Restriction map for
pFN21AE1708
(Linear) (Six-base) MAPSORT of: KIEE1708.seq Check: 1327 from: 1 to: 642
KIEE1708.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 642
Size: 39 603
AflIII A'CryG_T
Cuts at: 0 62 83 218 642
Size: 62 21 135 424
Fragments arranged by size:
424 135 62 21
AhdI GACnn_n'nnGTC
Cuts at: 0 566 642
Size: 566 76
AlwNI CAG_nnn'CTG
Cuts at: 0 118 166 642
Size: 118 48 476
Fragments arranged by size:
476 118 48
ApaLI G'TGCA_C
Cuts at: 0 222 642
Size: 222 420
ApoI r'AATT_y
Cuts at: 0 542 642
Size: 542 100
BanI G'GyrC_C
Cuts at: 0 49 642
Size: 49 593
BanII G_rGCy'C
Cuts at: 0 74 642
Size: 74 568
BbeI G_GCGC'C
Cuts at: 0 53 642
Size: 53 589
BbsI GAAGACnn'nnnn_
Cuts at: 0 565 642
Size: 565 77
BglI GCCn_nnn'nGGC
Cuts at: 0 127 642
Size: 127 515
BglII A'GATC_T
Cuts at: 0 480 642
Size: 480 162
Bme1580I G_kGCm'C
Cuts at: 0 38 226 642
Size: 38 188 416
Fragments arranged by size:
416 188 38
BmgBI CAC'GTC
Cuts at: 0 63 642
Size: 63 579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 642
Size: 127 515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 610 642
Size: 374 236 32
Fragments arranged by size:
374 236 32
BsaAI yAC'GTr
Cuts at: 0 39 642
Size: 39 603
BsaHI Gr'CG_yC
Cuts at: 0 50 642
Size: 50 592
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 642
Size: 70 30 542
Fragments arranged by size:
542 70 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 642
Size: 569 73
BsiHKAI G_wGCw'C
Cuts at: 0 226 642
Size: 226 416
BsmI GAATG_Cn'
Cuts at: 0 181 642
Size: 181 461
Bsp1286I G_dGCh'C
Cuts at: 0 38 74 226 642
Size: 38 36 152 416
Fragments arranged by size:
416 152 38 36
BsrGI T'GTAC_A
Cuts at: 0 215 642
Size: 215 427
BstAPI GCAn_nnn'nTGC
Cuts at: 0 118 642
Size: 118 524
BstYI r'GATC_y
Cuts at: 0 480 642
Size: 480 162
Bsu36I CC'TnA_GG
Cuts at: 0 206 239 642
Size: 206 33 403
Fragments arranged by size:
403 206 33
BtgI C'CryG_G
Cuts at: 0 30 45 74 642
Size: 30 15 29 568
Fragments arranged by size:
568 30 29 15
BtsI GCAGTG_nn'
Cuts at: 0 581 642
Size: 581 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 127 642
Size: 39 88 515
Fragments arranged by size:
515 88 39
EcoO109I rG'GnC_Cy
Cuts at: 0 202 642
Size: 202 440
EcoRV GAT'ATC
Cuts at: 0 231 642
Size: 231 411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 367 631 642
Size: 367 264 11
Fragments arranged by size:
367 264 11
HaeII r_GCGC'y
Cuts at: 0 53 642
Size: 53 589
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 189 221 372 404 552 584 642
Size: 189 32 151 32 148 32 58
Fragments arranged by size:
189 151 148 58 32 32 32
KasI G'GCGC_C
Cuts at: 0 49 642
Size: 49 593
MspA1I CmG'CkG
Cuts at: 0 47 76 115 642
Size: 47 29 39 527
Fragments arranged by size:
527 47 39 29
NarI GG'CG_CC
Cuts at: 0 50 642
Size: 50 592
NspI r_CATG'y
Cuts at: 0 222 642
Size: 222 420
PciI A'CATG_T
Cuts at: 0 218 642
Size: 218 424
PmlI CAC'GTG
Cuts at: 0 39 642
Size: 39 603
SacII CC_GC'GG
Cuts at: 0 48 77 642
Size: 48 29 565
Fragments arranged by size:
565 48 29
SfoI GGC'GCC
Cuts at: 0 51 642
Size: 51 591
SmlI C'TyrA_G
Cuts at: 0 389 625 642
Size: 389 236 17
Fragments arranged by size:
389 236 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 352 642
Size: 352 290
TatI w'GTAC_w
Cuts at: 0 215 642
Size: 215 427
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApaLI ApoI BanI BanII
BbeI BbsI BglI BglII Bme1580I BmgBI BpmI BpuEI
BsaAI BsaHI BsaXI BsgI BsiHKAI BsmI Bsp1286I BsrGI
BstAPI BstYI Bsu36I BtgI BtsI Eco57MI EcoO109I EcoRV
FalI HaeII Hin4I KasI MspA1I NarI NspI PciI
PmlI SacII SfoI SmlI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BlpI BmrI BmtI BplI Bpu10I BsaI BsaBI BsaWI
BseRI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI EcoNI EcoRI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI