Restriction map for
pFN21AE1709
(Linear) (Six-base) MAPSORT of: KIEE1709.seq Check: 3813 from: 1 to: 645
KIEE1709.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
ApoI r'AATT_y
Cuts at: 0 58 97 645
Size: 58 39 548
Fragments arranged by size:
548 58 39
AvaI C'yCGr_G
Cuts at: 0 514 645
Size: 514 131
BanI G'GyrC_C
Cuts at: 0 466 645
Size: 466 179
BanII G_rGCy'C
Cuts at: 0 47 628 645
Size: 47 581 17
Fragments arranged by size:
581 47 17
BbeI G_GCGC'C
Cuts at: 0 470 645
Size: 470 175
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 194 645
Size: 86 108 451
Fragments arranged by size:
451 108 86
Bme1580I G_kGCm'C
Cuts at: 0 554 645
Size: 554 91
BmrI ACTGGGnnnn_n'
Cuts at: 0 622 645
Size: 622 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 645
Size: 187 458
BsaI GGTCTCn'nnnn_
Cuts at: 0 525 645
Size: 525 120
BsaHI Gr'CG_yC
Cuts at: 0 467 645
Size: 467 178
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 645
Size: 28 617
Bsp1286I G_dGCh'C
Cuts at: 0 47 554 628 645
Size: 47 507 74 17
Fragments arranged by size:
507 74 47 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 114 543 645
Size: 114 429 102
Fragments arranged by size:
429 114 102
BsrDI GCAATG_nn'
Cuts at: 0 60 645
Size: 60 585
BsrFI r'CCGG_y
Cuts at: 0 521 645
Size: 521 124
BsrGI T'GTAC_A
Cuts at: 0 428 645
Size: 428 217
BssSI C'ACGA_G
Cuts at: 0 329 645
Size: 329 316
BstXI CCAn_nnnn'nTGG
Cuts at: 0 420 645
Size: 420 225
BstYI r'GATC_y
Cuts at: 0 572 645
Size: 572 73
BtgI C'CryG_G
Cuts at: 0 48 273 392 645
Size: 48 225 119 253
Fragments arranged by size:
253 225 119 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 264 645
Size: 264 381
EarI CTCTTCn'nnn_
Cuts at: 0 505 645
Size: 505 140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 645
Size: 187 458
EcoO109I rG'GnC_Cy
Cuts at: 0 126 645
Size: 126 519
EcoRI G'AATT_C
Cuts at: 0 58 645
Size: 58 587
HaeII r_GCGC'y
Cuts at: 0 470 645
Size: 470 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 645
Size: 4 32 609
Fragments arranged by size:
609 32 4
KasI G'GCGC_C
Cuts at: 0 466 645
Size: 466 179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 645
Size: 272 373
MslI CAynn'nnrTG
Cuts at: 0 424 461 645
Size: 424 37 184
Fragments arranged by size:
424 184 37
MspA1I CmG'CkG
Cuts at: 0 594 645
Size: 594 51
NarI GG'CG_CC
Cuts at: 0 467 645
Size: 467 178
NcoI C'CATG_G
Cuts at: 0 392 645
Size: 392 253
NspI r_CATG'y
Cuts at: 0 498 645
Size: 498 147
PflMI CCAn_nnn'nTGG
Cuts at: 0 291 582 645
Size: 291 291 63
Fragments arranged by size:
291 291 63
PfoI T'CCnGG_A
Cuts at: 0 22 89 645
Size: 22 67 556
Fragments arranged by size:
556 67 22
PpuMI rG'GwC_Cy
Cuts at: 0 126 645
Size: 126 519
PstI C_TGCA'G
Cuts at: 0 599 645
Size: 599 46
PvuII CAG'CTG
Cuts at: 0 594 645
Size: 594 51
SapI GCTCTTCn'nnn_
Cuts at: 0 505 645
Size: 505 140
SfcI C'TryA_G
Cuts at: 0 595 645
Size: 595 50
SfoI GGC'GCC
Cuts at: 0 468 645
Size: 468 177
StyI C'CwwG_G
Cuts at: 0 392 645
Size: 392 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 450 645
Size: 450 195
TatI w'GTAC_w
Cuts at: 0 335 428 645
Size: 335 93 217
Fragments arranged by size:
335 217 93
XbaI T'CTAG_A
Cuts at: 0 147 645
Size: 147 498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 460 645
Size: 460 185
Enzymes that do cut:
ApoI AvaI BanI BanII BbeI BbsI Bme1580I BmrI
BpmI BsaI BsaHI BseRI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssSI BstXI BstYI BtgI BtgZI EarI Eco57MI
EcoO109I EcoRI HaeII Hin4I KasI MmeI MslI MspA1I
NarI NcoI NspI PflMI PfoI PpuMI PstI PvuII
SapI SfcI SfoI StyI TaqII TatI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XhoI XmaI XmnI ZraI