Restriction map for
pFN21AE1732
(Linear) (Six-base) MAPSORT of: KIEE1732.seq Check: 3656 from: 1 to: 720
KIEE1732.orf
With 182 enzymes: SgfI *
July 3, 2012 17:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 414 574 720
Size: 414 160 146
Fragments arranged by size:
414 160 146
AgeI A'CCGG_T
Cuts at: 0 214 720
Size: 214 506
AlwNI CAG_nnn'CTG
Cuts at: 0 298 720
Size: 298 422
ApaI G_GGCC'C
Cuts at: 0 59 146 720
Size: 59 87 574
Fragments arranged by size:
574 87 59
AvaI C'yCGr_G
Cuts at: 0 60 720
Size: 60 660
BanII G_rGCy'C
Cuts at: 0 59 146 720
Size: 59 87 574
Fragments arranged by size:
574 87 59
BclI T'GATC_A
Cuts at: 0 608 720
Size: 608 112
BglI GCCn_nnn'nGGC
Cuts at: 0 34 180 720
Size: 34 146 540
Fragments arranged by size:
540 146 34
Bme1580I G_kGCm'C
Cuts at: 0 59 146 720
Size: 59 87 574
Fragments arranged by size:
574 87 59
BmgBI CAC'GTC
Cuts at: 0 192 720
Size: 192 528
BmrI ACTGGGnnnn_n'
Cuts at: 0 319 636 720
Size: 319 317 84
Fragments arranged by size:
319 317 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 631 720
Size: 631 89
BsaWI w'CCGG_w
Cuts at: 0 214 720
Size: 214 506
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 422 452 720
Size: 214 30 178 30 268
Fragments arranged by size:
268 214 178 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 253 616 720
Size: 253 363 104
Fragments arranged by size:
363 253 104
BseYI C'CCAG_C
Cuts at: 0 195 373 550 720
Size: 195 178 177 170
Fragments arranged by size:
195 178 177 170
BsiEI CG_ry'CG
Cuts at: 0 179 720
Size: 179 541
BsiHKAI G_wGCw'C
Cuts at: 0 575 720
Size: 575 145
Bsp1286I G_dGCh'C
Cuts at: 0 59 146 575 720
Size: 59 87 429 145
Fragments arranged by size:
429 145 87 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 414 574 720
Size: 414 160 146
Fragments arranged by size:
414 160 146
BsrBI CCG'CTC
Cuts at: 0 49 720
Size: 49 671
BsrFI r'CCGG_y
Cuts at: 0 214 350 671 720
Size: 214 136 321 49
Fragments arranged by size:
321 214 136 49
Bsu36I CC'TnA_GG
Cuts at: 0 236 418 720
Size: 236 182 302
Fragments arranged by size:
302 236 182
BtgI C'CryG_G
Cuts at: 0 434 720
Size: 434 286
DrdI GACnn_nn'nnGTC
Cuts at: 0 463 720
Size: 463 257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 403 613 720
Size: 403 210 107
Fragments arranged by size:
403 210 107
EcoNI CCTnn'n_nnAGG
Cuts at: 0 421 720
Size: 421 299
EcoO109I rG'GnC_Cy
Cuts at: 0 55 56 142 357 720
Size: 55 1 86 215 363
Fragments arranged by size:
363 215 86 55 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 276 720
Size: 276 444
HaeII r_GCGC'y
Cuts at: 0 381 720
Size: 381 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 628 660 720
Size: 274 32 322 32 60
Fragments arranged by size:
322 274 60 32 32
HincII GTy'rAC
Cuts at: 0 468 720
Size: 468 252
HindIII A'AGCT_T
Cuts at: 0 519 720
Size: 519 201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 720
Size: 101 619
MslI CAynn'nnrTG
Cuts at: 0 632 720
Size: 632 88
MspA1I CmG'CkG
Cuts at: 0 159 207 710 720
Size: 159 48 503 10
Fragments arranged by size:
503 159 48 10
PasI CC'CwG_GG
Cuts at: 0 139 720
Size: 139 581
PflMI CCAn_nnn'nTGG
Cuts at: 0 72 720
Size: 72 648
PpuMI rG'GwC_Cy
Cuts at: 0 357 720
Size: 357 363
PspOMI G'GGCC_C
Cuts at: 0 55 142 720
Size: 55 87 578
Fragments arranged by size:
578 87 55
PstI C_TGCA'G
Cuts at: 0 164 206 720
Size: 164 42 514
Fragments arranged by size:
514 164 42
PvuI CG_AT'CG
Cuts at: 0 179 720
Size: 179 541
SfcI C'TryA_G
Cuts at: 0 160 202 705 720
Size: 160 42 503 15
Fragments arranged by size:
503 160 42 15
SgrAI Cr'CCGG_yG
Cuts at: 0 214 720
Size: 214 506
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 201 233 422 454 720
Size: 201 32 189 32 266
Fragments arranged by size:
266 201 189 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 42 262 642 720
Size: 42 220 380 78
Fragments arranged by size:
380 220 78 42
Enzymes that do cut:
AarI AgeI AlwNI ApaI AvaI BanII BclI BglI
Bme1580I BmgBI BmrI BsaI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI Bsu36I BtgI
DrdI EciI EcoNI EcoO109I FalI HaeII Hin4I HincII
HindIII MmeI MslI MspA1I PasI PflMI PpuMI PspOMI
PstI PvuI SfcI SgrAI TstI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglII BlpI BmtI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsgI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoRI EcoRV FseI FspI FspAI HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
XbaI XcmI XhoI XmaI XmnI ZraI