Restriction map for
pFN21AE1736
(Linear) (Six-base) MAPSORT of: KIEE1736.seq Check: 3258 from: 1 to: 726
KIEE1736.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 319 726
Size: 319 407
AatII G_ACGT'C
Cuts at: 0 582 726
Size: 582 144
Acc65I G'GTAC_C
Cuts at: 0 480 726
Size: 480 246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 230 726
Size: 164 66 496
Fragments arranged by size:
496 164 66
AflIII A'CryG_T
Cuts at: 0 215 726
Size: 215 511
ApoI r'AATT_y
Cuts at: 0 200 726
Size: 200 526
AvaI C'yCGr_G
Cuts at: 0 17 726
Size: 17 709
BanI G'GyrC_C
Cuts at: 0 480 726
Size: 480 246
BbsI GAAGACnn'nnnn_
Cuts at: 0 218 726
Size: 218 508
BmrI ACTGGGnnnn_n'
Cuts at: 0 134 697 726
Size: 134 563 29
Fragments arranged by size:
563 134 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 726
Size: 152 574
BsaHI Gr'CG_yC
Cuts at: 0 579 726
Size: 579 147
BsaWI w'CCGG_w
Cuts at: 0 445 726
Size: 445 281
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 541 571 578 608 726
Size: 541 30 7 30 118
Fragments arranged by size:
541 118 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 149 284 338 520 726
Size: 149 135 54 182 206
Fragments arranged by size:
206 182 149 135 54
BseYI C'CCAG_C
Cuts at: 0 116 726
Size: 116 610
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 584 726
Size: 353 231 142
Fragments arranged by size:
353 231 142
BsmI GAATG_Cn'
Cuts at: 0 312 726
Size: 312 414
BspEI T'CCGG_A
Cuts at: 0 445 726
Size: 445 281
BspHI T'CATG_A
Cuts at: 0 302 726
Size: 302 424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 319 726
Size: 319 407
BsrDI GCAATG_nn'
Cuts at: 0 170 545 726
Size: 170 375 181
Fragments arranged by size:
375 181 170
BssHII G'CGCG_C
Cuts at: 0 50 726
Size: 50 676
Bsu36I CC'TnA_GG
Cuts at: 0 503 726
Size: 503 223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 173 726
Size: 173 553
BtsI GCAGTG_nn'
Cuts at: 0 341 726
Size: 341 385
DraI TTT'AAA
Cuts at: 0 399 726
Size: 399 327
EaeI y'GGCC_r
Cuts at: 0 368 726
Size: 368 358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 164 230 726
Size: 152 12 66 496
Fragments arranged by size:
496 152 66 12
EcoRI G'AATT_C
Cuts at: 0 200 726
Size: 200 526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 272 700 726
Size: 272 428 26
Fragments arranged by size:
428 272 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 65 97 726
Size: 65 32 629
Fragments arranged by size:
629 65 32
KpnI G_GTAC'C
Cuts at: 0 484 726
Size: 484 242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 726
Size: 687 39
MscI TGG'CCA
Cuts at: 0 370 726
Size: 370 356
MslI CAynn'nnrTG
Cuts at: 0 158 726
Size: 158 568
NspI r_CATG'y
Cuts at: 0 219 726
Size: 219 507
PciI A'CATG_T
Cuts at: 0 215 726
Size: 215 511
PfoI T'CCnGG_A
Cuts at: 0 37 384 726
Size: 37 347 342
Fragments arranged by size:
347 342 37
ScaI AGT'ACT
Cuts at: 0 569 726
Size: 569 157
TatI w'GTAC_w
Cuts at: 0 289 439 567 726
Size: 289 150 128 159
Fragments arranged by size:
289 159 150 128
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 578 610 726
Size: 578 32 116
Fragments arranged by size:
578 116 32
XmnI GAAnn'nnTTC
Cuts at: 0 200 726
Size: 200 526
ZraI GAC'GTC
Cuts at: 0 580 726
Size: 580 146
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII ApoI AvaI BanI
BbsI BmrI BpmI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsmI BspEI BspHI BspMI BsrDI BssHII Bsu36I
BtgZI BtsI DraI EaeI Eco57MI EcoRI FalI Hin4I
KpnI MmeI MscI MslI NspI PciI PfoI ScaI
TatI TstI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BsrBI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI ClaI CspCI DraIII
DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI