Restriction map for
pFN21AE1695
(Linear) (Six-base) MAPSORT of: KIEE1695.seq Check: 6266 from: 1 to: 606
KIEE1695.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 282 606
Size: 282 324
ApaLI G'TGCA_C
Cuts at: 0 367 606
Size: 367 239
AvaI C'yCGr_G
Cuts at: 0 347 356 606
Size: 347 9 250
Fragments arranged by size:
347 250 9
BanI G'GyrC_C
Cuts at: 0 352 606
Size: 352 254
BanII G_rGCy'C
Cuts at: 0 172 606
Size: 172 434
BbeI G_GCGC'C
Cuts at: 0 356 606
Size: 356 250
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 32 66 606
Size: 32 34 540
Fragments arranged by size:
540 34 32
BglI GCCn_nnn'nGGC
Cuts at: 0 72 81 361 606
Size: 72 9 280 245
Fragments arranged by size:
280 245 72 9
BglII A'GATC_T
Cuts at: 0 533 606
Size: 533 73
BlpI GC'TnA_GC
Cuts at: 0 131 606
Size: 131 475
Bme1580I G_kGCm'C
Cuts at: 0 244 371 606
Size: 244 127 235
Fragments arranged by size:
244 235 127
BmgBI CAC'GTC
Cuts at: 0 585 606
Size: 585 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 606
Size: 439 167
BsaI GGTCTCn'nnnn_
Cuts at: 0 379 606
Size: 379 227
BsaAI yAC'GTr
Cuts at: 0 372 606
Size: 372 234
BsaHI Gr'CG_yC
Cuts at: 0 353 606
Size: 353 253
BseYI C'CCAG_C
Cuts at: 0 54 606
Size: 54 552
BsiEI CG_ry'CG
Cuts at: 0 330 606
Size: 330 276
BsiHKAI G_wGCw'C
Cuts at: 0 371 606
Size: 371 235
Bsp1286I G_dGCh'C
Cuts at: 0 172 244 371 606
Size: 172 72 127 235
Fragments arranged by size:
235 172 127 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 282 606
Size: 282 324
BsrBI CCG'CTC
Cuts at: 0 423 458 606
Size: 423 35 148
Fragments arranged by size:
423 148 35
BsrFI r'CCGG_y
Cuts at: 0 80 606
Size: 80 526
BstXI CCAn_nnnn'nTGG
Cuts at: 0 307 606
Size: 307 299
BstYI r'GATC_y
Cuts at: 0 281 533 606
Size: 281 252 73
Fragments arranged by size:
281 252 73
BtgI C'CryG_G
Cuts at: 0 248 606
Size: 248 358
BtsI GCAGTG_nn'
Cuts at: 0 220 606
Size: 220 386
DrdI GACnn_nn'nnGTC
Cuts at: 0 319 606
Size: 319 287
EaeI y'GGCC_r
Cuts at: 0 327 606
Size: 327 279
EagI C'GGCC_G
Cuts at: 0 327 606
Size: 327 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 606
Size: 439 167
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 606
Size: 523 83
EcoO109I rG'GnC_Cy
Cuts at: 0 146 321 606
Size: 146 175 285
Fragments arranged by size:
285 175 146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 573 606
Size: 573 33
HaeII r_GCGC'y
Cuts at: 0 356 606
Size: 356 250
KasI G'GCGC_C
Cuts at: 0 352 606
Size: 352 254
MslI CAynn'nnrTG
Cuts at: 0 247 606
Size: 247 359
MspA1I CmG'CkG
Cuts at: 0 46 435 501 606
Size: 46 389 66 105
Fragments arranged by size:
389 105 66 46
NaeI GCC'GGC
Cuts at: 0 82 606
Size: 82 524
NarI GG'CG_CC
Cuts at: 0 353 606
Size: 353 253
NcoI C'CATG_G
Cuts at: 0 248 606
Size: 248 358
NgoMIV G'CCGG_C
Cuts at: 0 80 606
Size: 80 526
NspI r_CATG'y
Cuts at: 0 229 606
Size: 229 377
PasI CC'CwG_GG
Cuts at: 0 10 237 606
Size: 10 227 369
Fragments arranged by size:
369 227 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 588 606
Size: 588 18
PfoI T'CCnGG_A
Cuts at: 0 506 587 606
Size: 506 81 19
Fragments arranged by size:
506 81 19
PmlI CAC'GTG
Cuts at: 0 372 606
Size: 372 234
PpuMI rG'GwC_Cy
Cuts at: 0 146 321 606
Size: 146 175 285
Fragments arranged by size:
285 175 146
PstI C_TGCA'G
Cuts at: 0 191 573 606
Size: 191 382 33
Fragments arranged by size:
382 191 33
PvuII CAG'CTG
Cuts at: 0 46 435 606
Size: 46 389 171
Fragments arranged by size:
389 171 46
SbfI CC_TGCA'GG
Cuts at: 0 191 606
Size: 191 415
SfcI C'TryA_G
Cuts at: 0 187 569 606
Size: 187 382 37
Fragments arranged by size:
382 187 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 81 606
Size: 81 525
SfoI GGC'GCC
Cuts at: 0 354 606
Size: 354 252
StyI C'CwwG_G
Cuts at: 0 113 248 606
Size: 113 135 358
Fragments arranged by size:
358 135 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 403 468 606
Size: 403 65 138
Fragments arranged by size:
403 138 65
Tth111I GACn'n_nGTC
Cuts at: 0 478 606
Size: 478 128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 225 606
Size: 225 381
Enzymes that do cut:
AarI ApaLI AvaI BanI BanII BbeI BcgI BglI
BglII BlpI Bme1580I BmgBI BpmI BsaI BsaAI BsaHI
BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstXI
BstYI BtgI BtsI DrdI EaeI EagI Eco57MI EcoNI
EcoO109I FalI HaeII KasI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI
PstI PvuII SbfI SfcI SfiI SfoI StyI TaqII
Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BciVI BclI BfrBI BmrI BmtI BplI Bpu10I BpuEI
BsaBI BsaWI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EarI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI ScaI SexAI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI XbaI XhoI XmaI
XmnI ZraI