Restriction map for
pFN21AE1714
(Linear) (Six-base) MAPSORT of: KIEE1714.seq Check: 525 from: 1 to: 654
KIEE1714.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 562 654
Size: 388 174 92
Fragments arranged by size:
388 174 92
AlwNI CAG_nnn'CTG
Cuts at: 0 138 654
Size: 138 516
ApoI r'AATT_y
Cuts at: 0 148 355 654
Size: 148 207 299
Fragments arranged by size:
299 207 148
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1 34 654
Size: 1 33 620
Fragments arranged by size:
620 33 1
BanII G_rGCy'C
Cuts at: 0 201 515 654
Size: 201 314 139
Fragments arranged by size:
314 201 139
BglII A'GATC_T
Cuts at: 0 578 654
Size: 578 76
BmrI ACTGGGnnnn_n'
Cuts at: 0 22 30 654
Size: 22 8 624
Fragments arranged by size:
624 22 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 597 654
Size: 597 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 654
Size: 60 594
BsaHI Gr'CG_yC
Cuts at: 0 527 654
Size: 527 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 654
Size: 538 30 86
Fragments arranged by size:
538 86 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 654
Size: 289 365
BseYI C'CCAG_C
Cuts at: 0 156 330 654
Size: 156 174 324
Fragments arranged by size:
324 174 156
Bsp1286I G_dGCh'C
Cuts at: 0 201 515 654
Size: 201 314 139
Fragments arranged by size:
314 201 139
BsrDI GCAATG_nn'
Cuts at: 0 236 654
Size: 236 418
BstAPI GCAn_nnn'nTGC
Cuts at: 0 341 654
Size: 341 313
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 522 654
Size: 12 510 132
Fragments arranged by size:
510 132 12
BstYI r'GATC_y
Cuts at: 0 578 654
Size: 578 76
BtgI C'CryG_G
Cuts at: 0 30 311 436 654
Size: 30 281 125 218
Fragments arranged by size:
281 218 125 30
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 240 275 654
Size: 240 35 379
Fragments arranged by size:
379 240 35
EaeI y'GGCC_r
Cuts at: 0 433 654
Size: 433 221
EarI CTCTTCn'nnn_
Cuts at: 0 266 654
Size: 266 388
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 654
Size: 40 614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 562 654
Size: 388 174 92
Fragments arranged by size:
388 174 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 654
Size: 538 32 84
Fragments arranged by size:
538 84 32
MspA1I CmG'CkG
Cuts at: 0 32 330 654
Size: 32 298 324
Fragments arranged by size:
324 298 32
NcoI C'CATG_G
Cuts at: 0 311 436 654
Size: 311 125 218
Fragments arranged by size:
311 218 125
NdeI CA'TA_TG
Cuts at: 0 323 654
Size: 323 331
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 654
Size: 312 342
PfoI T'CCnGG_A
Cuts at: 0 59 654
Size: 59 595
PvuII CAG'CTG
Cuts at: 0 330 654
Size: 330 324
SacII CC_GC'GG
Cuts at: 0 33 654
Size: 33 621
ScaI AGT'ACT
Cuts at: 0 379 654
Size: 379 275
SmlI C'TyrA_G
Cuts at: 0 75 654
Size: 75 579
StyI C'CwwG_G
Cuts at: 0 311 436 654
Size: 311 125 218
Fragments arranged by size:
311 218 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 654
Size: 226 428
TatI w'GTAC_w
Cuts at: 0 95 377 654
Size: 95 282 277
Fragments arranged by size:
282 277 95
XmnI GAAnn'nnTTC
Cuts at: 0 547 654
Size: 547 107
Enzymes that do cut:
AcuI AlwNI ApoI BaeI BanII BglII BmrI BplI
BpuEI BsaHI BsaXI BseRI BseYI Bsp1286I BsrDI BstAPI
BstXI BstYI BtgI CspCI EaeI EarI EciI Eco57MI
Hin4I MspA1I NcoI NdeI PflMI PfoI PvuII SacII
ScaI SmlI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmtI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SapI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI