Restriction map for
pFN21AE1697
(Linear) (Six-base) MAPSORT of: KIEE1697.seq Check: 3368 from: 1 to: 789
KIEE1697.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 789
Size: 248 541
AflIII A'CryG_T
Cuts at: 0 332 789
Size: 332 457
AleI CACnn'nnGTG
Cuts at: 0 72 789
Size: 72 717
AlwNI CAG_nnn'CTG
Cuts at: 0 249 789
Size: 249 540
ApaLI G'TGCA_C
Cuts at: 0 595 789
Size: 595 194
AvaI C'yCGr_G
Cuts at: 0 653 789
Size: 653 136
BanI G'GyrC_C
Cuts at: 0 554 657 789
Size: 554 103 132
Fragments arranged by size:
554 132 103
BanII G_rGCy'C
Cuts at: 0 232 364 694 789
Size: 232 132 330 95
Fragments arranged by size:
330 232 132 95
BbeI G_GCGC'C
Cuts at: 0 661 789
Size: 661 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 138 372 789
Size: 138 234 417
Fragments arranged by size:
417 234 138
BclI T'GATC_A
Cuts at: 0 752 789
Size: 752 37
BglI GCCn_nnn'nGGC
Cuts at: 0 355 587 789
Size: 355 232 202
Fragments arranged by size:
355 232 202
BlpI GC'TnA_GC
Cuts at: 0 226 789
Size: 226 563
Bme1580I G_kGCm'C
Cuts at: 0 599 789
Size: 599 190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 643 789
Size: 457 186 146
Fragments arranged by size:
457 186 146
Bpu10I CC'TnA_GC
Cuts at: 0 693 789
Size: 693 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 789
Size: 196 593
BsaI GGTCTCn'nnnn_
Cuts at: 0 97 789
Size: 97 692
BsaAI yAC'GTr
Cuts at: 0 315 528 789
Size: 315 213 261
Fragments arranged by size:
315 261 213
BsaHI Gr'CG_yC
Cuts at: 0 658 789
Size: 658 131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 634 664 789
Size: 634 30 125
Fragments arranged by size:
634 125 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 454 640 643 789
Size: 178 276 186 3 146
Fragments arranged by size:
276 186 178 146 3
BseYI C'CCAG_C
Cuts at: 0 185 350 607 661 789
Size: 185 165 257 54 128
Fragments arranged by size:
257 185 165 128 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 789
Size: 25 764
BsiHKAI G_wGCw'C
Cuts at: 0 599 789
Size: 599 190
Bsp1286I G_dGCh'C
Cuts at: 0 232 364 599 694 789
Size: 232 132 235 95 95
Fragments arranged by size:
235 232 132 95 95
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 260 770 789
Size: 260 510 19
Fragments arranged by size:
510 260 19
BsrFI r'CCGG_y
Cuts at: 0 174 548 789
Size: 174 374 241
Fragments arranged by size:
374 241 174
BstAPI GCAn_nnn'nTGC
Cuts at: 0 222 789
Size: 222 567
BstXI CCAn_nnnn'nTGG
Cuts at: 0 435 789
Size: 435 354
BtgI C'CryG_G
Cuts at: 0 16 558 737 789
Size: 16 542 179 52
Fragments arranged by size:
542 179 52 16
BtsI GCAGTG_nn'
Cuts at: 0 276 496 789
Size: 276 220 293
Fragments arranged by size:
293 276 220
DraIII CAC_nnn'GTG
Cuts at: 0 222 789
Size: 222 567
EaeI y'GGCC_r
Cuts at: 0 510 530 789
Size: 510 20 259
Fragments arranged by size:
510 259 20
EarI CTCTTCn'nnn_
Cuts at: 0 331 789
Size: 331 458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 457 643 789
Size: 248 209 186 146
Fragments arranged by size:
248 209 186 146
EcoO109I rG'GnC_Cy
Cuts at: 0 189 453 789
Size: 189 264 336
Fragments arranged by size:
336 264 189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 162 789
Size: 162 627
FspI TGC'GCA
Cuts at: 0 370 789
Size: 370 419
FspAI rTGC'GCAy
Cuts at: 0 370 789
Size: 370 419
HaeII r_GCGC'y
Cuts at: 0 661 789
Size: 661 128
KasI G'GCGC_C
Cuts at: 0 657 789
Size: 657 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 789
Size: 217 572
MscI TGG'CCA
Cuts at: 0 532 789
Size: 532 257
MslI CAynn'nnrTG
Cuts at: 0 72 526 789
Size: 72 454 263
Fragments arranged by size:
454 263 72
MspA1I CmG'CkG
Cuts at: 0 185 354 390 560 789
Size: 185 169 36 170 229
Fragments arranged by size:
229 185 170 169 36
NaeI GCC'GGC
Cuts at: 0 176 789
Size: 176 613
NarI GG'CG_CC
Cuts at: 0 658 789
Size: 658 131
NgoMIV G'CCGG_C
Cuts at: 0 174 789
Size: 174 615
NspI r_CATG'y
Cuts at: 0 262 336 375 435 789
Size: 262 74 39 60 354
Fragments arranged by size:
354 262 74 60 39
PciI A'CATG_T
Cuts at: 0 332 789
Size: 332 457
PfoI T'CCnGG_A
Cuts at: 0 338 789
Size: 338 451
PmlI CAC'GTG
Cuts at: 0 528 789
Size: 528 261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 694 726 789
Size: 694 32 63
Fragments arranged by size:
694 63 32
PpuMI rG'GwC_Cy
Cuts at: 0 189 789
Size: 189 600
PstI C_TGCA'G
Cuts at: 0 767 789
Size: 767 22
PvuII CAG'CTG
Cuts at: 0 185 390 789
Size: 185 205 399
Fragments arranged by size:
399 205 185
SacII CC_GC'GG
Cuts at: 0 561 789
Size: 561 228
SfcI C'TryA_G
Cuts at: 0 763 789
Size: 763 26
SfoI GGC'GCC
Cuts at: 0 659 789
Size: 659 130
SmaI CCC'GGG
Cuts at: 0 655 789
Size: 655 134
SmlI C'TyrA_G
Cuts at: 0 211 789
Size: 211 578
SphI G_CATG'C
Cuts at: 0 262 375 435 789
Size: 262 113 60 354
Fragments arranged by size:
354 262 113 60
StuI AGG'CCT
Cuts at: 0 577 789
Size: 577 212
XmaI C'CCGG_G
Cuts at: 0 653 789
Size: 653 136
XmnI GAAnn'nnTTC
Cuts at: 0 145 789
Size: 145 644
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaLI AvaI BanI BanII
BbeI BbsI BclI BglI BlpI Bme1580I BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstXI BtgI BtsI
DraIII EaeI EarI Eco57MI EcoO109I FalI FspI FspAI
HaeII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmlI PpiI PpuMI PstI
PvuII SacII SfcI SfoI SmaI SmlI SphI StuI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglII
BmgBI BmrI BmtI BplI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI Hin4I HincII HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmeI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI ZraI