(Linear) (Six-base) MAPSORT of: KIEE1712.seq  Check: 3657  from: 1  to: 648



KIEE1712.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:45  ..

AccI GT'mk_AC

Cuts at:      0     410     648 
   Size:        410     238
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     648 
   Size:         94     554
 
ApaLI G'TGCA_C

Cuts at:      0     566     648 
   Size:        566      82
 
ApoI r'AATT_y

Cuts at:      0      49     263     648 
   Size:         49     214     385

  Fragments arranged by size: 

                 385     214      49
 
AvaI C'yCGr_G

Cuts at:      0     579     648 
   Size:        579      69
 
BfrBI ATG'CAT

Cuts at:      0     148     648 
   Size:        148     500
 
Bme1580I G_kGCm'C

Cuts at:      0     570     648 
   Size:        570      78
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      29     648 
   Size:         29     619
 
BseYI C'CCAG_C

Cuts at:      0      35     648 
   Size:         35     613
 
BsiHKAI G_wGCw'C

Cuts at:      0     570     648 
   Size:        570      78
 
Bsp1286I G_dGCh'C

Cuts at:      0     570     648 
   Size:        570      78
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     574     648 
   Size:        574      74
 
BstYI r'GATC_y

Cuts at:      0      69     490     648 
   Size:         69     421     158

  Fragments arranged by size: 

                 421     158      69
 
BstZ17I GTA'TAC

Cuts at:      0     411     648 
   Size:        411     237
 
BtgI C'CryG_G

Cuts at:      0     185     648 
   Size:        185     463
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     159     648 
   Size:        159     489
 
BtsI GCAGTG_nn'

Cuts at:      0     505     648 
   Size:        505     143
 
DraI TTT'AAA

Cuts at:      0     588     648 
   Size:        588      60
 
EarI CTCTTCn'nnn_

Cuts at:      0      73     648 
   Size:         73     575
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     648 
   Size:         94     554
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      32     648 
   Size:         32     616
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     505     648 
   Size:        505     143
 
MslI CAynn'nnrTG

Cuts at:      0     205     631     648 
   Size:        205     426      17

  Fragments arranged by size: 

                 426     205      17
 
MspA1I CmG'CkG

Cuts at:      0      35     648 
   Size:         35     613
 
NcoI C'CATG_G

Cuts at:      0     185     648 
   Size:        185     463
 
NsiI A_TGCA'T

Cuts at:      0     150     648 
   Size:        150     498
 
NspI r_CATG'y

Cuts at:      0     636     648 
   Size:        636      12
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     487     648 
   Size:        487     161
 
PsiI TTA'TAA

Cuts at:      0      62     648 
   Size:         62     586
 
PspXI vC'TCGA_Gb

Cuts at:      0     579     648 
   Size:        579      69
 
PvuII CAG'CTG

Cuts at:      0      35     648 
   Size:         35     613
 
SfcI C'TryA_G

Cuts at:      0     179     648 
   Size:        179     469
 
SmlI C'TyrA_G

Cuts at:      0     579     648 
   Size:        579      69
 
SpeI A'CTAG_T

Cuts at:      0     468     648 
   Size:        468     180
 
SphI G_CATG'C

Cuts at:      0     636     648 
   Size:        636      12
 
StyI C'CwwG_G

Cuts at:      0     185     648 
   Size:        185     463
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     289     540     648 
   Size:        289     251     108

  Fragments arranged by size: 

                 289     251     108
 
TatI w'GTAC_w

Cuts at:      0     471     648 
   Size:        471     177
 
XhoI C'TCGA_G

Cuts at:      0     579     648 
   Size:        579      69
 

 Enzymes that do cut:

     AccI     AcuI    ApaLI     ApoI     AvaI    BfrBI Bme1580I     BsaI
    BseYI  BsiHKAI Bsp1286I   BstAPI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     DraI     EarI  Eco57MI    Hin4I     MmeI     MslI   MspA1I
     NcoI     NsiI     NspI    PflMI     PsiI    PspXI    PvuII     SfcI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI   Bsu36I     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI