(Linear) (Six-base) MAPSORT of: KIEE1729.seq  Check: 4880  from: 1  to: 711



KIEE1729.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:46  ..

Acc65I G'GTAC_C

Cuts at:      0     103     711 
   Size:        103     608
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     358     390     711 
   Size:        358      32     321

  Fragments arranged by size: 

                 358     321      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      67     415     679     711 
   Size:         67     348     264      32

  Fragments arranged by size: 

                 348     264      67      32
 
ApoI r'AATT_y

Cuts at:      0     253     331     711 
   Size:        253      78     380

  Fragments arranged by size: 

                 380     253      78
 
BanI G'GyrC_C

Cuts at:      0     103     711 
   Size:        103     608
 
BanII G_rGCy'C

Cuts at:      0      47     711 
   Size:         47     664
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     305     711 
   Size:        305     406
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     654     711 
   Size:        654      57
 
BclI T'GATC_A

Cuts at:      0     705     711 
   Size:        705       6
 
BfrBI ATG'CAT

Cuts at:      0     193     711 
   Size:        193     518
 
BglI GCCn_nnn'nGGC

Cuts at:      0      30     711 
   Size:         30     681
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     711 
   Size:        574     137
 
BsaBI GATnn'nnATC

Cuts at:      0     704     711 
   Size:        704       7
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     358     388     711 
   Size:        358      30     323

  Fragments arranged by size: 

                 358     323      30
 
BseYI C'CCAG_C

Cuts at:      0     607     711 
   Size:        607     104
 
BsmI GAATG_Cn'

Cuts at:      0     252     397     575     711 
   Size:        252     145     178     136

  Fragments arranged by size: 

                 252     178     145     136
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     711 
   Size:         47     664
 
BsrDI GCAATG_nn'

Cuts at:      0     377     389     711 
   Size:        377      12     322

  Fragments arranged by size: 

                 377     322      12
 
BstYI r'GATC_y

Cuts at:      0     411     711 
   Size:        411     300
 
Bsu36I CC'TnA_GG

Cuts at:      0     416     711 
   Size:        416     295
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      99     711 
   Size:         99     612
 
BtsI GCAGTG_nn'

Cuts at:      0     377     711 
   Size:        377     334
 
EarI CTCTTCn'nnn_

Cuts at:      0     287     711 
   Size:        287     424
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     711 
   Size:        574     137
 
EcoO109I rG'GnC_Cy

Cuts at:      0      90     711 
   Size:         90     621
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     589     711 
   Size:        589     122
 
KpnI G_GTAC'C

Cuts at:      0     107     711 
   Size:        107     604
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     711 
   Size:        344     367
 
MslI CAynn'nnrTG

Cuts at:      0     233     382     711 
   Size:        233     149     329

  Fragments arranged by size: 

                 329     233     149
 
MspA1I CmG'CkG

Cuts at:      0      37     711 
   Size:         37     674
 
NsiI A_TGCA'T

Cuts at:      0     195     711 
   Size:        195     516
 
NspI r_CATG'y

Cuts at:      0     193     711 
   Size:        193     518
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     358     390     711 
   Size:        358      32     321

  Fragments arranged by size: 

                 358     321      32
 
StuI AGG'CCT

Cuts at:      0     400     711 
   Size:        400     311
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     221     711 
   Size:        221     490
 
XmnI GAAnn'nnTTC

Cuts at:      0     573     711 
   Size:        573     138
 

 Enzymes that do cut:

   Acc65I     AloI    AlwNI     ApoI     BanI    BanII     BbsI    BciVI
     BclI    BfrBI     BglI     BpmI    BsaBI    BsaXI    BseYI     BsmI
 Bsp1286I    BsrDI    BstYI   Bsu36I    BtgZI     BtsI     EarI  Eco57MI
 EcoO109I     FalI     KpnI     MmeI     MslI   MspA1I     NsiI     NspI
     PpiI     StuI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
     BtgI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI