Restriction map for
pFN21AE1702
(Linear) (Six-base) MAPSORT of: KIEE1702.seq Check: 6224 from: 1 to: 612
KIEE1702.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AatII G_ACGT'C
Cuts at: 0 419 612
Size: 419 193
AfeI AGC'GCT
Cuts at: 0 179 612
Size: 179 433
AlwNI CAG_nnn'CTG
Cuts at: 0 51 294 363 612
Size: 51 243 69 249
Fragments arranged by size:
249 243 69 51
ApaI G_GGCC'C
Cuts at: 0 103 612
Size: 103 509
ApaLI G'TGCA_C
Cuts at: 0 65 612
Size: 65 547
AvaI C'yCGr_G
Cuts at: 0 220 379 612
Size: 220 159 233
Fragments arranged by size:
233 220 159
BanI G'GyrC_C
Cuts at: 0 493 612
Size: 493 119
BanII G_rGCy'C
Cuts at: 0 25 103 267 353 612
Size: 25 78 164 86 259
Fragments arranged by size:
259 164 86 78 25
BglI GCCn_nnn'nGGC
Cuts at: 0 502 612
Size: 502 110
Bme1580I G_kGCm'C
Cuts at: 0 69 103 612
Size: 69 34 509
Fragments arranged by size:
509 69 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 469 612
Size: 469 143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 592 612
Size: 166 426 20
Fragments arranged by size:
426 166 20
Bpu10I CC'TnA_GC
Cuts at: 0 271 612
Size: 271 341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 333 612
Size: 118 215 279
Fragments arranged by size:
279 215 118
BsaHI Gr'CG_yC
Cuts at: 0 416 612
Size: 416 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 178 208 565 595 612
Size: 178 30 357 30 17
Fragments arranged by size:
357 178 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 612
Size: 238 374
BseYI C'CCAG_C
Cuts at: 0 283 605 612
Size: 283 322 7
Fragments arranged by size:
322 283 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 612
Size: 100 512
BsiHKAI G_wGCw'C
Cuts at: 0 69 612
Size: 69 543
Bsp1286I G_dGCh'C
Cuts at: 0 25 69 103 267 353 612
Size: 25 44 34 164 86 259
Fragments arranged by size:
259 164 86 44 34 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 18 612
Size: 18 594
BsrFI r'CCGG_y
Cuts at: 0 501 612
Size: 501 111
BstAPI GCAn_nnn'nTGC
Cuts at: 0 51 612
Size: 51 561
BstXI CCAn_nnnn'nTGG
Cuts at: 0 96 169 612
Size: 96 73 443
Fragments arranged by size:
443 96 73
BstYI r'GATC_y
Cuts at: 0 158 371 612
Size: 158 213 241
Fragments arranged by size:
241 213 158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 182 612
Size: 182 430
BtsI GCAGTG_nn'
Cuts at: 0 403 612
Size: 403 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 592 612
Size: 166 426 20
Fragments arranged by size:
426 166 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 343 612
Size: 343 269
EcoO109I rG'GnC_Cy
Cuts at: 0 100 291 360 388 540 612
Size: 100 191 69 28 152 72
Fragments arranged by size:
191 152 100 72 69 28
HaeII r_GCGC'y
Cuts at: 0 181 528 612
Size: 181 347 84
Fragments arranged by size:
347 181 84
MspA1I CmG'CkG
Cuts at: 0 48 278 300 411 612
Size: 48 230 22 111 201
Fragments arranged by size:
230 201 111 48 22
NaeI GCC'GGC
Cuts at: 0 503 612
Size: 503 109
NgoMIV G'CCGG_C
Cuts at: 0 501 612
Size: 501 111
PasI CC'CwG_GG
Cuts at: 0 96 260 489 612
Size: 96 164 229 123
Fragments arranged by size:
229 164 123 96
PflMI CCAn_nnn'nTGG
Cuts at: 0 440 612
Size: 440 172
PfoI T'CCnGG_A
Cuts at: 0 182 569 612
Size: 182 387 43
Fragments arranged by size:
387 182 43
PpuMI rG'GwC_Cy
Cuts at: 0 388 540 612
Size: 388 152 72
Fragments arranged by size:
388 152 72
PspOMI G'GGCC_C
Cuts at: 0 99 612
Size: 99 513
PstI C_TGCA'G
Cuts at: 0 47 299 359 410 482 563 612
Size: 47 252 60 51 72 81 49
Fragments arranged by size:
252 81 72 60 51 49 47
PvuII CAG'CTG
Cuts at: 0 48 278 300 411 612
Size: 48 230 22 111 201
Fragments arranged by size:
230 201 111 48 22
SanDI GG'GwC_CC
Cuts at: 0 388 612
Size: 388 224
SbfI CC_TGCA'GG
Cuts at: 0 359 612
Size: 359 253
SfcI C'TryA_G
Cuts at: 0 43 295 355 406 478 559 612
Size: 43 252 60 51 72 81 53
Fragments arranged by size:
252 81 72 60 53 51 43
SmlI C'TyrA_G
Cuts at: 0 133 312 612
Size: 133 179 300
Fragments arranged by size:
300 179 133
StyI C'CwwG_G
Cuts at: 0 395 612
Size: 395 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 31 502 612
Size: 31 471 110
Fragments arranged by size:
471 110 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 178 210 612
Size: 178 32 402
Fragments arranged by size:
402 178 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 612
Size: 33 579
ZraI GAC'GTC
Cuts at: 0 417 612
Size: 417 195
Enzymes that do cut:
AatII AfeI AlwNI ApaI ApaLI AvaI BanI BanII
BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BstAPI
BstXI BstYI BtgZI BtsI Eco57MI EcoNI EcoO109I HaeII
MspA1I NaeI NgoMIV PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SanDI SbfI SfcI SmlI StyI TaqII
TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI
XmnI