Restriction map for
pFN21AE1724
(Linear) (Six-base) MAPSORT of: KIEE1724.seq Check: 8082 from: 1 to: 702
KIEE1724.orf
With 182 enzymes: SgfI *
July 15, 2010 18:46 ..
AccI GT'mk_AC
Cuts at: 0 557 702
Size: 557 145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 702
Size: 493 209
AflIII A'CryG_T
Cuts at: 0 552 702
Size: 552 150
AlwNI CAG_nnn'CTG
Cuts at: 0 516 702
Size: 516 186
BanII G_rGCy'C
Cuts at: 0 76 279 702
Size: 76 203 423
Fragments arranged by size:
423 203 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 144 257 702
Size: 144 113 445
Fragments arranged by size:
445 144 113
BciVI GTATCCnnnnn_n'
Cuts at: 0 274 618 702
Size: 274 344 84
Fragments arranged by size:
344 274 84
BfrBI ATG'CAT
Cuts at: 0 60 702
Size: 60 642
BglI GCCn_nnn'nGGC
Cuts at: 0 136 702
Size: 136 566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 702
Size: 65 637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 702
Size: 682 20
BsaAI yAC'GTr
Cuts at: 0 555 702
Size: 555 147
BsaBI GATnn'nnATC
Cuts at: 0 386 702
Size: 386 316
BsmI GAATG_Cn'
Cuts at: 0 356 545 702
Size: 356 189 157
Fragments arranged by size:
356 189 157
Bsp1286I G_dGCh'C
Cuts at: 0 76 279 702
Size: 76 203 423
Fragments arranged by size:
423 203 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 585 599 702
Size: 585 14 103
Fragments arranged by size:
585 103 14
BsrFI r'CCGG_y
Cuts at: 0 66 702
Size: 66 636
BsrGI T'GTAC_A
Cuts at: 0 444 702
Size: 444 258
BssSI C'ACGA_G
Cuts at: 0 99 702
Size: 99 603
BstXI CCAn_nnnn'nTGG
Cuts at: 0 90 171 406 702
Size: 90 81 235 296
Fragments arranged by size:
296 235 90 81
BstZ17I GTA'TAC
Cuts at: 0 558 702
Size: 558 144
BtgI C'CryG_G
Cuts at: 0 77 363 702
Size: 77 286 339
Fragments arranged by size:
339 286 77
EaeI y'GGCC_r
Cuts at: 0 80 161 702
Size: 80 81 541
Fragments arranged by size:
541 81 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 493 702
Size: 65 428 209
Fragments arranged by size:
428 209 65
EcoO109I rG'GnC_Cy
Cuts at: 0 513 702
Size: 513 189
EcoRV GAT'ATC
Cuts at: 0 376 702
Size: 376 326
HincII GTy'rAC
Cuts at: 0 600 702
Size: 600 102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 702
Size: 458 244
MscI TGG'CCA
Cuts at: 0 82 163 702
Size: 82 81 539
Fragments arranged by size:
539 82 81
MslI CAynn'nnrTG
Cuts at: 0 55 61 169 702
Size: 55 6 108 533
Fragments arranged by size:
533 108 55 6
NaeI GCC'GGC
Cuts at: 0 68 702
Size: 68 634
NcoI C'CATG_G
Cuts at: 0 363 702
Size: 363 339
NgoMIV G'CCGG_C
Cuts at: 0 66 702
Size: 66 636
NsiI A_TGCA'T
Cuts at: 0 62 702
Size: 62 640
NspI r_CATG'y
Cuts at: 0 64 702
Size: 64 638
PasI CC'CwG_GG
Cuts at: 0 7 702
Size: 7 695
PfoI T'CCnGG_A
Cuts at: 0 42 702
Size: 42 660
PstI C_TGCA'G
Cuts at: 0 17 437 596 702
Size: 17 420 159 106
Fragments arranged by size:
420 159 106 17
SbfI CC_TGCA'GG
Cuts at: 0 596 702
Size: 596 106
SfcI C'TryA_G
Cuts at: 0 13 433 592 702
Size: 13 420 159 110
Fragments arranged by size:
420 159 110 13
SmlI C'TyrA_G
Cuts at: 0 661 702
Size: 661 41
StyI C'CwwG_G
Cuts at: 0 363 702
Size: 363 339
TatI w'GTAC_w
Cuts at: 0 413 444 635 702
Size: 413 31 191 67
Fragments arranged by size:
413 191 67 31
Tth111I GACn'n_nGTC
Cuts at: 0 220 702
Size: 220 482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 132 702
Size: 132 570
Enzymes that do cut:
AccI AcuI AflIII AlwNI BanII BbsI BciVI BfrBI
BglI BpmI BpuEI BsaAI BsaBI BsmI Bsp1286I BspMI
BsrFI BsrGI BssSI BstXI BstZ17I BtgI EaeI Eco57MI
EcoO109I EcoRV HincII MmeI MscI MslI NaeI NcoI
NgoMIV NsiI NspI PasI PfoI PstI SbfI SfcI
SmlI StyI TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BclI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BssHII BstAPI BstBI BstEII BstYI Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI
MspA1I NarI NdeI NheI NotI NruI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI XbaI XhoI XmaI XmnI ZraI