Restriction map for
pFN21AE1728
(Linear) (Six-base) MAPSORT of: KIEE1728.seq Check: 5288 from: 1 to: 708
KIEE1728.orf
With 182 enzymes: SgfI *
July 3, 2012 17:00 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 409 708
Size: 409 299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 120 708
Size: 120 588
AlwNI CAG_nnn'CTG
Cuts at: 0 55 708
Size: 55 653
AvaI C'yCGr_G
Cuts at: 0 18 708
Size: 18 690
AvrII C'CTAG_G
Cuts at: 0 462 708
Size: 462 246
BamHI G'GATC_C
Cuts at: 0 144 250 479 708
Size: 144 106 229 229
Fragments arranged by size:
229 229 144 106
BanII G_rGCy'C
Cuts at: 0 656 708
Size: 656 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 228 262 708
Size: 228 34 446
Fragments arranged by size:
446 228 34
BglI GCCn_nnn'nGGC
Cuts at: 0 593 708
Size: 593 115
BglII A'GATC_T
Cuts at: 0 7 668 708
Size: 7 661 40
Fragments arranged by size:
661 40 7
BlpI GC'TnA_GC
Cuts at: 0 235 337 708
Size: 235 102 371
Fragments arranged by size:
371 235 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 415 451 549 708
Size: 415 36 98 159
Fragments arranged by size:
415 159 98 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 708
Size: 411 297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 708
Size: 469 239
BsaAI yAC'GTr
Cuts at: 0 132 708
Size: 132 576
BsaHI Gr'CG_yC
Cuts at: 0 410 708
Size: 410 298
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 14 44 708
Size: 14 30 664
Fragments arranged by size:
664 30 14
BseYI C'CCAG_C
Cuts at: 0 123 148 566 708
Size: 123 25 418 142
Fragments arranged by size:
418 142 123 25
BsiHKAI G_wGCw'C
Cuts at: 0 419 425 708
Size: 419 6 283
Fragments arranged by size:
419 283 6
Bsp1286I G_dGCh'C
Cuts at: 0 419 425 656 708
Size: 419 6 231 52
Fragments arranged by size:
419 231 52 6
BsrBI CCG'CTC
Cuts at: 0 189 708
Size: 189 519
BsrFI r'CCGG_y
Cuts at: 0 674 708
Size: 674 34
BssSI C'ACGA_G
Cuts at: 0 418 708
Size: 418 290
BstAPI GCAn_nnn'nTGC
Cuts at: 0 333 708
Size: 333 375
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 561 708
Size: 156 405 147
Fragments arranged by size:
405 156 147
BstYI r'GATC_y
Cuts at: 0 7 144 250 479 668 708
Size: 7 137 106 229 189 40
Fragments arranged by size:
229 189 137 106 40 7
BtgI C'CryG_G
Cuts at: 0 371 590 708
Size: 371 219 118
Fragments arranged by size:
371 219 118
BtsI GCAGTG_nn'
Cuts at: 0 315 708
Size: 315 393
EarI CTCTTCn'nnn_
Cuts at: 0 644 708
Size: 644 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 708
Size: 411 297
EcoNI CCTnn'n_nnAGG
Cuts at: 0 487 708
Size: 487 221
EcoO109I rG'GnC_Cy
Cuts at: 0 52 242 692 708
Size: 52 190 450 16
Fragments arranged by size:
450 190 52 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 395 427 708
Size: 395 32 281
Fragments arranged by size:
395 281 32
MspA1I CmG'CkG
Cuts at: 0 373 664 708
Size: 373 291 44
Fragments arranged by size:
373 291 44
PflMI CCAn_nnn'nTGG
Cuts at: 0 539 708
Size: 539 169
PfoI T'CCnGG_A
Cuts at: 0 521 708
Size: 521 187
PpuMI rG'GwC_Cy
Cuts at: 0 692 708
Size: 692 16
SacII CC_GC'GG
Cuts at: 0 374 708
Size: 374 334
SanDI GG'GwC_CC
Cuts at: 0 692 708
Size: 692 16
SapI GCTCTTCn'nnn_
Cuts at: 0 644 708
Size: 644 64
SfcI C'TryA_G
Cuts at: 0 378 708
Size: 378 330
SmaI CCC'GGG
Cuts at: 0 20 708
Size: 20 688
SmlI C'TyrA_G
Cuts at: 0 484 708
Size: 484 224
SspI AAT'ATT
Cuts at: 0 81 708
Size: 81 627
StuI AGG'CCT
Cuts at: 0 71 708
Size: 71 637
StyI C'CwwG_G
Cuts at: 0 462 708
Size: 462 246
TatI w'GTAC_w
Cuts at: 0 536 708
Size: 536 172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 168 200 708
Size: 168 32 508
Fragments arranged by size:
508 168 32
XmaI C'CCGG_G
Cuts at: 0 18 708
Size: 18 690
Enzymes that do cut:
AhdI AlfI AlwNI AvaI AvrII BamHI BanII BcgI
BglI BglII BlpI BmrI BpmI BpuEI BsaAI BsaHI
BsaXI BseYI BsiHKAI Bsp1286I BsrBI BsrFI BssSI BstAPI
BstXI BstYI BtgI BtsI EarI Eco57MI EcoNI EcoO109I
Hin4I MspA1I PflMI PfoI PpuMI SacII SanDI SapI
SfcI SmaI SmlI SspI StuI StyI TatI TstI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI BaeI BanI BbeI BbsI BbvCI BciVI
BclI BfrBI Bme1580I BmgBI BmtI BplI Bpu10I BsaI
BsaBI BsaWI BseRI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SwaI TaqII Tth111I XbaI XcmI
XhoI XmnI ZraI