30
BsaWI w'CCGG_w
Cuts at: 0 690 729
Size: 690 39
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 691 729
Size: 691 38
BseYI C'CCAG_C
Cuts at: 0 253 452 729
Size: 253 199 277
Fragments arranged by size:
277 253 199
BsiHKAI G_wGCw'C
Cuts at: 0 7 419 729
Size: 7 412 310
Fragments arranged by size:
412 310 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 419 453 729
Size: 7 412 34 276
Fragments arranged by size:
412 276 34 7
BsrDI GCAATG_nn'
Cuts at: 0 338 729
Size: 338 391
BstXI CCAn_nnnn'nTGG
Cuts at: 0 345 729
Size: 345 384
BstYI r'GATC_y
Cuts at: 0 264 729
Size: 264 465
BtgI C'CryG_G
Cuts at: 0 344 729
Size: 344 385
EaeI y'GGCC_r
Cuts at: 0 193 729
Size: 193 536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 223 729
Size: 223 506
EcoICRI GAG'CTC
Cuts at: 0 5 729
Size: 5 724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 280 529 619 729
Size: 276 4 249 90 110
Fragments arranged by size:
276 249 110 90 4
EcoO109I rG'GnC_Cy
Cuts at: 0 161 199 486 729
Size: 161 38 287 243
Fragments arranged by size:
287 243 161 38
HaeII r_GCGC'y
Cuts at: 0 40 729
Size: 40 689
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 550 582 729
Size: 256 32 262 32 147
Fragments arranged by size:
262 256 147 32 32
KpnI G_GTAC'C
Cuts at: 0 285 729
Size: 285 444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 729
Size: 296 433
MscI TGG'CCA
Cuts at: 0 195 729
Size: 195 534
MslI CAynn'nnrTG
Cuts at: 0 343 729
Size: 343 386
NcoI C'CATG_G
Cuts at: 0 344 729
Size: 344 385
PfoI T'CCnGG_A
Cuts at: 0 163 613 729
Size: 163 450 116
Fragments arranged by size:
450 163 116
PpuMI rG'GwC_Cy
Cuts at: 0 161 199 486 729
Size: 161 38 287 243
Fragments arranged by size:
287 243 161 38
PshAI GACnn'nnGTC
Cuts at: 0 312 729
Size: 312 417
PstI C_TGCA'G
Cuts at: 0 481 729
Size: 481 248
SacI G_AGCT'C
Cuts at: 0 7 729
Size: 7 722
SanDI GG'GwC_CC
Cuts at: 0 161 729
Size: 161 568
SfcI C'TryA_G
Cuts at: 0 477 729
Size: 477 252
StyI C'CwwG_G
Cuts at: 0 176 344 729
Size: 176 168 385
Fragments arranged by size:
385 176 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 234 729
Size: 234 495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 621 729
Size: 621 108
Enzymes that do cut:
Acc65I AcuI AfeI AloI AvaI BamHI BanI BanII
BbsI BbvCI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaWI BseRI BseYI BsiHKAI Bsp1286I BsrDI BstXI
BstYI BtgI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII
Hin4I KpnI MmeI MscI MslI NcoI PfoI PpuMI
PshAI PstI SacI SanDI SfcI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BpuEI BsaBI BsaHI
BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI