(Linear) (Six-base) MAPSORT of: KIEE1726.seq  Check: 5623  from: 1  to: 708



KIEE1726.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:46  ..

AgeI A'CCGG_T

Cuts at:      0     512     708 
   Size:        512     196
 
AseI AT'TA_AT

Cuts at:      0     601     708 
   Size:        601     107
 
BamHI G'GATC_C

Cuts at:      0     175     708 
   Size:        175     533
 
BanII G_rGCy'C

Cuts at:      0     299     708 
   Size:        299     409
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     374     708 
   Size:        374     334
 
BglI GCCn_nnn'nGGC

Cuts at:      0      17     708 
   Size:         17     691
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     304     708 
   Size:        304     404
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     500     708 
   Size:        500     208
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467     708 
   Size:        467     241
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     339     708 
   Size:        339     369
 
BsaAI yAC'GTr

Cuts at:      0      51     708 
   Size:         51     657
 
BsaHI Gr'CG_yC

Cuts at:      0     417     708 
   Size:        417     291
 
BsaWI w'CCGG_w

Cuts at:      0     512     708 
   Size:        512     196
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     630     646     708 
   Size:        630      16      62

  Fragments arranged by size: 

                 630      62      16
 
Bsp1286I G_dGCh'C

Cuts at:      0     299     708 
   Size:        299     409
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     384     708 
   Size:        384     324
 
BsrBI CCG'CTC

Cuts at:      0     542     708 
   Size:        542     166
 
BsrFI r'CCGG_y

Cuts at:      0     121     512     708 
   Size:        121     391     196

  Fragments arranged by size: 

                 391     196     121
 
BstYI r'GATC_y

Cuts at:      0     175     708 
   Size:        175     533
 
BtgI C'CryG_G

Cuts at:      0     375     708 
   Size:        375     333
 
BtsI GCAGTG_nn'

Cuts at:      0     424     708 
   Size:        424     284
 
EaeI y'GGCC_r

Cuts at:      0      18     378     708 
   Size:         18     360     330

  Fragments arranged by size: 

                 360     330      18
 
EarI CTCTTCn'nnn_

Cuts at:      0     469     641     708 
   Size:        469     172      67

  Fragments arranged by size: 

                 469     172      67
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      55     140     708 
   Size:         55      85     568

  Fragments arranged by size: 

                 568      85      55
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467     708 
   Size:        467     241
 
EcoO109I rG'GnC_Cy

Cuts at:      0     226     257     708 
   Size:        226      31     451

  Fragments arranged by size: 

                 451     226      31
 
HaeII r_GCGC'y

Cuts at:      0     138     708 
   Size:        138     570
 
HincII GTy'rAC

Cuts at:      0     555     708 
   Size:        555     153
 
MscI TGG'CCA

Cuts at:      0      20     380     708 
   Size:         20     360     328

  Fragments arranged by size: 

                 360     328      20
 
MspA1I CmG'CkG

Cuts at:      0      16     667     708 
   Size:         16     651      41

  Fragments arranged by size: 

                 651      41      16
 
NaeI GCC'GGC

Cuts at:      0     123     708 
   Size:        123     585
 
NgoMIV G'CCGG_C

Cuts at:      0     121     708 
   Size:        121     587
 
PpuMI rG'GwC_Cy

Cuts at:      0     257     708 
   Size:        257     451
 
PshAI GACnn'nnGTC

Cuts at:      0     568     708 
   Size:        568     140
 
PstI C_TGCA'G

Cuts at:      0     113     434     660     708 
   Size:        113     321     226      48

  Fragments arranged by size: 

                 321     226     113      48
 
PvuII CAG'CTG

Cuts at:      0     667     708 
   Size:        667      41
 
SfcI C'TryA_G

Cuts at:      0     109     430     656     708 
   Size:        109     321     226      52

  Fragments arranged by size: 

                 321     226     109      52
 
SgrAI Cr'CCGG_yG

Cuts at:      0     512     708 
   Size:        512     196
 

 Enzymes that do cut:

     AgeI     AseI    BamHI    BanII     BbsI     BglI     BmrI     BplI
     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BseRI Bsp1286I    BspMI
    BsrBI    BsrFI    BstYI     BtgI     BtsI     EaeI     EarI     EciI
  Eco57MI EcoO109I    HaeII   HincII     MscI   MspA1I     NaeI   NgoMIV
    PpuMI    PshAI     PstI    PvuII     SfcI    SgrAI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmtI   Bpu10I    BpuEI    BsaBI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI