Restriction map for
pFN21AE1730
(Linear) (Six-base) MAPSORT of: KIEE1730.seq Check: 8984 from: 1 to: 714
KIEE1730.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
Acc65I G'GTAC_C
Cuts at: 0 492 714
Size: 492 222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 577 714
Size: 376 201 137
Fragments arranged by size:
376 201 137
AlwNI CAG_nnn'CTG
Cuts at: 0 651 714
Size: 651 63
ApaLI G'TGCA_C
Cuts at: 0 630 714
Size: 630 84
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 476 509 714
Size: 476 33 205
Fragments arranged by size:
476 205 33
BanI G'GyrC_C
Cuts at: 0 181 220 492 547 561 714
Size: 181 39 272 55 14 153
Fragments arranged by size:
272 181 153 55 39 14
BbeI G_GCGC'C
Cuts at: 0 185 565 714
Size: 185 380 149
Fragments arranged by size:
380 185 149
Bme1580I G_kGCm'C
Cuts at: 0 634 714
Size: 634 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 307 432 714
Size: 1 306 125 282
Fragments arranged by size:
306 282 125 1
BsaHI Gr'CG_yC
Cuts at: 0 182 446 562 714
Size: 182 264 116 152
Fragments arranged by size:
264 182 152 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 223 253 714
Size: 223 30 461
Fragments arranged by size:
461 223 30
BseYI C'CCAG_C
Cuts at: 0 63 81 344 714
Size: 63 18 263 370
Fragments arranged by size:
370 263 63 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 664 714
Size: 520 144 50
Fragments arranged by size:
520 144 50
BsiHKAI G_wGCw'C
Cuts at: 0 634 714
Size: 634 80
BsmBI CGTCTCn'nnnn_
Cuts at: 0 453 568 714
Size: 453 115 146
Fragments arranged by size:
453 146 115
Bsp1286I G_dGCh'C
Cuts at: 0 634 714
Size: 634 80
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 59 173 611 714
Size: 59 114 438 103
Fragments arranged by size:
438 114 103 59
BsrFI r'CCGG_y
Cuts at: 0 178 714
Size: 178 536
BsrGI T'GTAC_A
Cuts at: 0 476 714
Size: 476 238
BssHII G'CGCG_C
Cuts at: 0 4 6 714
Size: 4 2 708
Fragments arranged by size:
708 4 2
BstYI r'GATC_y
Cuts at: 0 647 714
Size: 647 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 282 714
Size: 282 432
DraIII CAC_nnn'GTG
Cuts at: 0 554 714
Size: 554 160
EaeI y'GGCC_r
Cuts at: 0 101 194 714
Size: 101 93 520
Fragments arranged by size:
520 101 93
EarI CTCTTCn'nnn_
Cuts at: 0 61 141 313 714
Size: 61 80 172 401
Fragments arranged by size:
401 172 80 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 307 376 432 577 714
Size: 1 306 69 56 145 137
Fragments arranged by size:
306 145 137 69 56 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 286 714
Size: 286 428
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 381 714
Size: 381 333
HaeII r_GCGC'y
Cuts at: 0 185 565 640 714
Size: 185 380 75 74
Fragments arranged by size:
380 185 75 74
KasI G'GCGC_C
Cuts at: 0 181 561 714
Size: 181 380 153
Fragments arranged by size:
380 181 153
KpnI G_GTAC'C
Cuts at: 0 496 714
Size: 496 218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 714
Size: 476 238
MscI TGG'CCA
Cuts at: 0 196 714
Size: 196 518
MslI CAynn'nnrTG
Cuts at: 0 673 714
Size: 673 41
MspA1I CmG'CkG
Cuts at: 0 437 714
Size: 437 277
NarI GG'CG_CC
Cuts at: 0 182 562 714
Size: 182 380 152
Fragments arranged by size:
380 182 152
PflMI CCAn_nnn'nTGG
Cuts at: 0 410 714
Size: 410 304
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 22 54 714
Size: 22 32 660
Fragments arranged by size:
660 32 22
SapI GCTCTTCn'nnn_
Cuts at: 0 61 714
Size: 61 653
SexAI A'CCwGG_T
Cuts at: 0 550 714
Size: 550 164
SfoI GGC'GCC
Cuts at: 0 183 563 714
Size: 183 380 151
Fragments arranged by size:
380 183 151
SgrAI Cr'CCGG_yG
Cuts at: 0 178 714
Size: 178 536
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 208 442 714
Size: 73 135 234 272
Fragments arranged by size:
272 234 135 73
TatI w'GTAC_w
Cuts at: 0 476 677 714
Size: 476 201 37
Fragments arranged by size:
476 201 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 223 255 714
Size: 223 32 459
Fragments arranged by size:
459 223 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 204 714
Size: 204 510
Enzymes that do cut:
Acc65I AcuI AlwNI ApaLI BaeI BanI BbeI Bme1580I
BpmI BsaHI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrFI BsrGI BssHII BstYI BtgZI DraIII EaeI
EarI Eco57MI EcoNI FalI HaeII KasI KpnI MmeI
MscI MslI MspA1I NarI PflMI PsrI SapI SexAI
SfoI SgrAI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BamHI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BseRI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoO109I EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI MfeI MluI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SfcI SfiI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI