(Linear) (Six-base) MAPSORT of: KIEE1707.seq  Check: 8039  from: 1  to: 621



KIEE1707.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:45  ..

AfeI AGC'GCT

Cuts at:      0     146     621 
   Size:        146     475
 
AflIII A'CryG_T

Cuts at:      0     188     621 
   Size:        188     433
 
AlwNI CAG_nnn'CTG

Cuts at:      0     434     621 
   Size:        434     187
 
BfrBI ATG'CAT

Cuts at:      0     172     621 
   Size:        172     449
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     621 
   Size:         67     554
 
BsaHI Gr'CG_yC

Cuts at:      0      32     621 
   Size:         32     589
 
BsaWI w'CCGG_w

Cuts at:      0     150     621 
   Size:        150     471
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     621 
   Size:         91     530
 
BsiEI CG_ry'CG

Cuts at:      0      74     621 
   Size:         74     547
 
BsiHKAI G_wGCw'C

Cuts at:      0      65     621 
   Size:         65     556
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     621 
   Size:         65     556
 
BspEI T'CCGG_A

Cuts at:      0     150     621 
   Size:        150     471
 
BspHI T'CATG_A

Cuts at:      0     311     621 
   Size:        311     310
 
BsrFI r'CCGG_y

Cuts at:      0     110     621 
   Size:        110     511
 
BstYI r'GATC_y

Cuts at:      0      20     621 
   Size:         20     601
 
BtsI GCAGTG_nn'

Cuts at:      0     567     599     621 
   Size:        567      32      22

  Fragments arranged by size: 

                 567      32      22
 
EaeI y'GGCC_r

Cuts at:      0      71     621 
   Size:         71     550
 
EagI C'GGCC_G

Cuts at:      0      71     621 
   Size:         71     550
 
EarI CTCTTCn'nnn_

Cuts at:      0     575     621 
   Size:        575      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     621 
   Size:         67     554
 
HaeII r_GCGC'y

Cuts at:      0     106     148     621 
   Size:        106      42     473

  Fragments arranged by size: 

                 473     106      42
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     157     189     306     338     621 
   Size:        157      32     117      32     283

  Fragments arranged by size: 

                 283     157     117      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     293     621 
   Size:        293     328
 
NotI GC'GGCC_GC

Cuts at:      0      71     621 
   Size:         71     550
 
NsiI A_TGCA'T

Cuts at:      0     174     621 
   Size:        174     447
 
NspI r_CATG'y

Cuts at:      0     192     621 
   Size:        192     429
 
PciI A'CATG_T

Cuts at:      0     188     621 
   Size:        188     433
 
PstI C_TGCA'G

Cuts at:      0     408     621 
   Size:        408     213
 
SfcI C'TryA_G

Cuts at:      0     404     621 
   Size:        404     217
 
SgrAI Cr'CCGG_yG

Cuts at:      0     110     621 
   Size:        110     511
 
StyI C'CwwG_G

Cuts at:      0     278     621 
   Size:        278     343
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      42     621 
   Size:         42     579
 

 Enzymes that do cut:

     AfeI   AflIII    AlwNI    BfrBI     BpmI    BsaHI    BsaWI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BsrFI    BstYI     BtsI
     EaeI     EagI     EarI  Eco57MI    HaeII    Hin4I     MslI     NotI
     NsiI     NspI     PciI     PstI     SfcI    SgrAI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaXI    BseRI    BseYI    BsiWI     BsmI
    BsmBI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NruI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI