Restriction map for
pFN21AE1699
(Linear) (Six-base) MAPSORT of: KIEE1699.seq Check: 886 from: 1 to: 696
KIEE1699.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 388 696
Size: 133 255 308
Fragments arranged by size:
308 255 133
AflII C'TTAA_G
Cuts at: 0 630 696
Size: 630 66
AleI CACnn'nnGTG
Cuts at: 0 16 449 696
Size: 16 433 247
Fragments arranged by size:
433 247 16
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 298 330 696
Size: 298 32 366
Fragments arranged by size:
366 298 32
ApaI G_GGCC'C
Cuts at: 0 494 696
Size: 494 202
ApoI r'AATT_y
Cuts at: 0 362 696
Size: 362 334
BamHI G'GATC_C
Cuts at: 0 338 696
Size: 338 358
BanII G_rGCy'C
Cuts at: 0 494 696
Size: 494 202
BclI T'GATC_A
Cuts at: 0 416 696
Size: 416 280
Bme1580I G_kGCm'C
Cuts at: 0 289 494 696
Size: 289 205 202
Fragments arranged by size:
289 205 202
BmrI ACTGGGnnnn_n'
Cuts at: 0 192 696
Size: 192 504
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 348 696
Size: 348 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 696
Size: 243 453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 696
Size: 318 378
BsaI GGTCTCn'nnnn_
Cuts at: 0 75 696
Size: 75 621
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 575 605 696
Size: 575 30 91
Fragments arranged by size:
575 91 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 156 244 418 571 696
Size: 156 88 174 153 125
Fragments arranged by size:
174 156 153 125 88
BseYI C'CCAG_C
Cuts at: 0 4 487 696
Size: 4 483 209
Fragments arranged by size:
483 209 4
BsiHKAI G_wGCw'C
Cuts at: 0 166 696
Size: 166 530
Bsp1286I G_dGCh'C
Cuts at: 0 166 289 494 696
Size: 166 123 205 202
Fragments arranged by size:
205 202 166 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 641 696
Size: 641 55
BstYI r'GATC_y
Cuts at: 0 338 696
Size: 338 358
Bsu36I CC'TnA_GG
Cuts at: 0 579 696
Size: 579 117
BtgI C'CryG_G
Cuts at: 0 61 696
Size: 61 635
BtsI GCAGTG_nn'
Cuts at: 0 145 696
Size: 145 551
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 243 388 696
Size: 133 110 145 308
Fragments arranged by size:
308 145 133 110
EcoNI CCTnn'n_nnAGG
Cuts at: 0 150 696
Size: 150 546
EcoO109I rG'GnC_Cy
Cuts at: 0 93 306 696
Size: 93 213 390
Fragments arranged by size:
390 213 93
EcoRI G'AATT_C
Cuts at: 0 362 696
Size: 362 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 575 607 696
Size: 220 32 323 32 89
Fragments arranged by size:
323 220 89 32 32
MslI CAynn'nnrTG
Cuts at: 0 16 449 696
Size: 16 433 247
Fragments arranged by size:
433 247 16
NcoI C'CATG_G
Cuts at: 0 61 696
Size: 61 635
NspI r_CATG'y
Cuts at: 0 470 608 696
Size: 470 138 88
Fragments arranged by size:
470 138 88
PasI CC'CwG_GG
Cuts at: 0 572 696
Size: 572 124
PflMI CCAn_nnn'nTGG
Cuts at: 0 201 696
Size: 201 495
PpuMI rG'GwC_Cy
Cuts at: 0 306 696
Size: 306 390
PspOMI G'GGCC_C
Cuts at: 0 490 696
Size: 490 206
PstI C_TGCA'G
Cuts at: 0 476 696
Size: 476 220
SexAI A'CCwGG_T
Cuts at: 0 350 696
Size: 350 346
SfcI C'TryA_G
Cuts at: 0 378 472 621 696
Size: 378 94 149 75
Fragments arranged by size:
378 149 94 75
SmlI C'TyrA_G
Cuts at: 0 333 630 696
Size: 333 297 66
Fragments arranged by size:
333 297 66
SphI G_CATG'C
Cuts at: 0 608 696
Size: 608 88
StyI C'CwwG_G
Cuts at: 0 61 696
Size: 61 635
TatI w'GTAC_w
Cuts at: 0 534 696
Size: 534 162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 68 549 696
Size: 68 481 147
Fragments arranged by size:
481 147 68
Enzymes that do cut:
AcuI AflII AleI AloI ApaI ApoI BamHI BanII
BclI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BstAPI BstYI Bsu36I BtgI
BtsI Eco57MI EcoNI EcoO109I EcoRI Hin4I MslI NcoI
NspI PasI PflMI PpuMI PspOMI PstI SexAI SfcI
SmlI SphI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI XmnI ZraI