(Linear) (Six-base) MAPSORT of: KIEE1711.seq  Check: 5432  from: 1  to: 648



KIEE1711.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:44  ..

AflIII A'CryG_T

Cuts at:      0     272     648 
   Size:        272     376
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     211     648 
   Size:        211     437
 
AlwNI CAG_nnn'CTG

Cuts at:      0      48     648 
   Size:         48     600
 
BanI G'GyrC_C

Cuts at:      0     121     648 
   Size:        121     527
 
BanII G_rGCy'C

Cuts at:      0     598     648 
   Size:        598      50
 
BbeI G_GCGC'C

Cuts at:      0     125     648 
   Size:        125     523
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     330     364     648 
   Size:        330      34     284

  Fragments arranged by size: 

                 330     284      34
 
BlpI GC'TnA_GC

Cuts at:      0     139     648 
   Size:        139     509
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     648 
   Size:        421     227
 
BsaAI yAC'GTr

Cuts at:      0     108     195     648 
   Size:        108      87     453

  Fragments arranged by size: 

                 453     108      87
 
BsaHI Gr'CG_yC

Cuts at:      0     122     210     648 
   Size:        122      88     438

  Fragments arranged by size: 

                 438     122      88
 
BseYI C'CCAG_C

Cuts at:      0     358     635     648 
   Size:        358     277      13

  Fragments arranged by size: 

                 358     277      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     648 
   Size:        217     431
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      92     259     648 
   Size:         92     167     389

  Fragments arranged by size: 

                 389     167      92
 
Bsp1286I G_dGCh'C

Cuts at:      0     598     648 
   Size:        598      50
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     189     648 
   Size:        189     459
 
BssSI C'ACGA_G

Cuts at:      0     436     648 
   Size:        436     212
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     377     648 
   Size:        377     271
 
BstEII G'GTnAC_C

Cuts at:      0     201     648 
   Size:        201     447
 
BstYI r'GATC_y

Cuts at:      0     152     648 
   Size:        152     496
 
DraIII CAC_nnn'GTG

Cuts at:      0      35     648 
   Size:         35     613
 
EarI CTCTTCn'nnn_

Cuts at:      0     361     648 
   Size:        361     287
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     210     588     648 
   Size:        210     378      60

  Fragments arranged by size: 

                 378     210      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     648 
   Size:        421     227
 
HaeII r_GCGC'y

Cuts at:      0     125     531     648 
   Size:        125     406     117

  Fragments arranged by size: 

                 406     125     117
 
KasI G'GCGC_C

Cuts at:      0     121     648 
   Size:        121     527
 
MslI CAynn'nnrTG

Cuts at:      0     325     648 
   Size:        325     323
 
MspA1I CmG'CkG

Cuts at:      0      45     138     362     546     648 
   Size:         45      93     224     184     102

  Fragments arranged by size: 

                 224     184     102      93      45
 
NarI GG'CG_CC

Cuts at:      0     122     648 
   Size:        122     526
 
PmlI CAC'GTG

Cuts at:      0     195     648 
   Size:        195     453
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     430     462     648 
   Size:        430      32     186

  Fragments arranged by size: 

                 430     186      32
 
PvuII CAG'CTG

Cuts at:      0      45     138     546     648 
   Size:         45      93     408     102

  Fragments arranged by size: 

                 408     102      93      45
 
SapI GCTCTTCn'nnn_

Cuts at:      0     361     648 
   Size:        361     287
 
SfcI C'TryA_G

Cuts at:      0     171     648 
   Size:        171     477
 
SfoI GGC'GCC

Cuts at:      0     123     648 
   Size:        123     525
 
StyI C'CwwG_G

Cuts at:      0     537     648 
   Size:        537     111
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     145     648 
   Size:        145     503
 
TatI w'GTAC_w

Cuts at:      0     317     648 
   Size:        317     331
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     258     648 
   Size:        258     390
 
XmnI GAAnn'nnTTC

Cuts at:      0     580     648 
   Size:        580      68
 

 Enzymes that do cut:

   AflIII     AlfI    AlwNI     BanI    BanII     BbeI     BcgI     BlpI
     BpmI    BsaAI    BsaHI    BseYI     BsgI    BsmBI Bsp1286I    BspMI
    BssSI   BstAPI   BstEII    BstYI   DraIII     EarI     EciI  Eco57MI
    HaeII     KasI     MslI   MspA1I     NarI     PmlI     PpiI    PvuII
     SapI     SfcI     SfoI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI
    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BstBI    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI