Restriction map for
pFN21AE1735
(Linear) (Six-base) MAPSORT of: KIEE1735.seq Check: 8420 from: 1 to: 726
KIEE1735.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AflIII A'CryG_T
Cuts at: 0 122 726
Size: 122 604
AlwNI CAG_nnn'CTG
Cuts at: 0 357 570 726
Size: 357 213 156
Fragments arranged by size:
357 213 156
ApoI r'AATT_y
Cuts at: 0 199 726
Size: 199 527
AvaI C'yCGr_G
Cuts at: 0 83 221 726
Size: 83 138 505
Fragments arranged by size:
505 138 83
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 700 726
Size: 700 26
BanI G'GyrC_C
Cuts at: 0 46 726
Size: 46 680
BbsI GAAGACnn'nnnn_
Cuts at: 0 107 726
Size: 107 619
BciVI GTATCCnnnnn_n'
Cuts at: 0 514 726
Size: 514 212
BclI T'GATC_A
Cuts at: 0 540 726
Size: 540 186
BglI GCCn_nnn'nGGC
Cuts at: 0 55 726
Size: 55 671
BlpI GC'TnA_GC
Cuts at: 0 365 726
Size: 365 361
Bme1580I G_kGCm'C
Cuts at: 0 51 726
Size: 51 675
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 726
Size: 334 392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 726
Size: 268 458
BsaI GGTCTCn'nnnn_
Cuts at: 0 155 726
Size: 155 571
BsaBI GATnn'nnATC
Cuts at: 0 451 726
Size: 451 275
BsaWI w'CCGG_w
Cuts at: 0 704 726
Size: 704 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 413 726
Size: 40 373 313
Fragments arranged by size:
373 313 40
Bsp1286I G_dGCh'C
Cuts at: 0 51 726
Size: 51 675
BspEI T'CCGG_A
Cuts at: 0 704 726
Size: 704 22
BsrGI T'GTAC_A
Cuts at: 0 377 726
Size: 377 349
BstBI TT'CG_AA
Cuts at: 0 143 726
Size: 143 583
BstEII G'GTnAC_C
Cuts at: 0 624 726
Size: 624 102
BstYI r'GATC_y
Cuts at: 0 228 726
Size: 228 498
BtgI C'CryG_G
Cuts at: 0 10 509 726
Size: 10 499 217
Fragments arranged by size:
499 217 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 510 726
Size: 510 216
BtsI GCAGTG_nn'
Cuts at: 0 167 726
Size: 167 559
ClaI AT'CG_AT
Cuts at: 0 215 726
Size: 215 511
EarI CTCTTCn'nnn_
Cuts at: 0 17 182 328 583 726
Size: 17 165 146 255 143
Fragments arranged by size:
255 165 146 143 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 648 726
Size: 648 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 726
Size: 334 392
EcoO109I rG'GnC_Cy
Cuts at: 0 330 354 411 726
Size: 330 24 57 315
Fragments arranged by size:
330 315 57 24
MspA1I CmG'CkG
Cuts at: 0 12 135 726
Size: 12 123 591
Fragments arranged by size:
591 123 12
NspI r_CATG'y
Cuts at: 0 126 726
Size: 126 600
PciI A'CATG_T
Cuts at: 0 122 726
Size: 122 604
PflMI CCAn_nnn'nTGG
Cuts at: 0 570 726
Size: 570 156
PfoI T'CCnGG_A
Cuts at: 0 182 726
Size: 182 544
PpuMI rG'GwC_Cy
Cuts at: 0 411 726
Size: 411 315
PstI C_TGCA'G
Cuts at: 0 725 726
Size: 725 1
PvuII CAG'CTG
Cuts at: 0 135 726
Size: 135 591
SacII CC_GC'GG
Cuts at: 0 13 726
Size: 13 713
SanDI GG'GwC_CC
Cuts at: 0 411 726
Size: 411 315
SapI GCTCTTCn'nnn_
Cuts at: 0 182 726
Size: 182 544
SfcI C'TryA_G
Cuts at: 0 721 726
Size: 721 5
SmaI CCC'GGG
Cuts at: 0 85 223 726
Size: 85 138 503
Fragments arranged by size:
503 138 85
SmlI C'TyrA_G
Cuts at: 0 283 726
Size: 283 443
StuI AGG'CCT
Cuts at: 0 391 726
Size: 391 335
StyI C'CwwG_G
Cuts at: 0 371 726
Size: 371 355
TatI w'GTAC_w
Cuts at: 0 377 726
Size: 377 349
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 458 490 522 726
Size: 458 32 32 204
Fragments arranged by size:
458 204 32 32
XmaI C'CCGG_G
Cuts at: 0 83 221 726
Size: 83 138 505
Fragments arranged by size:
505 138 83
XmnI GAAnn'nnTTC
Cuts at: 0 322 726
Size: 322 404
Enzymes that do cut:
AflIII AlwNI ApoI AvaI BaeI BanI BbsI BciVI
BclI BglI BlpI Bme1580I BpmI BpuEI BsaI BsaBI
BsaWI BseRI Bsp1286I BspEI BsrGI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI EarI EciI Eco57MI EcoO109I
MspA1I NspI PciI PflMI PfoI PpuMI PstI PvuII
SacII SanDI SapI SfcI SmaI SmlI StuI StyI
TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BamHI BanII BbeI BbvCI BcgI
BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I BsaAI
BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstXI BstZ17I Bsu36I CspCI DraI DraIII DrdI
EaeI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TaqII Tth111I XbaI XcmI XhoI ZraI