Restriction map for
pFN21AE1715
(Linear) (Six-base) MAPSORT of: KIEE1715.seq Check: 2323 from: 1 to: 654
KIEE1715.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 398 654
Size: 398 256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 654
Size: 337 317
AfeI AGC'GCT
Cuts at: 0 214 310 654
Size: 214 96 344
Fragments arranged by size:
344 214 96
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 427 459 654
Size: 427 32 195
Fragments arranged by size:
427 195 32
AlwNI CAG_nnn'CTG
Cuts at: 0 651 654
Size: 651 3
AvaI C'yCGr_G
Cuts at: 0 8 570 654
Size: 8 562 84
Fragments arranged by size:
562 84 8
BanI G'GyrC_C
Cuts at: 0 256 654
Size: 256 398
BbeI G_GCGC'C
Cuts at: 0 260 654
Size: 260 394
BbvCI CC'TCA_GC
Cuts at: 0 459 654
Size: 459 195
BglII A'GATC_T
Cuts at: 0 188 512 654
Size: 188 324 142
Fragments arranged by size:
324 188 142
Bme1580I G_kGCm'C
Cuts at: 0 404 654
Size: 404 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 654
Size: 133 521
Bpu10I CC'TnA_GC
Cuts at: 0 459 654
Size: 459 195
BsaI GGTCTCn'nnnn_
Cuts at: 0 448 654
Size: 448 206
BsaHI Gr'CG_yC
Cuts at: 0 257 654
Size: 257 397
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 654
Size: 269 385
BsiHKAI G_wGCw'C
Cuts at: 0 47 127 654
Size: 47 80 527
Fragments arranged by size:
527 80 47
BsmI GAATG_Cn'
Cuts at: 0 490 654
Size: 490 164
Bsp1286I G_dGCh'C
Cuts at: 0 47 127 404 654
Size: 47 80 277 250
Fragments arranged by size:
277 250 80 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 86 389 398 654
Size: 86 303 9 256
Fragments arranged by size:
303 256 86 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 135 654
Size: 135 519
BstYI r'GATC_y
Cuts at: 0 188 512 654
Size: 188 324 142
Fragments arranged by size:
324 188 142
BtgI C'CryG_G
Cuts at: 0 243 608 654
Size: 243 365 46
Fragments arranged by size:
365 243 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 261 654
Size: 261 393
BtsI GCAGTG_nn'
Cuts at: 0 257 643 654
Size: 257 386 11
Fragments arranged by size:
386 257 11
EaeI y'GGCC_r
Cuts at: 0 204 654
Size: 204 450
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 337 654
Size: 133 204 317
Fragments arranged by size:
317 204 133
EcoO109I rG'GnC_Cy
Cuts at: 0 5 654
Size: 5 649
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 456 654
Size: 456 198
HaeII r_GCGC'y
Cuts at: 0 216 260 312 654
Size: 216 44 52 342
Fragments arranged by size:
342 216 52 44
KasI G'GCGC_C
Cuts at: 0 256 654
Size: 256 398
MscI TGG'CCA
Cuts at: 0 206 654
Size: 206 448
MslI CAynn'nnrTG
Cuts at: 0 133 160 361 595 654
Size: 133 27 201 234 59
Fragments arranged by size:
234 201 133 59 27
MspA1I CmG'CkG
Cuts at: 0 202 654
Size: 202 452
NarI GG'CG_CC
Cuts at: 0 257 654
Size: 257 397
PpuMI rG'GwC_Cy
Cuts at: 0 5 654
Size: 5 649
PstI C_TGCA'G
Cuts at: 0 638 654
Size: 638 16
SanDI GG'GwC_CC
Cuts at: 0 5 654
Size: 5 649
SfcI C'TryA_G
Cuts at: 0 634 654
Size: 634 20
SfoI GGC'GCC
Cuts at: 0 258 654
Size: 258 396
SmaI CCC'GGG
Cuts at: 0 10 572 654
Size: 10 562 82
Fragments arranged by size:
562 82 10
StyI C'CwwG_G
Cuts at: 0 464 654
Size: 464 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 550 582 654
Size: 550 32 72
Fragments arranged by size:
550 72 32
XmaI C'CCGG_G
Cuts at: 0 8 570 654
Size: 8 562 84
Fragments arranged by size:
562 84 8
Enzymes that do cut:
AarI AcuI AfeI AloI AlwNI AvaI BanI BbeI
BbvCI BglII Bme1580I BpmI Bpu10I BsaI BsaHI BsgI
BsiHKAI BsmI Bsp1286I BspMI BstXI BstYI BtgI BtgZI
BtsI EaeI Eco57MI EcoO109I FalI HaeII KasI MscI
MslI MspA1I NarI PpuMI PstI SanDI SfcI SfoI
SmaI StyI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbsI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmnI ZraI